Paul Speliakos
A5114563960- Cancer Immunotherapy and Biomarkers
- Glioma Diagnosis and Treatment
- Brain Metastases and Treatment
- Immunotherapy and Immune Responses
Dana-Farber Cancer Institute
2024
<p>Supplementary Figure 7. Cellular Neighborhood Analyses. Comparison of annotated cellular neighborhoods in pre- and post-treatment tumor samples via CODEX (color code as 4).</p>
<p>Supplementary Figure 3. Digital quantification from immunohistochemistry. immunohistochemistry for T cell subsets and marker analysis paired tumor samples five patients enrolled to cohort A.</p>
<p>Supplementary Figure 6. Cell-cell interaction analyses. comparison in pre- and post-treatment tumor samples using CODEX for patient #5. (A) All significant interacting cell types shown the entire analyzed region as annotated. (B) Example images of – interactions showing CD163+ macrophages with GZMB+ CD8 T cells, CD4 CD68+ cells. The plus sign indicates that were close proximity at a one-cell distance (20 pixels).</p>
<p>Supplementary Figure 2. Patient enrollment, treatment and disposition per cohort.</p>
<p>Supplementary Figure 4. CODEX analysis of tumor PD-L1 expression patterns. (A) was detected in NCAM-1+ cells surrounding by hypoxia region as CA9 staining. (B) also observed GFAP+ surrounded the vicinity apoptotic (cleaved caspase-3 detection).</p>
<div>AbstractPurpose:<p>Radiotherapy may enhance antitumor immune responses by several mechanisms, including induction of immunogenic cell death. We performed a phase 2 study pembrolizumab with re-irradiation in patients recurrent glioblastoma.</p>Patients and Methods:<p>Sixty glioblastoma received alone (cohort A, bevacizumab-naïve; <i>n</i> = 30) or bevacizumab continuation B, 30). Dual primary endpoints, the overall response rate survival (OS) at either...
<p>Supplementary Figure 1. CODEX panel and experimental procedure. used to identify tumor cell subpopulations, immune populations key cellular markers. (B) Experimental workflow for 5 pre- post-treatment FFPE samples. ROIs were selected multiplexed imaging downstream analysis. (C) U Map clusters show major populations. Immune showed similar clustering in post- treatment samples.</p>
<p>Supplementary Figure 5. Cell Density Analyses. Comparison of defined cell populations in pre- and post-treatment tumor specimens via CODEX. (A) Each indicated type is shown as a percentage total cells each post-tumor specimen for all five patients. (B) density T subpopulations preand patient. CD4 are different shades blue; CD8 red. was determined by count per microm2. increased substantially patient #5. (C) changes paired posttreatment samples. Patient #5 highlighted with blue...
Radiation therapy may enhance anti-tumor immune responses by several mechanisms including induction of immunogenic cell death. We performed a phase 2 study pembrolizumab with re-irradiation in patients recurrent glioblastoma.