A5087459287- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Probiotics and Fermented Foods
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Escherichia coli research studies
- Gut microbiota and health
- Microbial Metabolites in Food Biotechnology
- CRISPR and Genetic Engineering
- Meat and Animal Product Quality
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolism and Applications
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Antibiotic Resistance in Bacteria
- Enzyme Production and Characterization
Université de Toulouse
2010-2024
Centre National de la Recherche Scientifique
2009-2024
Université Toulouse III - Paul Sabatier
2010-2024
Laboratoire de Microbiologie et Génétique Moléculaires
1997-2018
Background The population structure and diversity of Lactococcus lactis subsp. lactis, a major industrial bacterium involved in milk fermentation, was determined at both gene genome level. Seventy-six lactococcal isolates various origins were studied by different genotyping methods thirty-six strains displaying unique macrorestriction fingerprints analyzed new multilocus sequence typing (MLST) scheme. This gene-based analysis compared to genomic characteristics pulsed-field gel...
Temperate phage mv4 integrates its DNA into the chromosome of Lactobacillus delbrueckii subsp. bulgaricus strains via site-specific recombination. Nucleotide sequencing a 2.2-kb attP-containing fragment revealed presence four open reading frames. The larger frame, close to attP site, encoded 427-amino-acid polypeptide with similarity in C-terminal domain recombinases integrase family. Comparison sequences attP, bacterial attachment site attB, and host-phage junctions attL attR identified...
Lactococcus lactis subsp. strain A12 was isolated from sourdough. Combined genomic, transcriptomic, and phenotypic analyses were performed to understand its survival capacity in the complex sourdough ecosystem role microbial community. The genome sequence comparison of with IL1403 (a derivative an industrial dairy strain) revealed 78 strain-specific regions representing 23% total size. Most genes involved carbohydrate metabolism are potentially required for persistence Phenotype microarray,...
Bacteriophage mv4 is a temperate phage infecting Lactobacillus delbrueckii subsp. bulgaricus. During lysogenization, the integrates its genome into host chromosome at 3' end of tRNA(Ser) gene through site-specific recombination process (L. Dupont et al., J. Bacteriol., 177:586-595, 1995). A nonreplicative vector (pMC1) based on integrative elements (attP site and integrase-coding int gene) able to integrate wide range bacterial hosts, including plantarum, casei (two strains), Lactococcus...
Bacteria use the global bipolarization of their chromosomes into replichores to control dynamics and segregation genome during cell cycle. This involves protein activities by recognition specific short DNA motifs whose orientation along chromosome is highly skewed. The KOPS act in orienting activity FtsK translocase towards terminal replichore junction. have been identified γ-Proteobacteria Bacillus subtilis as closely related G-rich octamers. We motif Lactococcus lactis , a model bacteria...
Significance Toxin-antitoxin (TA) systems are small genetic modules known to modulate bacterial growth in response stress. Their major contribution the formation of persister cells and virulence several important pathogens has highlighted them as promising new targets for therapy. TA-chaperone (TAC) atypical TA tightly controlled by a third partner: molecular chaperone that directly assists antitoxin. Remarkably, TAC chaperones belong family canonical SecB facilitate protein secretion...
Abstract Through their involvement in the integration and excision of a large number mobile genetic elements, such as phages integrative conjugative elements (ICEs), site‐specific recombination systems based on heterobivalent tyrosine recombinases play major role genome dynamics evolution. However, despite hundreds these having been identified databases, very few have described detail, with none from that infect Bacillota (formerly Firmicutes ). In this study, we reanalyzed module...
The integrase of the temperate bacteriophage mv4 catalyzes site-specific recombination between phage attP site and host attB during Lactobacillus delbrueckii lysogenization. prophage is excised induction lytic growth. Excisive attR attL sites also catalyzed by phage-encoded recombinase, but directionality determined a second protein, factor (RDF). We have identified functionally characterized RDF involved in excision genome. RDF, (mv4)Xis, encoded gene early operon. It basic protein 56 amino...
ABSTRACT The temperate phage mv4 integrates its genome into the chromosome of Lactobacillus delbrueckii subsp. bulgaricus by site-specific recombination within 3′ end a tRNA Ser gene. Recombination is catalyzed phage-encoded integrase and occurs between attP site bacterial attB site. In this study, we show that functions in vivo Escherichia coli characterize with test involving compatible plasmids carrying sites. importance particular nucleotides sequence was determined site-directed...
ABSTRACT We report here the complete genome sequence of Lactococcus lactis subsp. strain A12, a isolated from sourdough. The circular chromosome and four plasmids reveal genes involved in carbohydrate metabolism that are potentially required for persistence this such complex ecosystem.
A direct method to study essential genes is construct conditional knock-down mutants by replacement of their native promoter an inducible one. In Mycobacteria, gene with anhydrotetracycline one was successfully used but required a multi-step approach. this work, we describe cassette for the engineering mutant, which allows one-step targeted mycobacterial promoters anhydrotetracycline-inducible promoter. The functionality tested clpP and SecA1 Mycobacterium smegmatis.
ABSTRACT The contributions of the five mv4 Int- and two Xis arm-binding sites to spatial intasome organization bacteriophage were found not be equivalent. 8-bp overlap region was mapped left extremity core is directly flanked by P2 Int site. These results absence characteristic core-binding suggest that site determinant for integrase positioning recognition region.
ABSTRACT Bacteriophage mv4 is a temperate bacterial virus able to integrate its genome at the 3’ end of tRNA SER Lactobacillus delbrueckii subsp. bulgaricus chromosome through site-specific recombination. Previous investigations revealed that Int/ attP / attB recombination module was atypical compared conventional heterobivalent tyrosine recombinases, such as paradigmatic Lambdavirus lambda integrase, suggesting alternative mechanism. In vitro assays with random DNA libraries were used...