A5076529093- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Micro and Nano Robotics
- Immune cells in cancer
- Cardiomyopathy and Myosin Studies
- Nanoplatforms for cancer theranostics
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
Charles River Laboratories (United States)
2024-2025
University of San Diego
2021-2023
Differential dynamic microscopy, image autocorrelation, and mechanistic modeling show that actomyosin–microtubule composites exhibit tunable ballistic contraction restructuring with microtubules enabling emergent sustained controlled dynamics.
Abstract The application of new in vivo animal models with greater translational relevance is important for successful drug development. Severely immunodeficient mouse strains engineered enhanced engraftment and persistence diverse human immune cell types are now widely used discovery across different therapeutic areas. These allow testing modulating agents that target specific components the system, including both lymphoid myeloid lineages. They have been particularly useful development...
The cellular cytoskeleton relies on diverse populations of motors, filaments, and binding proteins acting in concert to enable nonequilibrium processes ranging from mitosis chemotaxis. cytoskeleton's versatile reconfigurability, programmed by interactions between its constituents, makes it a foundational active matter platform. However, current endeavors are limited largely single force-generating components substrate-far the composite cells. Here, we engineer actin-microtubule (MT)...
The composite cytoskeleton, comprising interacting networks of semiflexible actin and rigid microtubules, actively generates forces restructures using motor proteins such as myosins to enable key mechanical processes including cell motility mitosis. Yet, how motor-driven activity alters the mechanics cytoskeleton composites remains an open challenge. Here, we perform optical tweezers microrheology on actin-microtubule driven by myosin II motors show that increases linear viscoelasticity...
Abstract The cytoskeleton of biological cells relies on a diverse population motors, filaments, and binding proteins acting in concert to enable non-equilibrium processes ranging from mitosis chemotaxis. cytoskeleton’s versatile reconfigurability, programmed by interactions between its constituents, make it foundational active matter platform. However, current endeavors are limited largely single force-generating components substrate – far the composite live cells. Here, we engineer...
Abstract Translational in vivo models are needed to expedite the discovery of new treatments for lung cancer. One such approach is use humanized mouse that enable immunotherapies targeting human immune cells be evaluated efficacy and tolerability. We developed an orthotopic carcinoma model mice (HuCD34 NCG), utilizing serial bioluminescence imaging evaluate activity paclitaxel pembrolizumab as single combination agents. Paclitaxel, which disrupts microtuble function, pembrolizumab, a...
The cytoskeleton is a model active matter system that controls diverse cellular processes from division to motility. While both actomyosin dynamics and actin-microtubule interactions are key the cytoskeleton’s versatility adaptability, an understanding of their interplay lacking. Here, we couple microscale experiments with mechanistic modeling elucidate how connectivity, rigidity, force-generation affect emergent material properties in vitro composites actin, tubulin, myosin. We use...
Abstract The cytoskeleton is a model active matter system that controls diverse cellular processes from division to motility. While both actomyosin dynamics and actin-microtubule interactions are key the cytoskeleton’s versatility adaptability, an understanding of their interplay lacking. Here, we couple microscale experiments with mechanistic modeling elucidate how connectivity, rigidity, force-generation affect emergent material properties in vitro composites actin, tubulin, myosin. We use...