Rebecca Chiu
A5104278613- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Tryptophan and brain disorders
- Computational Drug Discovery Methods
- Alzheimer's disease research and treatments
- Chemokine receptors and signaling
- Cancer therapeutics and mechanisms
- Bioinformatics and Genomic Networks
- Machine Learning in Bioinformatics
- Immune Response and Inflammation
- Synthesis and biological activity
- Neurogenesis and neuroplasticity mechanisms
Columbia University
2024-2025
Columbia University Irving Medical Center
2024-2025
Abstract While efforts to identify microglial subtypes have recently accelerated, the relation of transcriptomically defined states function has been largely limited in silico annotations. Here, we characterize a set pharmacological compounds that proposed polarize human microglia towards two distinct – one enriched for AD and MS genes another characterized by increased expression antigen presentation genes. Using different model systems including HMC3 cells, iPSC-derived cerebral organoids,...
Human microglial heterogeneity is only beginning to be appreciated at the molecular level. Here, we present a large, single-cell atlas of expression signatures from 441,088 live microglia broadly sampled across diverse set brain regions and neurodegenerative neuroinflammatory diseases obtained 161 donors autopsy or during neurosurgical procedure. Using hierarchical Poisson factorization (scHPF), derived 23-factor model for continuous gene which capture specific biological processes (e.g.,...
Abstract Genomic studies at single-cell resolution have implicated multiple cell types associated with clinical and pathological traits in Alzheimer’s Disease (AD), but not examined common features across broad, multi-ethnic populations, regions. To bridge this gap, we performed single-nucleus RNA-seq ATAC-seq profiling of cortical subcortical brain regions from post-mortem samples Non-Latin White, African American, Latin donors (the latter any race). Using discrete continuous dissection...
<title>Abstract</title> Human microglial heterogeneity has been largely described using transcriptomic data. Here, we introduce a proteomic data resource and Cellular Indexing of Transcriptomes Epitopes by Sequencing panel enhanced with antibodies targeting 17 cell surface proteins (mCITE-Seq). We evaluated mCITE-Seq on HMC3 microglia-like cells, induced-pluripotent stem cell-derived microglia (iMG), freshly isolated primary human microglia. identified novel protein markers such as CD51...
Summary Background Microglia, the brain’s resident immune cells, play vital roles in brain development, and disorders like Alzheimer’s disease (AD). Human iPSC-derived microglia (iMG) provide a promising model to study these processes. However, existing iMG generation protocols face challenges, such as prolonged differentiation time, lack of detailed characterization, limited gene function investigation via CRISPR-Cas9. Methods Our integrated toolkit for in-vitro functional genomics...
Human microglial heterogeneity has been largely described using transcriptomic data. Here, we introduce a proteomic data resource and Cellular Indexing of Transcriptomes Epitopes by Sequencing panel enhanced with antibodies targeting 17 cell surface proteins (mCITE-Seq). We evaluated mCITE-Seq on HMC3 microglia-like cells, induced-pluripotent stem cell-derived microglia (iMG), freshly isolated primary human microglia. identified novel protein markers such as CD51 relate expression 101 to...
While efforts to identify microglial subtypes have recently accelerated, the relation of transcriptomically defined states function has been largely limited in silico annotations. Here, we characterize a set pharmacological compounds that proposed polarize human microglia towards two distinct – one enriched for AD and MS genes another characterized by increased expression antigen presentation genes. Using different model systems including HMC3 cells, iPSC-derived cerebral organoids, effect...