A5055917949- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- T-cell and Retrovirus Studies
- Viral-associated cancers and disorders
- Influenza Virus Research Studies
- Plant Virus Research Studies
- RNA modifications and cancer
- Circular RNAs in diseases
- Peptidase Inhibition and Analysis
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Ubiquitin and proteasome pathways
- Carcinogens and Genotoxicity Assessment
- vaccines and immunoinformatics approaches
- Crystallization and Solubility Studies
- Pluripotent Stem Cells Research
- Viral Infectious Diseases and Gene Expression in Insects
- X-ray Diffraction in Crystallography
- Effects and risks of endocrine disrupting chemicals
- Cell Image Analysis Techniques
- 14-3-3 protein interactions
- Computational Drug Discovery Methods
- RNA and protein synthesis mechanisms
- Air Quality Monitoring and Forecasting
- Gene expression and cancer classification
Litron Laboratories (United States)
2024-2025
New York University
2016-2021
New chemical inhibitors of protein-protein interactions are needed to propel advances in molecular pharmacology. Peptoids peptidomimetic oligomers with the capability inhibit by mimicking protein secondary structure motifs. Here we report silico design a macrocycle primarily composed peptoid subunits that targets β-catenin:TCF interaction. The interaction plays critical role Wnt signaling pathway which is over-activated multiple cancers, including prostate cancer. Using Rosetta suite...
We present a novel small molecule antiviral chemotype that was identified by an unconventional cell-free protein synthesis and assembly-based phenotypic screen for modulation of viral capsid assembly. Activity PAV-431, representative compound from the series, has been validated against infectious viruses in multiple cell culture models all six families causing most respiratory diseases humans. In animals, this demonstrated efficacious porcine epidemic diarrhoea virus (a coronavirus)...
Abstract Background Long INterspersed Element-1 (LINE-1) is an autonomous retroelement able to “copy-and-paste” itself into new loci of the host genome through a process called retrotransposition. The LINE-1 bicistronic mRNA codes for two proteins, ORF1p, nucleic acid chaperone, and ORF2p, protein with endonuclease reverse transcriptase activity. Both proteins bind in cis are necessary While transcription usually repressed most healthy somatic cells plethora mechanisms, ORF1p expression has...
Abstract This article describes a range of high‐dimensional data visualization strategies that we have explored for their ability to complement machine learning algorithm predictions derived from MultiFlow® assay results. For this exercise, focused on seven biomarker responses resulting the exposure TK6 cells each 126 diverse chemicals over concentrations. Obviously, challenges associated with visualizing were further complicated whenever there was desire represent entire chemical set as...
Long Interspersed Nuclear Element-1 (LINE-1) is an autonomous retrotransposon that generates new genomic insertions through the retrotransposition of a RNA intermediate. Expression LINE-1 tightly repressed in most somatic tissues to prevent DNA damage and ensure integrity. However, reactivation has been documented cancer role protein expression become interest development, progression, adaptation many epithelial neoplasms, including prostate cancer. Here, we examined endogenous localization...
ABSTRACT In vitro new approach methodologies used to assess chemicals for biological effects are typically designed limit the amount of test article required and promote efficiencies such as compatibility with liquid handlers, so forth. This is certainly true in case genetic toxicology, where many methods have been continue be developed 96‐ or 384‐well plate processing mind. However, one recognized concern microwell plates that volatility substances and/or their metabolites degradation...
ABSTRACT This laboratory has reported that the combined use of In Vitro MicroFlow and MultiFlow assays provides information regarding chemicals' genotoxic mode action (MoA). an effort to go beyond MoA assessments, we incorporated a panel biological response modifiers elicit specific effects on assays' biomarker profiles. was done pursue our hypothesis such perturbation signatures would reveal clastogenic mechanisms molecular targets. For this proof‐of‐concept study, exposed TK6 cells 20...
The autonomous retroelement Long Interspersed Element-1 (LINE-1) mobilizes though a copy and paste mechanism using an RNA intermediate (retrotransposition). Throughout human evolution, around 500,000 LINE-1 sequences have accumulated in the genome. Most of these belong to ancestral subfamilies, including L1PA2-L1PA7, can no longer mobilize. Only small fraction sequences, approximately 80 100 copies belonging L1Hs subfamily, are complete still capable retrotransposition. While silenced most...
We present a novel small molecule antiviral chemotype that was identified by an unconventional cell-free protein synthesis and assembly-based phenotypic screen for modulation of viral capsid assembly. Activity PAV-431, representative compound from the series, has been validated against infectious virus in multiple cell culture models all six families viruses causing most respiratory disease humans. In animals this demonstrated efficacious Porcine Epidemic Diarrhea Virus (a coronavirus)...
Abstract Background We previously examined expression of Long Interspersed Element-1 (LINE-1) in a variety prostate cancer cells including hormone-dependent LNCaP cells. These studies demonstrated and sub-cellular localization LINE-1 proteins, ORF1p, with antibody 4H1, ORF2p, chA1-L1. Results Here we conduct immunoprecipitation/mass spectrometry analysis using chA1-L1 against ORF2p Our results indicate that antigens detected by the include transcriptional regulator BCLAF1. show recognizes...
ABSTRACT The SARS Coronavirus 2 (SARS-CoV-2) pandemic presents new scientific and scale-up challenges for diagnostic capabilities worldwide. gold standard SARS-CoV-2 infection is a reverse transcription/quantitative PCR (RT-qPCR) which targets the viral genome, an assay that has now been performed on millions of patient specimens worldwide regardless symptomatic status. Recently Zhang et al. suggested possibility N gene could integrate into host cell DNA through action LINE-1...
Abstract Background Long INterspersed Element-1 (LINE-1) is an autonomous retroelement able to “copy-and-paste” itself into new loci of the host genome through a process called retrotransposition. The LINE-1 bicistronic mRNA codes for two proteins, ORF1p, nucleic acid chaperone, and ORF2p, protein with endonuclease reverse transcriptase activity. Both proteins bind in cis are necessary While transcription usually repressed most healthy somatic cells plethora mechanisms, ORF1p expression has...