A5028807813- Single-cell and spatial transcriptomics
- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Cell death mechanisms and regulation
- Cell Image Analysis Techniques
- Collagen: Extraction and Characterization
- Periodontal Regeneration and Treatments
- PARP inhibition in cancer therapy
- Bone Tissue Engineering Materials
- Cell Adhesion Molecules Research
Walter and Eliza Hall Institute of Medical Research
2023-2025
The University of Melbourne
2023-2024
Spatial transcriptomics allows gene expression to be measured within complex tissue contexts. Among the array of spatial capture technologies available is 10x Genomics' Visium platform, a popular method which enables transcriptome-wide profiling sections. offers range sample handling and library construction methods introduces need for benchmarking compare data quality assess how well technology can recover expected features biological signatures. Here we present SpatialBenchVisium, unique...
Background Spatial transcriptomics allows gene expression to be measured within complex tissue contexts. Among the array of spatial capture technologies available is 10x Genomics’ Visium platform, a popular method which enables transcriptomewide profiling sections. offers range sample handling and library construction methods introduces need for benchmarking compare data quality assess how well technology can recover expected features biological signatures. Results Here we present...
Spatial transcriptomics technology has developed rapidly in recent years, with various sequencing-based platforms such as 10x Visium, Slide-seq and Stereo-seq becoming widely used by researchers. Each platform brings its own set of protocols customised data analysis pipelines which presents challenges when the goal is to obtain uniformly preprocessed that conveniently formatted for downstream analysis. To address need simpler, open-source solutions deal spatial (sST) from different...
Abstract Necroptosis is a lytic form of regulated cell death reported to contribute inflammatory diseases the gut, skin and lung, as well ischemic-reperfusion injuries kidney, heart brain. However, precise identification cells tissues that undergo necroptotic in vivo has proven challenging absence robust protocols for immunohistochemical detection. Here, we provide automated immunohistochemistry detect core necroptosis regulators – Caspase-8, RIPK1, RIPK3 MLKL formalin-fixed mouse human...