A5110549255- Cellular Mechanics and Interactions
- Skin Protection and Aging
- 3D Printing in Biomedical Research
- Biochemical effects in animals
- Wound Healing and Treatments
- Dermatologic Treatments and Research
- Bone Tissue Engineering Materials
- Free Radicals and Antioxidants
- Body Contouring and Surgery
- Additive Manufacturing and 3D Printing Technologies
- Stress Responses and Cortisol
- Reconstructive Surgery and Microvascular Techniques
- Electrospun Nanofibers in Biomedical Applications
- Advancements in Transdermal Drug Delivery
- Silk-based biomaterials and applications
- Planarian Biology and Electrostimulation
- Bee Products Chemical Analysis
Vitrociset (Italy)
2021-2024
Center for Advanced Biomaterials for Healthcare
2018-2022
Italian Institute of Technology
2018-2022
University of Naples Federico II
2018
Utra Violet type A (UVA) exposure strongly affects the ageing of human skin by modifying both epidermis and dermis their cross talk as well. The possibility to get a deep understanding in vitro such crucial mechanism would have huge impact development antiageing compounds. Here, we present full thickness model equivalent formed millimeter-sized completely composed fibroblasts embedded own extracellular matrix. We show that endogenous nature compartment allows replication complexity mutual...
Carnosine is an endogenous β-alanyl-L-histidine dipeptide endowed with antioxidant and carbonyl scavenger properties, which able to significantly prevent the visible signs of aging photoaging. To investigate mechanism action carnosine on human skin proteome, a 3D scaffold-free spheroid model primary dermal fibroblasts from 50-year-old donor was adopted in combination quantitative proteomics for first time. The label free approach based high-resolution mass spectrometry, integrated network...
Dermis fibroblasts are very sensitive to penetrating UVA radiation and induce photo-damage. To protect skin cells against this environmental damage, there is an urgent need for effective compounds, specifically targeting UVA-induced mitochondrial injury. This study aimed analyze the effect of carnosine on proteome UVA-irradiated human fibroblast, cultured in a three-dimensional (3D) biological system recapitulating dermal compartment as test investigate altered cellular pathways after 48 h 7...
Extracellular matrix assembly and composition influence the biological mechanical functions of tissues. Developing strategies to control spatial arrangement cells is central importance for tissue engineering-related approaches relying on self-assembling scaffoldless processes. Literature reports demonstrated that signals patterned material surfaces are able cell positioning orientation. However, mechanisms underlying interactions between structure de novo synthesized far from being...
Dermis spheroids from different donors (40 and 50 years old) were developed primary fibroblasts to demonstrate their capacity synthetize organize the main dermal structural components when cultured in 3D microenvironment, forming endogenous de novo ECM according potential metabolic activity.Dermis produced human at early passages hanging drop culture system. models characterized terms of spheroid diameter, PICP release, collagen III CD44 expression.An increase synthesis (101%) was found...
Backgrounds/Objectives: Skin wound healing is a physiological process orchestrated by epithelial and mesenchymal cells able to restore tissue continuity re-organizing themselves the ECM. This research study aimed develop an optimized in vitro experimental model of full-thickness skin, address molecular morphological modifications occurring re-epithelization process. Methods: Wound starting events were investigated within window 8 days at level gene expression immunofluorescence key epidermal...
The current necessity of development new biological in vitro models that mimic the characteristics and complexity human tissue arises from need to find a valid alternative animal test validate products, screen substances procedures for repair regeneration. In particular, cosmetics field, big multinational companies have developed devised methods realization testing platforms large scale, since European Regulations (Decree 76/768/EEC EU Cosmetic Regulation 1223/2009) ban putting on market...