A5077275635- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Antibiotics Pharmacokinetics and Efficacy
- Analytical chemistry methods development
- Pharmacological Effects and Assays
- Advanced Nanomaterials in Catalysis
- Food composition and properties
- Mycotoxins in Agriculture and Food
- Identification and Quantification in Food
- Pharmaceutical and Antibiotic Environmental Impacts
- Polysaccharides Composition and Applications
- Meat and Animal Product Quality
- Advanced Biosensing Techniques and Applications
- MicroRNA in disease regulation
- Advanced Chemical Sensor Technologies
- Antibiotic Resistance in Bacteria
- Rabbits: Nutrition, Reproduction, Health
- Drug Transport and Resistance Mechanisms
- RNA modifications and cancer
- Listeria monocytogenes in Food Safety
- Ferroptosis and cancer prognosis
- SARS-CoV-2 detection and testing
- Proteins in Food Systems
- Synthesis and Biological Evaluation
- Mass Spectrometry Techniques and Applications
Southwest University
2016-2025
Ministry of Education
2025
Département Santé Animale
2025
South China Agricultural University
2020-2022
University of Waterloo
2020
Ministry of Education of the People's Republic of China
2019
Xinjiang Academy of Forestry
2015
China Agricultural University
2009-2014
Developing a specific, sensitive, rapid, and on-site method for detecting pathogenic bacteria in food samples is critical to ensuring public safety. This article demonstrates CRISPR/Cas13a system chemiluminescence resonance energy transfer (CRET) (CRISPR/Cas 13a-assisted CRET)-based strategy sensitive detection of real samples. Once the hybrid double strand aptamerS. aureus-cRNA recognizes target model Staphylococcus aureus (S. aureus), released cRNA would bind with CRISPR/Cas 13a form...
Nanoparticles (NPs) can make their way to the brain and cause in situ damage, which is a concern for nanomaterial application airborne particulate matter exposure. Our recent study indicated that respiratory exposure silica nanoparticles (SiO2 NPs) caused unexpected cardiovascular toxic effects. However, toxicities of SiO2 NPs other organs have warranted further investigation. To confirm accumulation brain, we introduced with different diameters into mice via intranasal instillation (INI)...
The intracellular invasion and survival of a pathogen like Staphylococcus aureus (S. aureus) within host cells enable them to resist antibiotic treatment colonize long-term in the host, which leads series clinical issues. Rapid specific detection bacteria is important diagnosis infection guiding administration. Herein, this work reports simple one-step fluorescence resonance energy transfer (FRET) platform-based strategy achieve rapid S. specimens phagocytic cells. aptamer modified quantum...
Bacterial resistance to fluoroquinolones result from mutations in the quinolone resistance-determining regions of drug targets, overexpression efflux pumps, and/or more recently identified plasmid-mediated low-level mechanisms. We investigated prevalence and characterized fluoroquinolone genes (qnrA, qnrB, qnrS, aac(6')-Ib-cr, qepA) by polymerase chain reaction fluoroquinolone-resistant Escherichia coli (n = 530) isolated a chicken farm, pig hospitalized patients Shandong, China, 2007. The...
A competitive, direct, chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for chloramphenicol (CAP) residues in milk, milk powder, honey, eggs and chicken muscle has been developed. The method gave a detection limit of 0.7 ng L−1 linear range 2.1–92.4 L−1, with the IC50 13.6 under optimal conditions, dramatically better than any previously reported ELISA CAP detection. Spiked at levels 5–60 different food samples, recoveries were 72.1–116.0%, coefficient variations 4.2–20.2%. In...
Label selection is an essential procedure for improving the sensitivity of fluorescence immunochromatography assays (FICAs). Under optimum conditions, time-resolved fluorescent nanobeads (TRFN), quantum dots (QB) and (QD)-based (TRFN-FICA, QB-FICA QD-FICA) were systematically comprehensively compared quantitative detection aflatoxin B1 (AFB1) in six grains (corn, soybeans, sorghum, wheat, rice oat). All three FICAs can be applied as rapid, cost-effective convenient qualitative tools onsite...
Our work suggested that cuproptosis serves as a key toxic mechanism of CuO NPs, damaging mitochondria to induce ferroptosis by disrupting mitochondrial membrane lipid synthesis.
Major research efforts are focusing on the development of simultaneous multiplexed immunoassays. In this study, a novel dual-binding fluorescence polarisation immunoassay (DB-FPIA) using broad-specificity bi-specific single-chain diabody (scDb) and two fluorescent-labelled tracers (sulfamethoxypyridazine-fluorescein isothiocyanate (SMP-FITC) sarafloxacin-Texas Red (SAR-TR)) with different excitation emission wavelengths was developed for high-throughput detection 19 fluoroquinolones (FQs) 13...
A quadruple-label time-resolved fluorescence immunochromatographic assay (QL-TRFICA) for simultaneous quantitative detection of three mycotoxins (AFB<sub>1</sub>, ZEN and OTA) in 6 grains (corn, soybean, sorghum, wheat, rice oats) was established.
The formation of a protein corona (PC) can significantly impact the detection ability gold nanoparticles (AuNPs)-small molecule adsorbent probes based on competitive adsorption. To alleviate this problem, in study, double-stranded DNA (dsDNA) was introduced to modify AuNP mitigate negative effect PCs small molecules by taking AuNPs-dichlorofluorescein (DCF) probe-based ambroxol hydrochloride (AMB) as study model. It found that dsDNA-modified AuNPs-DCF probe (dsDNA@AuNPs-DCF), accuracy for...