A5016217874- Animal Nutrition and Physiology
- Gut microbiota and health
- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Animal Genetics and Reproduction
- Chromosomal and Genetic Variations
- Liver Disease Diagnosis and Treatment
- Agriculture, Soil, Plant Science
- Metabolomics and Mass Spectrometry Studies
- Probiotics and Fermented Foods
- Nutrition, Genetics, and Disease
- Enterobacteriaceae and Cronobacter Research
- MicroRNA in disease regulation
- Clostridium difficile and Clostridium perfringens research
- RNA Research and Splicing
- Peroxisome Proliferator-Activated Receptors
- Protein Hydrolysis and Bioactive Peptides
- Cholesterol and Lipid Metabolism
- Reproductive tract infections research
China Agricultural University
2021-2025
Ministry of Agriculture and Rural Affairs
2021-2025
The gene numbers and evolutionary rates of birds were assumed to be much lower than those mammals, which is in sharp contrast the huge species number morphological diversity birds. It is, therefore, necessary construct a complete avian genome analyze its evolution. We constructed chicken pan-genome from 20 de novo assembled genomes with high sequencing depth, identified 1,335 protein-coding genes 3,011 long noncoding RNAs not found GRCg6a. majority these novel detected across most...
The gut microbiota of chickens plays an important role in host physiology. However, the colonization and prevalence have not been well-characterized. Here, we performed 16S rRNA gene sequencing on duodenal, cecal fecal broilers at 1, 7, 21, 35 days age characterized dynamic succession across intestinal tract. Our results showed that Firmicutes was most abundant phylum detected each site various ages, while microbial diversity composition varied among duodenum, cecum, feces different ages....
Abstract Chicken is a valuable model for understanding fundamental biology, vertebrate evolution and diseases, as well major source of nutrient-dense lean-protein-enriched food globally. Although it the first non-mammalian amniote genome to be sequenced, chicken still lacks systematic characterization functional impacts genetic variants. Here, through integrating 7,015 RNA-Seq 2,869 whole-genome sequence data, Genotype- Tissue Expression (ChickenGTEx) project presents pilot reference...
The microbiota of female reproductive tract have attracted considerable attention in recent years due to their effects on host fitness. However, the throughout chicken oviduct and its symbiotic relationships with not been well characterized. Here, we characterized microbial composition six segments tract, including infundibulum, magnum, isthmus, uterus, vagina cloaca, pedigreed laying hens phenotypes egg quality quantity. We found that diversity gradually increased along from infundibulum...
Blood biochemical indicators play a crucial role in assessing an individual's overall health status and metabolic function. In this study, we measured five blood indicators, including total cholesterol (CHOL), low-density lipoprotein (LDL-CH), triglycerides (TG), high-density (HDL-CH), glucose (BG), as well 19 growth traits of 206 male chickens. By integrating host whole-genome information 16S rRNA sequencing the duodenum, jejunum, ileum, cecum, feces microbiota, assessed contributions...
Abstract Hepatic steatosis is the initial manifestation of abnormal liver functions and often leads to diseases such as nonalcoholic fatty disease in humans syndrome animals. In this study, we conducted a comprehensive analysis large chicken population consisting 705 adult hens by combining host genome resequencing; transcriptome, proteome, metabolome analysis; microbial 16S ribosomal RNA gene sequencing each gut segment. The results showed heritability (h2 = 0.25) duodenal microbiability...
Improving feed efficiency is an important target for poultry breeding. Feed affected by host genetics and the gut microbiota, but many of mechanisms remain elusive in laying hens, especially late period. In this study, we measured intake, body weight, egg mass 714 hens from a pedigreed line 69 to 72 wk age calculated residual intake (RFI) conversion ratio (FCR). addition, fecal samples were also collected 16S ribosomal RNA gene sequencing (V4 region). Genetic analysis was then conducted DMU...
Abstract Background The breeding of layers emphasizes the continual selection egg-related traits, such as egg production, quality and eggshell, which enhance their productivity meet demand market. As process continued, genomic homozygosity gradually increased, resulting in emergence runs (ROH). Therefore, ROH analysis can be used conjunction with other methods to detect signatures identify candidate genes associated various important traits layer breeding. Results In this study, we generated...
The complex interactions between host genetics and the gut microbiome are well documented. However, specific impacts of gene expression patterns microbial composition on each other remain to be further explored. Here, we investigated this interplay in a sizable population 705 hens, employing integrative analyses examine relationships among genome, mucosal expression, microbiota. Specific taxa, such as cecal family Christensenellaceae, which showed heritability 0.365, were strongly correlated...
Eggshell is predominantly composed of calcium carbonate, making up about 95% its composition. quality closely related to the amount deposition in shell, which requires chickens maintain a robust state metabolism. In this study, we introduced novel parameter, Total Weight (TESW), measures total weight eggshells produced by over period 10 consecutive d, providing valuable information on intensity metabolism chickens. Genome-wide association study (GWAS) was conducted explore genetic...
The internal quality of eggs is critical for human consumption and embryonic development. However, microorganisms inside have not been thoroughly investigated their roles in determining the egg's quality. Here, a total 21 hens were selected from more than 1,000 chickens based on hatching results divided into high- low-hatchability groups. Then, we collected 72 these to obtain egg whites yolks, including 54 fresh 18 after 12 days incubation. We characterized microbial composition yolks...
Hepatic steatosis is a prevalent manifestation of fatty liver, that has detrimental effect on the health and productivity laying hens, resulting in economic losses to poultry industry. Here, we aimed systematically investigate genetic regulatory mechanisms hepatic hens.Ninety individuals with most prominent characteristics were selected from 686 hens according accumulation lipid droplets graded into three groups, including control, mild severe groups. A combination transcriptome, proteome,...
Abstract The gene numbers and evolutionary rates of birds were assumed to be much lower than that mammals, which in sharp contrast the huge species number morphological diversity birds. It is very necessary construct a complete avian genome analyze its evolution.We constructed chicken pan-genome from 20 de novo assemblies with high sequencing depth, newly identified 1,335 protein-coding genes 3,011 long noncoding RNAs. majority these novel detected across most individuals examined...
Abstract Background Embryonic diapause (dormancy) is a state of temporary arrest embryonic development that triggered by unfavorable conditions and serves as an evolutionary strategy to ensure reproductive survival. Unlike maternally-controlled in mammals, chicken critically dependent on the environmental temperature. However, molecular control avian species remains largely uncharacterized. In this study, we evaluated dynamic transcriptomic phosphoproteomic profiles embryos pre-diapause,...
<title>Abstract</title> Chicken is a valuable model for understanding fundamental biology and vertebrate evolution, as well major source of nutrient-dense lean-protein-enriched food globally. Although it the first non-mammalian amniote genome to be sequenced, chicken still lacks systematic characterization functional variation. Here, through integrating bulk RNA-Seq 7,015 samples, single-cell 127,598 cells 2,869 whole-genome sequences, we presented an atlas regulatory variants across 28...
Abstract The complex interactions between host genetics and the gut microbiome are well recognized; however, specific impact of gene expression pattern microbial composition on each other remains to be further explored. Here we investigated this interplay within a sizable population 705 hens, employing integrative analyses examine relationships among genetics, mucosal expression, microbiota. Specific taxa exhibited strong adherence genomic variants, particularly in cecum such as...
Proteins in egg whites play vital roles embryonic development. Simultaneously, protein modification is affected by the surrounding environment, which ultimately affects structure and function of proteins. Here, we measured phenotypes eggs at different time points during storage incubation used 4D label-free quantitative proteomics technology liquid chromatography/tandem mass spectrometry (LC-MS/MS)-technique to identify differential proteins N-glycosylation sites incubation. We found that...