A5059171900- Angiogenesis and VEGF in Cancer
- GDF15 and Related Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Cardiovascular Disease and Adiposity
- Cardiac Imaging and Diagnostics
- Cardiovascular Health and Disease Prevention
- Insect Resistance and Genetics
- Cardiovascular Function and Risk Factors
- Nutrition and Health in Aging
- Lymphatic System and Diseases
- Cerebrovascular and Carotid Artery Diseases
- Enzyme Catalysis and Immobilization
- Rheumatoid Arthritis Research and Therapies
- Biomarkers in Disease Mechanisms
- T-cell and B-cell Immunology
- Atherosclerosis and Cardiovascular Diseases
- Cardiomyopathy and Myosin Studies
- Pericarditis and Cardiac Tamponade
- Heart Failure Treatment and Management
- Takotsubo Cardiomyopathy and Associated Phenomena
- Enzyme Structure and Function
- Nuclear Receptors and Signaling
- Genetically Modified Organisms Research
- Microbial Metabolic Engineering and Bioproduction
- Galectins and Cancer Biology
Kobe Medical Center
2011-2024
The University of Tokyo
1986-2024
Tokyo Institute of Technology
2024
National Hospital Organization
2009-2023
Nagasaki University Hospital
2023
Shimizu (Japan)
2001-2018
Sumitomo Seika Chemicals (Japan)
2010
Sumitomo Chemical (Japan)
1984-2009
Chiba University
2005
National Hospital
2000-2003
A human B cell subpopulation identifiable by the expression of surface antigen Leu-1 (CD5) is responsible for most immunoglobulin M rheumatoid factor secreted in vitro after cells are stimulated with Staphylococcus aureus . The ability bearing marker (Leu-1 + ) to secrete present early development and extends adulthood, since from cord blood peripheral lymphocytes both normal adults patients certain autoimmune conditions comparable amounts. neonatal enrichment cells, presence increased...
The partial amino acid sequence of the NH2 terminus a factor named human B-cell differentiation or stimulatory 2 (BSF-2) has been determined. Antibodies raised against synthetic peptide corresponding to residues 1-13 NH2-terminal specifically react with BSF-2 generated by T-cell line and phytohemagglutinin-stimulated normal T cells. Furthermore, antipeptide antibodies BSF-2-like produced cardiac myxoma as well uterine cervical carcinoma results show that functions in vivo suggest...
We constructed a full-length cDNA coding for 3-methylcholanthrene-inducible rat liver cytochrome P-450MC by the method of Okayama and Berg. The isolated clone pAU157 contained insert 2.7 kb in length. Sequence analysis revealed that amino acid sequence was composed 523 residues, including initial 22 N-terminal acids whose determined with purified protein. primary structure found to contain two highly conserved regions as pointed out from comparisons reported sequences P-450 species....
Despite substantial data on radioligand binding to the sigma receptor, neither physiologic function nor intracellular mechanism of this receptor is known. In study, we examined effect ligands Ca++ influx induced by N-methyl-D-aspartate (NMDA) in single primary cultured rat frontal cortical neurons with fluorescence video microscopy. All tested reduced NMDA-induced increase concentration ([Ca++]i) a dose-dependent manner IC50 values low micromolar range. Inhibition haloperidol and...
A 75-year-old woman developed left ventricular apical ballooning, shortly after recovering from status epileptics. Plasma noradrenaline and adrenaline levels were 2.05 ng/ml 0.48 ng/ml, respectively. Endomyocardial biopsy disclosed patchy areas of interstitial myocardial fibrosis, atrophy vacuolization cardiac myocytes, some disappearance myocyte nuclei. Follow-up echocardiography showed that the ballooning was restored to normal within 25 days. These findings are compatible with neurogenic...
For the first time, we performed an epidemiological study of SSc in Japan to factors influencing prognosis, survival rate and cause death Japanese patients compare our data with those from foreign countries. Prognosis 496 progressive systemic sclerosis (SSc) was analyzed based on clinical described case cards provided by members Scleroderma Research Committee Ministry Health Welfare. The essential observation period 5 20 years, at ending 1994. Ninety died (males 11, females 79). age onset...
An enzyme active against p -nitrophenyl-β- d -cellobioside was purified from Ruminococcus albus to homogeneity. The identified as a cellobiosidase.
Background The lymphatic system has been suggested to play an important role in cholesterol metabolism and cardiovascular disease. However, the relationships of vascular endothelial growth factor‐C ( VEGF ‐C), a central player lymphangiogenesis, with mortality events patients suspected or known coronary artery disease are unknown. Methods Results We performed multicenter, prospective cohort study 2418 undergoing elective angiography. primary predictor was serum levels ‐C. outcome all‐cause...
Background VEGF-D (vascular endothelial growth factor D) and VEGF-C are secreted glycoproteins that can induce lymphangiogenesis angiogenesis. They exhibit structural homology but have differential receptor binding regulatory mechanisms. We recently demonstrated the serum level is inversely independently associated with all-cause mortality in patients suspected or known coronary artery disease. investigated whether had distinct relationships cardiovascular events those patients. Methods...
The gene coding for a novel esterase which stereoselectively hydrolyzes the (+)-trans (1R,3R) stereoisomer of ethyl chrysanthemate was cloned from Arthrobacter globiformis SC-6-98-28 and overexpressed in Escherichia coli. cellular content active enzyme reached 33% total soluble protein recombinant E. coli JM105 cells 5.6 g/liter culture by high-density cell cultivation. hydrolytic activity 605 mumol chrysanthemic acid per min g dry cells, is approximately 2,500-fold higher than that A....
A soluble enzyme from quail oviduct which incorporates sulfate into position 6 of the nonreducing N-acetylgalactosamine 4-sulfate end group chondroitin has been purified.This (termed "terminal 6-sulfotransferase") was partially separated a 6-sulfotransferase present in same tissue catalyzes incorporation interior portion unsulfated chondroitin.The basic requirements for terminal reaction were shown to be 3'-phosphoadenylyl (donor) and (acceptor).The substitution (prepared squid skin)...
Abstract The modes of polymerization N‐substituted acrylamides catalyzed by sodium tert ‐butoxide at 105°C. were investigated means IR spectra. N‐cyclohexylacrylamide gave a vinyl polymer. All other monomers (N‐substituents benzyl, p ‐phenethyl, ‐anisyl, ‐tolyl, phenyl, and m ‐chlorophenyl) hydrogen‐migrated polymers, i.e. , poly‐N‐substituted‐β‐alanines. Thus electron‐releasing or ‐attracting effects the N‐substituents, acidities migrating amide‐hydrogens, might be responsible for choice...
Gradient-polyacrylamide-gel electrophoresis of human milk serum separated three high-Mr glycoprotein bands. The properties the components corresponding to bands (tentatively termed 'Components C, B and A' in their order migration) were compared by staining with four monoclonal antibodies lectins. Components C both reacted antibodies, but Component A did not. stained peanut (Arachis hypogaea) agglutinin (PNA) wheat (Triticum)-germ (WGA), being soya-bean (Glycine max) as well PNA WGA. These...
Background: The effects of the 3 classes L-type calcium-channel blockers (CCBs) on vascular endothelial function have not been clarified in patients with coronary vasospasm. Methods and Results: Twenty-five normotensive (age 64.0±1.4 years) vasospasm were randomly treated for months benidipine, diltiazem, verapamil, which belong to dihydropyridine, benzothiazepine, phenylalkylamine CCBs, respectively. Endothelium-dependent flow-mediated dilatation (FMD), endothelium-independent...