A5051554901- Silk-based biomaterials and applications
- Genomics and Phylogenetic Studies
- PI3K/AKT/mTOR signaling in cancer
- Plant Gene Expression Analysis
- Fungal Biology and Applications
- Fungal and yeast genetics research
- Ichthyology and Marine Biology
- Biochemical and Structural Characterization
- RNA and protein synthesis mechanisms
- Plant biochemistry and biosynthesis
- Parasite Biology and Host Interactions
- RNA Interference and Gene Delivery
- Marine Ecology and Invasive Species
- Antimicrobial Peptides and Activities
- Advanced biosensing and bioanalysis techniques
- Polymer Surface Interaction Studies
- Microbial Natural Products and Biosynthesis
- Cancer-related molecular mechanisms research
- Effects and risks of endocrine disrupting chemicals
- Polysaccharides and Plant Cell Walls
- Bacteriophages and microbial interactions
- Wound Healing and Treatments
- 3D Printing in Biomedical Research
- Dermatologic Treatments and Research
- Bioeconomy and Sustainability Development
Agency for Science, Technology and Research
2013-2023
Institute of Molecular and Cell Biology
2021-2023
Institute of Bioengineering and Nanotechnology
2020
Star Technology and Research (United States)
2018
The emergence of jawed vertebrates (gnathostomes) from jawless was accompanied by major morphological and physiological innovations, such as hinged jaws, paired fins immunoglobulin-based adaptive immunity. Gnathostomes subsequently diverged into two groups, the cartilaginous fishes bony vertebrates. Here we report whole-genome analysis a fish, elephant shark (Callorhinchus milii). We find that C. milii genome is slowest evolving all known vertebrates, including 'living fossil' coelacanth,...
The sucker ring teeth (SRT) of Humboldt squid exhibit mechanical properties that rival those robust engineered synthetic polymers. Remarkably, these are achieved without a mineral phase or covalent cross-links. Instead, SRT exclusively made silk-like proteins called "suckerins", which assemble into nanoconfined β-sheet reinforced supramolecular networks. In this study, three streamlined strategies for full-length recombinant suckerin protein production and purification were developed....
Target of Rapamycin Complex 1 (TORC1) is a highly conserved eukaryotic protein complex that couples the presence growth factors and nutrients in environment with cellular proliferation. TORC1 primarily implicated linking amino acid levels yeast mammals. Although glucose deprivation has been shown to cause inactivation yeast, precise role signaling underlying mechanisms remain unclear.We demonstrate medium both necessary sufficient for activation. activity increases upon addition cells...
The solution structure of a suckerin protein obtained by NMR illustrates β-sheet conformation with stabilising aromatic interactions in dynamic domains.
Unbiased metagenomic sequencing is conceptually well-suited for first-line diagnosis as all known and unknown infectious entities can be detected, but costs, turnaround time human background reads in complex biofluids, such plasma, hinder widespread deployment. Separate preparations of DNA RNA also increases costs. In this study, we developed a rapid unbiased metagenomics next-generation (mNGS) workflow with depletion method (HostEL) combined DNA/RNA library preparation kit (AmpRE) to...
In this study, we report antifungal activity of auroramycin against Candida albicans, tropicalis, and Cryptococcus neoformans. Auroramycin, a potent antimicrobial doubly glycosylated 24-membered polyene macrolactam, was previously isolated characterized, following CRISPR-Cas9 mediated activation silent polyketide synthase biosynthetic gene cluster in Streptomyces rosesporous NRRL 15998. Chemogenomic profiling yeast has linked its bioactivity to vacuolar transport membrane organization. This...
Development of DNA aptamer screens that are both simple and informative can increase the success rate selection induce greater adoption. High eIF4e levels contribute to malignancies, thus presents itself as a valuable target for aptamer-based inhibition screen. Here, we demonstrate method rapid looped aptamers against by combining negative purification in single step, followed characterization with high throughput sequencing. The resulting show functional binding inhibit translation...
Mirtrons are introns that form pre-miRNA hairpins after splicing to produce RNA interference (RNAi) effectors distinct from Drosha-dependent intronic miRNAs, and will be especially useful for co-delivery of coding genes RNAi. A specific family mirtrons – 3′-tailed has precisely defined on the 5′ end by splice site 3′ branch point. Here, we present design principles artificial demonstrate, first time, efficient gene knockdown with tailed within eGFP region. These mirtrons, unlike canonical...
Abstract We have isolated Hypoculoside, a new glycosidic amino alcohol lipid from the fungus Acremonium sp. F2434 belonging to order Hypocreales and determined its structure by 2D-NMR (Nuclear Magnetic Resonance) spectroscopy. Hypoculoside has antifungal, antibacterial cytotoxic activities. Homozygous profiling (HOP) of hypoculoside in Saccharomyces cerevisiae (budding yeast) revealed that several mutants defective vesicular trafficking vacuolar protein transport are sensitive hypoculoside....
Abstract Phthalates are ubiquitously used as plasticizers in various consumer care products. Diethyl phthalate (DEP), one of the main phthalates, elicits developmental and reproductive toxicities but underlying mechanisms not fully understood. Chemogenomic profiling DEP S. cerevisiae revealed that two transcription factors Stp1 Dal81 involved Ssy1-Ptr5-Ssy5 (SPS) amino acid-sensing pathway provide resistance to DEP. Growth inhibition yeast cells by was stronger poor nitrogen medium...
The exact mechanisms underlying hypertrophic scarring is yet to be fully understood. However, excessive collagen deposition by fibroblasts has been demonstrated result in scar formation, and synthesis dermal regulated the transforming growth factor‑β1/Smad signaling pathway. In view of this, a Smad‑binding decoy was designed its effects on scar‑derived human skin evaluated. results present study revealed that Smad attenuates total amount collagen, I Smad2/3 expression fibroblasts. Data from...
The standard of care for thyroid cancer management is thyroidectomy and adjuvant radioactive iodine (RAI). There a paucity clinical tool that quantifies residual volume reliably precise RAI dosing. Serum thyroglobulin (TG), tumour marker cancer, takes 4 weeks complete clearance due to its long half-life, might be undetectable in 12% structural disease patients. It detects recurrence with sensitivity 19-40%, mainly attributed issue TG antibody interference immunometric assay. We hypothesise...
Abstract Unbiased metagenomic sequencing is conceptually well-suited for first-line infectious disease diagnostics because it does not require guesswork on the causative organism and can cover all known unknown entities other than prions. However, costs, turnaround time human background reads in complex biofluids such as plasma stand way of more widespread deployment. Many protocols also separate library preparations DNA RNA analytes, increasing costs detection. In this study, we developed a...
Nanoscopic structural control with long-range ordering remains a profound challenge in nanomaterial fabrication. The nanoarchitectured egg cases of elasmobranchs rely on hierarchically ordered latticework for their protective function─serving as an exemplary system nanoscale self-assembly. Although the proteinaceous precursors are known to undergo intermediate liquid crystalline phase transitions before being structurally arrested final nanolattice architecture, sequences have so far...
Bariatric surgery results in sustained weight loss and improvement glucose homeostasis. However, the lack of accessible non-invasive tools to examine molecular alterations occurring pancreas limits our understanding causes recovery Here, we describe use a circulating cell free mRNA (cfmRNA) based multiplex qPCR assay selectively amplify quantify pancreatic specific transcripts levels within plasma. We applied this cohort 58 plasma samples consisting 10 patients that tracks multiple time...
The novel coronavirus disease 2019 (COVID-19) pandemic poses a serious public health risk. In this report, we present modified sequencing workflow using short tiling (280bp) amplicons library preparation method paired with Illumina’s iSeq100 desktop sequencer. We demonstrated the utility of our in identifying gapped reads that capture characteristics subgenomic RNA junctions within patient cohort. These analytical and approaches allow versatile, small footprint decentralized deployment can...
Abstract Target of Rapamycin Complex 1 (TORC1) is a highly conserved eukaryotic protein complex that couples the presence growth factors and nutrients in environment with cellular proliferation. TORC1 primarily implicated linking amino acid levels yeast mammals. Although glucose deprivation has been shown to cause inactivation yeast, precise role signaling underlying mechanisms remain unclear. In this paper, we demonstrate medium both necessary sufficient for activation. activity increases...