A5100715334- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- RNA and protein synthesis mechanisms
- Metallurgical Processes and Thermodynamics
- Insect and Pesticide Research
- Genetically Modified Organisms Research
- Retinal Development and Disorders
- Metal Extraction and Bioleaching
- Iron and Steelmaking Processes
- Advanced biosensing and bioanalysis techniques
- Minerals Flotation and Separation Techniques
- Virus-based gene therapy research
- RNA regulation and disease
- Entomopathogenic Microorganisms in Pest Control
- HIV/AIDS drug development and treatment
- Biofuel production and bioconversion
- HIV Research and Treatment
- DNA and Nucleic Acid Chemistry
- Insect-Plant Interactions and Control
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Immunodeficiency and Autoimmune Disorders
- DNA Repair Mechanisms
- Cytokine Signaling Pathways and Interactions
- Enzyme Production and Characterization
Sichuan University
2022-2025
West China Hospital of Sichuan University
2022-2025
Army Medical University
2025
Xinqiao Hospital
2025
Biogas Institute of Ministry of Agriculture
2021-2024
Ministry of Agriculture and Rural Affairs
2021-2024
Chinese Academy of Agricultural Sciences
2024
Zhejiang Institute of Special Equipment Inspection
2024
Panzhihua University
2023-2024
Fujian Medical University
2024
SIRT3 is a major mitochondrial NAD(+)-dependent protein deacetylase playing important roles in regulating metabolism and energy production has been linked to the beneficial effects of exercise caloric restriction. emerging as potential therapeutic target treat metabolic neurological diseases. We report first sets crystal structures human SIRT3, an apo-structure with no substrate, structure peptide containing acetyl lysine its natural substrate acetyl-CoA synthetase 2, reaction intermediate...
Cytidine base editors (CBEs) and adenine (ABEs), composed of a cytidine deaminase or an evolved fused to Cas9 nickase, enable the conversion C·G T·A A·T G·C pair in organisms, respectively. Here, we show that BE3 ABE7.10 systems can achieve targeted mutation efficiency 53-88% 44-100%, respectively, both blastocysts Founder (F0) rabbits. Meanwhile, this strategy be used precisely mimic human pathologies by efficiently inducing nonsense missense mutations as well RNA mis-splicing rabbit. In...
Abstract CRISPR/Cas9 has been widely used in generating site-specific genetically modified animal models. Myostatin (MSTN) is a negative regulator of muscle mass, related to growth and differentiation. The knockout MSTN with the desired phenotype double successfully generated mice, goats, pigs cattle, but not rabbits. In this study, (KO) rabbits were by co-injection Cas9 mRNA sgRNA into zygotes. typical hyperplasia or hypertrophy fiber was observed KO Furthermore, similar found F1...
The evolution of insecticide resistance is a global constraint to agricultural production. Spinosad new, low-environmental-risk that primarily targets nicotinic acetylcholine receptors (nAChR) and effective against wide range pest species. However, after only few years application, field evolved emerged in the diamondback moth, Plutella xylostella, an important brassica crops worldwide. Hawaiian population results from single incompletely recessive autosomal gene, here we use AFLP linkage...
The ecological safety of transgenic insecticidal plants expressing crystal proteins (Cry toxins) from the bacterium Bacillus thuringiensis (Bt) continues to be debated. Much debate has focused on nontarget organisms, especially predators and parasitoids that help control populations pest insects in many crops. Although studies have been conducted predators, few reports examined but some them reported negative impacts. None previous were able clearly characterize cause impact. In order...
Cataracts are the leading cause of vision loss in world, although surgical treatment can restore cataract patients. Until now, there have been no adequate animal models for vivo studies artificial lens safety and drug interactions. Genetic demonstrated that GJA8 is involved maintaining opacity proper development. In this study, a model with gene knockout was developed via co-injection Cas9/sgRNA mRNA into rabbit zygotes. Our results showed mutation efficiency locus reached 98.7% embryos 100%...
CRISPR-Cas9 and base editors (BEs) systems are poised to become the gene-editing tool of choice in clinical contexts; however, large-fragment deletion was found Cas9-mediated mutation cells mice. In this study, by analyzing 16 gene-edited rabbit lines (including 112 rabbits) generated using SpCas9, BEs, xCas9, xCas9-BEs with long-range PCR genotyping long-read sequencing PacBio platform, we show extension thousands fragment deletions single-guide RNA/Cas9 xCas9 system rabbits, but no were...
Cytidine base editors (CBEs), composed of a cytidine deaminase fused to Cas9 nickase (nCas9), enable efficient C-to-T conversion in various organisms. However, current can induce unwanted bystander conversions when multiple Cs are present the ~ 5-nucleotide activity window deaminase, which negatively affects their precision. Here, we develop new editor significantly reduces activities.We used an engineered human APOBEC3G (eA3G) C-terminal catalytic domain with preferential cytidine-deaminase...
Tay-Sachs disease (TSD) is a progressive neurodegenerative disorder due to an autosomal recessively inherited deficiency of β-hexosaminidase A (HexA) 1 .The four-bases (TATC) insertion in exon 11 the HEXA (HEXA ins TATC) accounts for 80% from Ashkenazi Jewish population 2 .However, no typical clinical phenotypes, such as neurological abnormalities, restricted pattern distribution GM2-ganglioside and membranous cytoplasmic bodies brain, were observed -/-mouse models, difference ganglioside...
Bruton's tyrosine kinase is intimately involved in signal transduction pathways regulating survival, activation, proliferation, and differentiation of B lineage lymphoid cells. Mutations the human <i>btk</i> gene are cause X-linked agammaglobulinemia, a male immune deficiency disorder characterized by lack mature, immunoglobulin-producing lymphocytes. We have determined x-ray crystal structure domain its unphosphorylated state to 2.1 Å resolution. A comparison with structures other kinases...
In previous studies, CRISPR/Cas9 was shown to induce unexpected exon skipping; however, the mechanism by which this phenomenon is triggered controversial. By analyzing 22 gene-edited rabbit lines generated using CRISPR/Cas9, we provide evidence of skipping at high frequency in premature termination codon-mutated rabbits but not with a codon mutation 1 non-frameshift or missense mutations. Our results suggest that CRISPR-mediated depends on mutation-induced nonsense-associated altered splicing.
There is a continuing need to express new insect control compounds in transgenic maize against western corn rootworm, Diabrotica virgifera (LeConte) (WCR). In this study three experiments were conducted determine cross-resistance between the insecticidal DvSnf7 dsRNA, and Bacillus thuringiensis (Bt) Cry3Bb1; used WCR since 2003, with field-evolved resistance being reported. Laboratory susceptible Cry3Bb1-resistant evaluated dsRNA larval diet-incorporation bioassays. Additionally,...
Abstract Cytidine base editors, composed of a cytidine deaminase fused to Cas9 nickase, enable efficient C-to-T conversion in various organisms. However, current editors suffer from severe trade-off between editing efficiency and precision. Here, based on rationally mutated domain, we develop new editor, YFE-BE4max, effectively narrow the width as little approximately three nucleotides while maintaining high rabbits. Moreover, YFE-BE4max successfully mediated Tyr p. Q68Stop Lmna G607G...