A5030345253- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Oral microbiology and periodontitis research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune cells in cancer
- Cancer Genomics and Diagnostics
- Oral and gingival health research
- Single-cell and spatial transcriptomics
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
- Cystic Fibrosis Research Advances
University at Buffalo, State University of New York
2016-2024
BioLegend (United States)
2024
Massachusetts General Hospital
2023
Buffalo State University
2023
Abstract Analysis of the human hematopoietic progenitor compartment is being transformed by single-cell multimodal approaches. Cellular indexing transcriptomes and epitopes sequencing (CITE-seq) enables coupled surface protein transcriptome profiling, thereby revealing genomic programs underlying states. To perform CITE-seq systematically on primary bone marrow cells, we used titrations with 266 antibodies (antibody-derived tags) machine learning to optimize a panel 132 antibodies....
ABSTRACT Candida auris is a newly identified species causing invasive candidemia and candidiasis. It has broad multidrug resistance (MDR) not observed for other pathogenic species. Histatin 5 (Hst 5) well-studied salivary cationic peptide with significant antifungal activity against albicans an attractive candidate treating MDR fungi, since antimicrobial peptides induce minimal drug resistance. We investigated the susceptibility of C. to Hst neutrophils, two first-line innate defenses in...
Candida albicans , the causative agent of mucosal infections, including oropharyngeal candidiasis (OPC), as well bloodstream is becoming increasingly resistant to existing treatment options. In absence novel drug candidates, repurposing aimed at using drugs treat off-label diseases a promising strategy.
Host phagocytic cells are crucial players in initial defense against Candida albicans infection. C. utilizes MAP kinases and Ras1 stress response signaling pathways to protect itself from killing by immune cells. In this study, we tested the importance of these phagocytosis neutrophils subsequent phagosomal survival. Phagocytosis was influenced morphology, so hyphal length >10 μm reduced index (PI) 2- 3-fold human neutrophils. Primary killed 81% phagocytosed albicans, while primary mouse 63%...
Candida albicans is the predominant fungus colonizing oral cavity that can have both synergistic and antagonistic interactions with other bacteria. Interkingdom polymicrobial associations modify fungal pathogenicity are believed to increase microbial resistance innate immunity. However, it not known how these alter survival during phagocytic killing. We demonstrated secreted molecules of S. gordonii P. aeruginosa C. within phagosome macrophages pathogenic phenotypes, including filamentation...
Abstract Oral mucosal colonization by C. albicans (Ca) is benign in healthy people but progresses to deeper infection known as oropharyngeal candidiasis (OPC) that may become disseminated when combined with immunosuppression. Cortisone-induced immunosuppression a well-known risk factor for OPC, however the mechanism which it permits poorly understood. Neutrophils are primary early sentinels preventing invasive fungal growth, and here we identify vivo neutrophil functional complexes swarms...
Abstract Oral mucosal colonization by Candida albicans is benign in healthy people but progresses to deeper infection, known as oropharyngeal candidiasis, that may become disseminated when combined with immunosuppression. Cortisone use and neutropenia are risk factors for invasive fungal infections; however, the mechanisms poorly understood. Here, we identify vivo neutrophil functional complexes swarms crucial preventing C. epithelial invasion. Anti-Ly6G antibody treatment impaired swarm...