A5019664521- Nematode management and characterization studies
- Genetics, Aging, and Longevity in Model Organisms
- Kruppel-like factors research
- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Insect Pest Control Strategies
- Genetic Syndromes and Imprinting
- Agronomic Practices and Intercropping Systems
- Genomics, phytochemicals, and oxidative stress
- Parasite Biology and Host Interactions
- Parasitic Diseases Research and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Parasites and Host Interactions
- Adipose Tissue and Metabolism
- Selenium in Biological Systems
- Plant Disease Management Techniques
- Insect and Pesticide Research
- Weed Control and Herbicide Applications
- Adipokines, Inflammation, and Metabolic Diseases
- Epigenetics and DNA Methylation
- Transgenic Plants and Applications
- Agricultural Practices and Plant Genetics
- Peroxisome Proliferator-Activated Receptors
- CRISPR and Genetic Engineering
- Insect symbiosis and bacterial influences
Gomal University
2025
University of Veterinary and Animal Sciences
2024
Rutgers, The State University of New Jersey
1997-2023
University of the Punjab
2023
New York Blood Center
2001-2011
Instituto de Investigaciones Biomédicas Sols-Morreale
1999
Bowdoin College
1990
Cysteine proteases play critical biological roles in both intracellular and extracellular processes. We characterizedCe-cpl-1, a Caenorhabditis elegans cathepsin L-like cysteine protease. RNA interference with Ce-cpl-1activity resulted embryonic lethality transient delayed growth of larvae to egg producing adults, suggesting an essential role forcpl-1 during embryogenesis, most likely post-embryonic development. Cpl-1 gene (Ce-cpl-1:lacZ) is widely expressed the intestine hypodermal cells...
Background Cathepsin-like enzymes have been identified as potential targets for drug or vaccine development in many parasites, their functions appear to be essential a variety of important biological processes within the host, such molting, cuticle remodeling, embryogenesis, feeding and immune evasion. Functional analysis Caenorhabditis elegans cathepsin L (Ce-cpl-1) Z (Ce-cpz-1) has established that both genes are required early with Ce-cpl-1 having role regulating part processing yolk...
Lipid metabolic disorder is a critical risk factor for syndrome, triggering debilitating diseases like obesity and diabetes. Both diabetes are the epicenter of important medical issues, representing major international public health threat. Accumulation fat in adipose tissue, muscles liver and/or defects their ability to metabolize fatty acids, results insulin resistance. This triggers an early pathogenesis type 2 (T2D). In mammals, lipid metabolism involves several organs, including brain,...
We have analyzed the expression and function of Cecpz-1, a Caenorhabditis elegans cathepsin Z-like cysteine protease gene, during development worm. The cpz-1 gene is expressed in various hypodermal cells all developmental stages specifically gonads pharynx adult worms. Disruption by RNA interference or cpz-1(ok497) deletion mutant suggests that has role molting pathways. presence native CPZ-1 protein hypodermis/cuticle larval along length worms, as well cyclic transcript development,...
Hypoxia-induced adipokine release has been attributed mainly to HIF-1α. Here we investigate the role of intracellular calcium and NF-kB in hypoxia-dependent leptin, VEGF, IL-6 hypoxia-induced inhibition adiponectin human adipocytes.We used imaging compare status preadipocytes adipocytes. We subjected both cell types hypoxic conditions measured adipokines induced by hypoxia presence absence HIF-1α inhibitor YC-1, NF-κB SN50 chelator BAPTA-AM.We demonstrate reduced oscillations increased...
Abstract Herein, a series of picolinaldehyde‐based materials ( DMeP‐TFMP ) were synthesized, via the decarboxylative Suzuki coupling reaction using readily accessible 2‐formyl‐6‐pyridinoic acid, to investigate their nonlinear optical (NLO) properties. Various spectroscopic techniques like UV–vis, FT‐IR, ¹H‐NMR, and ¹ 3 C‐NMR employed elucidate molecular structures synthesized chromophores. Further, density functional theory (DFT) time dependent (TD‐DFT) calculations at M06/6‐311G (d,p) level...
Background: Microbial contamination of drinking water is a major public health concern in Pakistan, contributing to the high prevalence waterborne diseases due inadequate sanitation, aging infrastructure, and limited use modern detection techniques. Existing literature lacks consolidated understanding pathogen prevalence, methodologies, their implications. Objective: This narrative review aimed evaluate microbial focusing on key pathogens (E. coli, Salmonella spp., H. pylori, etc.), methods...
Background: Dyslipidemia, abnormal levels of cholesterol & lipids, is a major cause heart disease and can vary widely due to gender, lifestyle, access healthcare, especially in remote areas. Objective: The primary objective this study was examine gender sociocultural differences Dyslipidemia among patients from South Waziristan, Khyber Pakhtunkhwa, Pakistan. Methods: A cross-sectional conducted 104 Waziristan. Lipid profiles including LDL, TC, TG, HDL were measured. Data statistically...
Silicon micromachining has been used to fabricate microprobes for injecting DNA into cells. Arrays of very sharp pyramidal points are etched on a silicon substrate. Pressing these culture cells, allows biologically active material cross the cell wall barrier. Using microprobes, injected plant (tobacco leaves) and animal (nematodes)
A heat-shock response is induced when cells are exposed to temperatures slightly higher than their optimal physiological temperature. This based on the synthesis of proteins encoded by genes. correlation between increased thermotolerance and production 70-kDa protein (hsp70) has been observed in many organisms. We tested this hypothesis transferring a Caenorhabditis elegans heat-inducible hsp70 A-encoding gene into entomopathogenic nematodes Heterorhabditis bacteriophora Hp88. Successful...
Rhesus (Rh) proteins share a conserved 12-transmembrane topology and specify family of putative CO(2) channels found in diverse species from microbes to human, but their functional essentiality physiological importance metazoans is unknown. To address this key issue analyze Rh-engaged physiologic processes, we sought explore model organisms with fewer Rh genes yet are tractable genetic manipulations. In article, describe the identification nematodes two homologues that highly similar human...
We demonstrate that a Caenorhabditis elegans Krüppel-like transcription factor is involved in fat regulation, cell death, and phagocytosis C. elegans. Suppression of klf-1 function by RNA interference (RNAi) results increased storage the intestine RNAi worm directly or indirectly causes germ cells to die. These dead are not engulfed phagocytosed worm. High-level expression Ce-klf-1 during larval development, as well its specific localization worm's intestine, supports direct role for...
The abnormalities caused by excess fat accumulation can result in pathological conditions which are linked to several interrelated diseases, such as cardiovascular disease and obesity. This set of conditions, known metabolic syndrome, is a global pandemic enormous medical, economic, social concern affecting significant portion the world's population. Although genetics, physiology environmental components play major role onset excessive accumulation, little about how or what extent each these...