A5035928273- Muscle metabolism and nutrition
- Muscle Physiology and Disorders
- Exercise and Physiological Responses
- Nutrition and Health in Aging
- Vitamin D Research Studies
- Bone fractures and treatments
- Adipose Tissue and Metabolism
- Sports Performance and Training
- Genetics, Aging, and Longevity in Model Organisms
- Hormonal and reproductive studies
- GDF15 and Related Biomarkers
- Genetics and Physical Performance
- Bone health and osteoporosis research
- Adipokines, Inflammation, and Metabolic Diseases
- Bone Metabolism and Diseases
- Aging and Gerontology Research
- RNA modifications and cancer
- 3D Printing in Biomedical Research
- Muscle activation and electromyography studies
Nottingham Trent University
2022-2024
Örebro University
2017-2022
The Vitamin D receptor (VDR) has been positively associated with skeletal muscle mass, function and regeneration. Mechanistic studies have focused on the loss of receptor, in vivo whole-body knockout models demonstrating reduced myofibre size impaired development. To understand mechanistic role upregulation VDR elicits mass/health, we studied impact over-expression (OE) before exploring importance expression upon hypertrophy humans. Wistar rats underwent electrotransfer (IVE) to overexpress...
Abstract Background The andropause is associated with declines in serum testosterone (T), loss of muscle mass (sarcopenia), and frailty. Two major interventions purported to offset sarcopenia are anabolic steroid therapies resistance exercise training (RET). Nonetheless, the efficacy physiological molecular impacts T therapy adjuvant short‐term RET remain poorly defined. Methods Eighteen non‐hypogonadal healthy older men, 65–75 years, were assigned a random double‐blinded fashion receive,...
What is the central question of this study? Is electrical pulse stimulation (EPS) an in vitro exercise model able to elicit hypertrophy human muscle cells? main finding and its importance? The addition a restitution period 8 h after EPS induces enlargement cells, major physiological end-point resistance exercise. This supported by downregulation myostatin, negative regulator mass, increased phosphorylated mTOR 4E-BP1, key factors growth cascade. proof-of-concept study provides...
Key points Reduced vitamin D receptor (VDR) expression prompts skeletal muscle atrophy. Atrophy occurs through catabolic processes, namely the induction of autophagy, while anabolism remains unchanged. In response to VDR‐knockdown mitochondrial function and related gene‐set is impaired. vitro VDR knockdown induces myogenic dysregulation occurring impaired differentiation. These results highlight autonomous role has within mass regulation. Abstract Vitamin deficiency estimated affect ∼40%...
The skeletal muscle anabolic effects of n-3 polyunsaturated fatty acids (n-3 PUFA) appear favoured towards women; a property that could be exploited in older women who typically exhibit poor growth responses to resistance exercise training (RET). Here we sought generate novel insights into the efficacy and mechanisms PUFA alongside short-term RET women.We recruited 16 healthy (Placebo n = 8 (PLA): 67±1y, 8: 64±1y) randomised double-blind placebo-controlled trial PUFA; 3680 mg/day versus PLA)...
Sarcopenia is an age-related muscle disease that increases the risk of falls, disabilities, and death. It associated with increased protein degradation driven by molecular signalling pathways including Akt FOXO1. This study aims to identify genes, gene interactions, processes aging exercise in older adults remained undiscovered until now leveraging on artificial intelligence approach called neural network inference (ANNi).
Intermittent and continuous mechanical loads are known to influence osteogenic activity. The present study examines the effects of matched intermittent load in vitro on bone formation markers. MC3T3 (mouse pre-osteoblasts) were cultured placed a bioreactor undergo continuous, intermittent, or unloading for 1, 3 12 days. Loading conditions magnitude, duration frequency. Each time point was analysed alkaline phosphatase (ALP) activity, procollagen 1 N-terminal propeptide (PINP) alizarin red...
Abstract Ageing limits growth capacity of skeletal muscle (e.g. in response to resistance exercise), but the role satellite cell (SC) function driving this phenomenon is poorly defined. Younger (Y) (~ 23 years) and older (O) men 69 (normal-weight BMI) underwent 6 weeks unilateral exercise training (RET). Muscle biopsies were taken at baseline after 3-/6-week training. We determined size by fibre CSA (and type), SC number, myonuclei counts DNA synthesis (via D 2 O ingestion). At baseline,...
Intermittent and continuous mechanical loads are known to influence osteogenic activity. The present study examines the effects of matched intermittent load in vitro on bone formation markers. MC3T3 (mouse pre-osteoblasts) were cultured placed a bioreactor undergo continuous, intermittent, or unloading for 1, 3 12 days. Loading conditions magnitude, duration frequency. At each time point cells analysed alkaline phosphatase (ALP) activity, procollagen I N-terminal propeptide (PINP) alizarin...
Healthy skeletal muscle can regenerate after ischaemic, mechanical, or toxin-induced injury, but ageing impairs that regeneration potential. This has been largely attributed to dysfunctional satellite cells and reduced myogenic capacity. Understanding which signalling pathways are associated with myogenesis impaired provide valuable information about the mechanisms driving prompt development of new therapies. To investigate this, we developed a high-throughput in vitro model assess...
PURPOSE: To study systemic effects of sprint exercise combined with nutrient ingestion on muscle cell hypertrophy. It was hypothesized that the size human cells increases when they are exposed to post-exercise serum in but not placebo condition. Previously studies have shown oral essential amino acids (EAA) and carbohydrate results higher activation Akt/mTOR signalling rate protein synthesis following humans. Both local factors may contribute these effects. Moreover, If nutrient-induced...
While age-related elevations in systemic inflammation may contribute to the accelerated loss of skeletal muscle mass, previous findings have been based on a limited number biomarkers. Moreover, whether links between and mass are independent protein intake habitual physical activity (PA) remain unknown. PURPOSE: The aim study was explore inflammatory biomarkers older women with different metabolic risk status, while accounting for adherence guidelines PA. METHODS: Skeletal index (SMI)...
Skeletal muscle is a highly adaptive tissue that responds to different stimuli such as exercise and supplements. However, age-related changes in fibres can modify responses with negative impacts on health. Several genes molecular mechanisms have been identified, but the full array remains incomplete. PURPOSE: To identify most influenced associated (i) skeletal muscle; (ii) long term older adults (iii) supplement administration adults. METHODS: Six publicly available datasets were selected...
Healthy skeletal muscle can regenerate after ischemic, mechanical or toxin-induced injury. However, age-related changes in cells and their niche may impair the regeneration potential. While strategies to are being developed, vitro models investigate regenerative potential injury aged versus young lacking. PURPOSE: This study aims develop of using from older donors. METHODS: Differentiated C2C12 murine human myoblasts old donors were injured exposure 12% barium chloride for 6 h. Regeneration...