A5029404167- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
- Receptor Mechanisms and Signaling
- Bacterial Genetics and Biotechnology
- Single-cell and spatial transcriptomics
- Nicotinic Acetylcholine Receptors Study
- HER2/EGFR in Cancer Research
- Advanced Biosensing Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Monoclonal and Polyclonal Antibodies Research
- Estrogen and related hormone effects
- Cell Image Analysis Techniques
- Reproductive System and Pregnancy
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- Glaucoma and retinal disorders
- Gene expression and cancer classification
- Bone Metabolism and Diseases
- Advanced biosensing and bioanalysis techniques
- Genetics and Neurodevelopmental Disorders
- Genomics and Phylogenetic Studies
Miltenyi Biotec (Germany)
2023-2024
Yale University
2007-2008
Enzo Life Sciences (United States)
2006
University of Vermont
1999-2004
Université de Montréal
1993-2001
Laboratoire de Neurosciences Cognitives
1996
Centre National de la Recherche Scientifique
1989-1993
Abstract Background Whole genome amplification is an increasingly common technique through which minute amounts of DNA can be multiplied to generate quantities suitable for genetic testing and analysis. Questions amplification-induced error template bias generated by these methods have previously been addressed either small scale (SNPs) or large (CGH array, FISH) methodologies. Here we utilized whole sequencing assess in both coding non-coding regions two bacterial genomes. Halobacterium...
Identification of common molecular pathways affected by genetic variation in autism is important for understanding disease pathogenesis and devising effective therapies. Here, we test the hypothesis that rare metabotropic glutamate-receptor (mGluR) signaling pathway contributes to susceptibility. Single-nucleotide variants genes encoding components mGluR were identified high-throughput multiplex sequencing pooled samples from 290 non-syndromic cases 300 ethnically matched controls on two...
Abstract Context.—There is critical need for standardization of HER2 immunohistochemistry testing in the clinical laboratory setting. Recently, American Society Clinical Oncology and College Pathologists have submitted guidelines recommending that laboratories achieve 95% concordance between assays observers testing. Objective.—As a potential aid to pathologists achieving these new guidelines, we conducted an examination using automated quantitative analysis (AQUA analysis) provide...
Abstract Context.—There is critical need for standardization of HER2 immunohistochemistry testing in the clinical laboratory setting. Recently, American Society Clinical Oncology and College Pathologists have submitted guidelines recommending that laboratories achieve 95% concordance between assays observers testing. Objective.—As a potential aid to pathologists achieving these new guidelines, we conducted an examination using automated quantitative analysis (AQUA analysis) provide...
Inherent to most tissue image analysis routines are user-defined steps whereby specific pixel intensity thresholds must be set manually differentiate background from signal-specific pixels within multiple images. To reduce operator time, remove operator-to-operator variability, and obtain objective optimal separation for each image, we have developed an unsupervised pixel-based clustering algorithm allowing the differentiation of signal background, compartment-specific on image-by-image...
To form a catalytically active complex, the essential nucleotides of hairpin ribozyme, embedded within internal loops two domains, must interact with one another. Little is known about nature these interdomain interactions. In work presented here, we have used recent topographical constraints and other biochemical data in conjunction molecular modeling (constraint-satisfaction program MC-SYM) to generate testable models Visual analysis generated has revealed potential base pair between...
Single mutations at the end of 5′ proximal helix and in 915 region (13UåA or C; 914AåU G), double 914Aå U; 13UåC 914AåG) were constructed into Escherichia coli 16S ribosomal RNA. The introduced an expression plasmid containing rrnB operon under transcriptional control temperature-inducible λPL promoter. None mutant rRNAs affected cell growth when expressed. Ribosomes extracted after induction assayed for their capacity to bind error-inducing drug streptomycin translational misreading...
Previous works have shown the potential location of 5-HT1B binding sites on retinal afferents to superior colliculus in rat. In order confirm this hypothesis, distribution sites, labelled by S-CM-G[125I]TNH2, was analysed quantitative autoradiography at both light and electron microscopic levels upper stratum griseum superficiale (SGS) colliculus, 5 days after unilateral eye ablation. At level, no were found be associated with capillaries, glial, or neuronal cell bodies, but fine neuropile...
Abstract Spatial Biology has evolved from the molecular characterization of microdissected cells to high throughput spatial RNA and protein expression analysis at scale. The main limitation technologies so far is inability resolve information in same histological section. Here, we report for first time integration highly multiplexed detection on tissue We developed a new, automated, method (RNAsky™), which based targeted rolling circle amplification iterative staining. combine RNAsky with...
Journal Article Positions 13 and 914 in Escherichia coli 16S ribosomal RNA are involved the control of translational accuracy Get access Robert Pinard, Pinard Departement de Biochimie, Université MontréalMontreal, Quebec H3C 3J7, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Marc Côté, Côté Catherine Payant, Payant Léa Brakier-Gingras * *To whom correspondence should be addressed Nucleic Acids Research, Volume 22, Issue 4, 25 February 1994, Pages...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMutations at Positions 13 and/or 914 in Escherichia coli 16 S Ribosomal RNA Interfere with the Initiation of Protein SynthesisRobert Pinard, Catherine Payant, and Lea Brakier-GingrasCite this: Biochemistry 1995, 34, 29, 9611–9616Publication Date (Print):July 25, 1995Publication History Published online1 May 2002Published inissue 25 July 1995https://pubs.acs.org/doi/10.1021/bi00029a038https://doi.org/10.1021/bi00029a038research-articleACS...
Within the hairpin ribozyme, structural elements required for formation of active tertiary structure are localized in two independently folding domains, each consisting an internal loop flanked by helical elements. Here, we present results a systematic examination relationship between and ability RNA to form catalytically structure. Deletions mutational analyses indicate that helix 1 (H1) domain A can be entirely eliminated, while segments helices 2, 3, 4 also deleted. From these results,...