A5052854787- Cardiomyopathy and Myosin Studies
- Pluripotent Stem Cells Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Salmonella and Campylobacter epidemiology
- CRISPR and Genetic Engineering
- Chemokine receptors and signaling
- Viral Infectious Diseases and Gene Expression in Insects
- Muscle Physiology and Disorders
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- Congenital heart defects research
- Neuroscience and Neural Engineering
- Viral Infections and Immunology Research
- Vibrio bacteria research studies
- Virus-based gene therapy research
- Helicobacter pylori-related gastroenterology studies
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Signaling Pathways in Disease
- Cellular Mechanics and Interactions
- Bacteriophages and microbial interactions
- Cytomegalovirus and herpesvirus research
Medizinische Hochschule Hannover
2004-2024
Max Planck Society
2001-2002
Max Planck Institute for Infection Biology
2001
Max Planck Institute of Biochemistry
1999
Humoral immunity in the gut-associated lymphoid tissue is characterized by production of immunoglobulin A (IgA) antibody-secreting plasma cells (PCs) lamina propria. The chemokine CCL25 expressed intestinal epithelial and capable inducing chemotaxis IgA+ PCs vitro. Using a newly generated monoclonal antibody against murine CCR9, we show that express high levels CCR9 mesenteric lymph node (MLN) Peyer's patches (PPs), but down-regulate once they are located small intestine. In CCR9-deficient...
Small intestine plasmacytoid dendritic cells (pDC) are poorly characterized. Here, we demonstrate that intestinal pDC show the characteristic plasma cell-like morphology, and recognized by antibodies against B220, Ly6c, 120G8, PDCA-1, markers typically expressed pDC. Furthermore, carry high levels of CCR9 largely absent in intestine, but not lung, liver, or secondary lymphoid organs CCR9-deficient animals. Competitive adoptive transfers reveal impaired homing to small after i.v. transfer. In...
ABSTRACT Secreted proteins (the secretome) of the human pathogen Helicobacter pylori may mediate important pathogen-host interactions, but such are technically difficult to analyze. Here, we report on a comprehensive secretome analysis that uses protein-free culture conditions minimize autolysis, an efficient recovery method for extracellular proteins, and two-dimensional gel electrophoresis followed by peptide mass fingerprinting protein resolution identification. Twenty-six 33 separated...
Characterizing the contractile function of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) is key for advancing their utility cellular disease models, promoting cell based heart repair, or developing novel pharmacological interventions targeting cardiac diseases. The aim present study was to understand whether steady-state and kinetic force parameters β-myosin heavy chain (βMyHC) isoform-expressing myofibrils within embryonic (hESC-CMs) differentiated in vitro resemble those...
ABSTRACT The adeno-associated virus type 2 (AAV-2) Rep78/Rep68 regulatory proteins are pleiotropic effectors of viral and cellular DNA replication, transformation by oncogenes, homologous heterologous gene expression. To search for involved in mediating these functions, we used Rep68 as bait the yeast two-hybrid system identified transcriptional coactivator PC4 a Rep interaction partner. has been shown to mediate activation variety sequence-specific transcription factors vitro. amino acids...
Green fluorescent protein (GFP) is an attractive reporter for Salmonella gene expression analysis but might interfere with virulence when expressed at high levels. To identify suitable GFP levels, we constructed a series of strains expressing different amounts and measured their fluorescence colonization levels in infected mice. The results show that concentrations the range 7000-200,000 molecules per cell are detectable ex vivo samples using flow cytometry, cause no major defect....
Deficiency of transplant recipients for the chemokine receptor CCR7 was originally described to slightly increase survival time vascularized solid organ grafts, probably due a reduced priming alloreactive T cells. Using model allotolerance induction by donor-specific splenocyte transfusion (DST) in combination with anti-CD40L mAb-mediated costimulation blockade (CSB), we show here striking failure CCR7-deficient (CCR7(-/-) ) tolerate cardiac allografts. Furthermore, addition recently lack...
Myosin heavy chain (MyHC) is the main determinant of contractile function. Human ventricular cardiomyocytes (CMs) predominantly express β-isoform. We previously demonstrated that ∼80% human embryonic stem cell-derived (hESC-CMs) exclusively β-MyHC after long-term culture on laminin-coated glass coverslips. Here, we investigated impact enzymatically detaching hESC-CMs and subsequently replating them for characterization cellular observed force-related kinetic parameters, as measured in a...
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) represent an attractive model to investigate CM function and disease mechanisms. One characteristic marker of ventricular specificity human CMs is expression the ventricular, slow β-myosin heavy chain (MyHC), as opposed atrial, fast α-MyHC. The main aim this study was at single-cell level whether contraction kinetics electrical activity hESC-CMs are influenced by relative α-MyHC versus β-MyHC. For effective assignment functional...