A5034340622- Wastewater Treatment and Nitrogen Removal
- Plasma Diagnostics and Applications
- Microbial Community Ecology and Physiology
- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Plasma Applications and Diagnostics
- Ion-surface interactions and analysis
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Pulsed Power Technology Applications
- ATP Synthase and ATPases Research
- Hydrogen Storage and Materials
- Electrokinetic Soil Remediation Techniques
- GaN-based semiconductor devices and materials
- Semiconductor materials and devices
- Ammonia Synthesis and Nitrogen Reduction
- Electrostatic Discharge in Electronics
- Cell Image Analysis Techniques
- Sulfur Compounds in Biology
- Gut microbiota and health
- Neural dynamics and brain function
- Digital Holography and Microscopy
- Coagulation and Flocculation Studies
- Neuroendocrine regulation and behavior
Wuhan National Laboratory for Optoelectronics
2020-2025
Huazhong University of Science and Technology
2020-2025
Xi'an University of Technology
2025
Southeast University
2023-2024
Shanghai Industrial Technology Institute
2024
Despite the prevalence of superresolution (SR) microscopy, quantitative live-cell SR imaging that maintains completeness delicate structures and linearity fluorescence signals remains an uncharted territory. Structured illumination microscopy (SIM) is ideal tool for imaging. However, it suffers from out-of-focus background leads to reconstruction artifacts. Previous post hoc suppression methods are prone human bias, fail at densely labeled structures, nonlinear. Here, we propose a physical...
Golgi-derived PI4P-containing vesicles play important roles in mitochondrial division, which is essential for maintaining cellular homeostasis. However, the mechanism of vesicle effect on division unclear. Here, we found that actin appeared to polymerize at contact site between and mitochondria, causing division. Increasing content PI4P derived from Golgi apparatus increased polymerization reduced length suggesting through involved vesicle-related Collectively, our results support a model...
As the most important organ in our bodies, brain plays critical roles deciding sex-related differential features; however, underlying neural circuitry basis remains unclear. Here, we used cell-type-specific rabies virus-mediated monosynaptic tracing systems to generate sex differences-related whole-brain input atlas of locus coeruleus noradrenaline (LC-NE) neurons. We developed custom pipelines for brain-wide comparisons sources both sexes with registration data set Allen Mouse Brain...
Super-resolution fluorescence microscopy has emerged as a powerful tool for studying endoplasmic reticulum (ER) dynamics in living cells. However, the lack of high-brightness, high-photostability, and stable labeling probes makes long-term super-resolution imaging ER still challenging. Herein, we reported surface-functionalized Halo-tag gold nanofluorescent probe (GNP-Atto565-fR8-CA) that exhibits excellent brightness, photostability, biocompatibility. GNP-Atto565-fR8-CA can simultaneously...
Ammonia, as one of the most important chemicals in world, is mainly synthesized through energy-intensive Haber-Bosch process. In recent years, researchers have been committed to exploring efficient catalysts...
Dynamic monitoring of hypochlorous acid (HClO) in mitochondria is crucial for elucidating the molecular pathogenesis ferroptosis-related diseases. Super-resolution microscopy, which surpasses optical diffraction limit, has emerged as a powerful tool subcellular dynamic imaging. However, lack mitochondrial HClO fluorescent probes with high specificity, stable labeling, low environmental interference, and negligible spectral crosstalk presents significant challenge achieving super-resolution...
Because of their excellent photostability and brightness, organic fluorophores are becoming increasingly attractive for long-term live-cell superresolution imaging subcellular structures but often suffer from low cell permeability. Here, we develop a general strategy to deliver cell-impermeable fluorescent probes, including various SNAP/Halo tag into live cells with delivery efficiency (up 97% labeling) almost 100% endo/lysosomal release by simple coincubation novel type peptide vehicle,...
Vesicle entrapment remains a major bottleneck for the application of fluorescent nanoprobes (FNPs) with excellent optical properties in field super-resolution imaging subcellular structures living cells. Here, we developed cell-penetrating peptide, fR8, which enables efficient cytoplasmic delivery 3.5 nm FNPs instead being trapped vesicles, efficiency up to 75.6%. Further, have successfully combined fusion protein technology FNPs-fR8 achieve high specificity and stable labeling various...
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The hollow-cathode nanosecond pulsed electron beam changes the components and organization of material surface through thermal coupling prepares a modified layer on base with greatly optimized physicochemical mechanical properties, which has wide range applications. In this study, in view insufficient research heat source model beam, combined is proposed for first time by abstracting action as two sets Gaussian sources different parameters, equating high-energy body source, irradiation...
Improving the high-voltage resistance of discharge cavity is essential to enhancing performance hollow-cathode electron source. In this work, a beam source designed and optimized by stacking isolating insulating sheets. The experimental results indicate that design not only improves overall device but also stability discharge. Meanwhile, it found when amplitude breakdown voltage increased from 12 kV 20 kV, varies within range 3.8–3.7 Ω, with slight change in amplitude, inductance decreases...