Mast cells and nerve growth factor (NGF) have both been reported to be involved in neuroimmune interactions tissue inflammation. In many peripheral tissues, mast interact with the innervating fibers. Changes behaviors of these elements occur after injury/inflammation. As such conditions are typically associated rapid cell activation NGF accumulation inflammatory exudates, we hypothesized that may capable producing NGF. Here report (i) mRNA is expressed adult rat peritoneal cells; (ii)...

Mast cells are multifunctional bone marrow-derived found in mucosal and connective tissues the nervous system, where they play important roles tissue inflammation neuroimmune interactions. Very little is known about endogenous molecules mechanisms capable of modulating mast cell activation. Palmitoylethanolamide, peripheral tissues, has been proposed to behave as a local autacoid downregulating activation inflammation. A cognate N-acylamide, anandamide, ethanolamide arachidonic acid, occurs...

The amino acid L-glutamate is a neurotransmitter that mediates fast neuronal excitation in majority of synapses the central nervous system. Glutamate stimulates both N-methyl-D-aspartate (NMDA) and non-NMDA receptors. While activation NMDA receptors has been implicated variety neurophysiologic processes, excessive receptor stimulation (excitotoxicity) thought to be primarily responsible for injury wide acute neurological disorders including hypoxia-ischemia, seizures, trauma. Very little...

The conjugation of inorganic materials with biological systems has attracted research interest, as a consequence the availability sol–gel process, operating under experimental conditions compatible biomolecules and whole cells. In Section 2, merits limitations alkoxide-based silica an immobilization material are presented discussed particular attention to confinement living cells cell clusters. Biosil technology is based on encapsulation by layer deposited surface using precursors in gas...

1. Ranitidine interacts with liver microsomes from rats pretreated different inducers of cytochrome P-450 to produce substrate difference optical spectra a peak at 426-429 nm and trough 390-400 nm. 2. Cytochrome reduced dithionite in the presence ranitidine produced 447 3. Ks values for interaction (not reduced), calculated double reciprocal plots, were range 1.4-2.8 mM. 4. The O-dealkylation 7-ethoxycoumarin p-nitroanisole was inhibited by inhibition mixed type. Kii Kis were: dealkylation,...

We here report the results of our investigations carried out on verbascoside, a phenylpropanoid glycoside known for its antioxidant, anti-inflammatory and photoprotective actions. Verbascoside was obtained from Buddleia davidii meristematic cells, in turn using sustainable biotechnology platform which employs an vitro plant cell culture technology. first investigated to assess behaviour active ingredient solution or finished preparations, view potential topical use, especially skin...

The use of CNS cultures for detection and quantification neuronotrophic activity in the has been analyzed. In particular development, i.e., neurotransmitter uptake characteristics, survival dopaminergic GABAergic neurons fetal mouse (E13)-dissociated mesencephalic cells cultured serum-free, hormone-supplemented medium have assessed as a function culture time cell density. At all times, more than 98% were classified on basis immunocytochemical criteria. Results indicate that increase density...

This paper analyzes the effects of exogenously supplied GM1 on development, i.e., specific neurotransmitter uptake capability and survival, dopaminergic neurons present in fetal mouse- dissociated mesencephalic cells. Exogenous GM1, but not asialo-GM1, sialic acid, or oligosaccharide chain enhances a time- concentration-dependent manner 3H-dopamine (increase apparent Vmax decrease Km value) long-term survival neurons. The behavior require presence cell-derived neuronotrophic influences...

Abstract The experimental strategy of adding monosialoganglioside GM 1 to a culture medium fetal chick dorsal root ganglia (DRG) was utilized as model system in which examine the potential role modulation neuronal cell responsiveness nerve growth factor (NGF). Data indicate that addition DRG explants or dissociated cells enhances NGF‐induced neurite outgrowth, complexity, and survival following NGF withdrawal. molecule apparently facilitates acquisition maintenance specific properties....

The most effective therapy of human prolactinomas is represented by dopamine D-2 receptor agonists; there is, however, a population nonresponder patients who require surgical intervention. In the present study, we report that totally resistant to pharmacological have high potential both growing in soft agar and forming tumors nude mice lack receptors for dopamine. These express nerve growth factor (NGF) are sensitive its differentiating activity. After exposure NGF 4 days, prolactinoma cells...

Nerve growth factor (NGF) is well characterized for its neurotrophic actions on peripheral sensory and sympathetic neurons central cholinergic of the basal forebrain. Recent evidence, however, has shown high levels NGF to be present in a variety biological fluids after inflammatory autoimmune responses, suggesting that mediator immune interactions. Increased serum have been reported both humans experimental animal models psychological physical stress, thus implicating neuroendocrine...

The previous results suggest that peroxisome proliferator-activated receptor-alpha (PPAR)-<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>α</mml:mi></mml:math>, an intracellular transcription factor activated by fatty acids, plays a role in control of inflammation. There is persuasive epidemiological and experimental evidence dietary polyphenols have anti-inflammatory activity. In this regard, it has been demonstrated verbascoside (VB) functions as radical scavenger reduces...

The glycosylated phenylpropanoid verbascoside isolated from cultured cells of the medicinal plant Syringa vulgaris (Oleaceae) has previously been characterized as an effective scavenger biologically active free radicals such hydroxyl, superoxide, and nitric oxide, a chelator redox transition metal ions (Fe2+, Fe3+, Cu2+, Ni2+), inhibitor lipid peroxidation. In present work, we have compared cytoprotective effects biotechnologically produced with two commercially available polyphenols (the...

Edelweiss ( Leontopodium alpinum Cass.) is traditionally employed in folk medicine as an anti-inflammatory remedy. In nature, the plant sparsely available and protected; therefore production of callus cultures was established. A concentrated ethanolic extract culture homogenate, with leontopodic acid representing<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mn>55</mml:mn><mml:mo>±</mml:mo><mml:mn>2</mml:mn></mml:mrow></mml:math>% total phenolic fraction...