A5058159877- Microbial Metabolic Engineering and Bioproduction
- Dental materials and restorations
- Endodontics and Root Canal Treatments
- Polyamine Metabolism and Applications
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Dental Radiography and Imaging
- Dental Trauma and Treatments
- Enzyme Catalysis and Immobilization
- Toxic Organic Pollutants Impact
- Computational Drug Discovery Methods
- Mass Spectrometry Techniques and Applications
- Microbial bioremediation and biosurfactants
- Nanomaterials for catalytic reactions
- Microbial Natural Products and Biosynthesis
- Viral Infectious Diseases and Gene Expression in Insects
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Advanced Photocatalysis Techniques
- Catalytic Processes in Materials Science
- Dental Implant Techniques and Outcomes
University of Manchester
2015-2024
The microbial production of fine chemicals provides a promising biosustainable manufacturing solution that has led to the successful growing catalog natural products and high-value chemicals. However, development at industrial levels been hindered by large resource investments required. Here we present an integrated Design-Build-Test-Learn (DBTL) pipeline for discovery optimization biosynthetic pathways, which is designed be compound agnostic automated throughout. We initially applied...
The field of synthetic biology aims to make the design biological systems predictable, shrinking huge space practical numbers for testing. When designing microbial cell factories, most optimization efforts have focused on enzyme and strain selection/engineering, pathway regulation, process development. In silico tools predictive bacterial ribosome binding sites (RBSs) RBS libraries now allow translational tuning biochemical pathways; however, methods predicting optimal combinations in...
Glucose is a key intermediate in cellulose photoreforming for H2 production. This work presents mechanistic investigation of glucose over TiO2 and Pt/m-TiO2 catalysts. Analysis the intermediates formed process confirmed α-scission mechanism oxidation forming arabinose (Cn-1 sugar) formic acid initial step. The selectivity to sugar products differed Pt/TiO2 TiO2, with showing lower due enhanced adsorption/conversion Pt/TiO2. In situ ATR-IR spectroscopy showed presence molecular formate on...
S-adenosyl-l-methionine (SAM)-dependent methyltransferases (MTs) catalyse the methylation of a vast array small metabolites and biomacromolecules. Recently, rare carboxymethylation pathways have been discovered, including carboxymethyltransferase enzymes that utilise carboxy-SAM (cxSAM) cofactor generated from SAM by cxSAM synthase (CmoA). We show how MT can to tetrahydroisoquinoline (THIQ) catechol substrates. Site-directed mutagenesis was used create orthogonal MTs possessing improved...
The rapid prototyping and optimization of plasmid-based recombinant gene expression is one the key steps in development bioengineered bacterial systems. Often, multiple genes or modules need to be coexpressed, for this purpose compatible, inducible plasmid systems have been developed. However, are not favored industrial processes, due their prohibitive cost, consequently conversion constitutive often desired. Here we present a set constitutive-expression plasmids purpose, which were...
Abstract S ‐adenosyl‐ l ‐methionine (SAM)‐dependent methyltransferases (MTs) catalyse the methylation of a vast array small metabolites and biomacromolecules. Recently, rare carboxymethylation pathways have been discovered, including carboxymethyltransferase enzymes that utilise carboxy‐SAM (cxSAM) cofactor generated from SAM by cxSAM synthase (CmoA). We show how MT can to tetrahydroisoquinoline (THIQ) catechol substrates. Site‐directed mutagenesis was used create orthogonal MTs possessing...
To determine the performance of a sophorolipid biosurfactant production process, it is important to have accurate and specific analytical techniques in place. Among most popular are anthrone assay, gravimetric quantification (hexane:ethyl acetate extraction), high-performance liquid chromatography (HPLC). The choice tool varies depending on cost, availability, ease use; however, these never been compared directly against one another. In this work, 75 fermentation broths with varying...