Objective Currently, it has been reported that uterine microbiota affected pregnancy outcomes in assisted reproductive technology, but little was known obese women. Thus, this study aims to explore how body weight affects by comparing the differences between women of normal and those with obesity or overweight. Methods The included 45 embryo transfer cycles. Patients were divided into two groups based on mass index (BMI): normal-weight group, BMI ≤23.9 kg/m² (Con n=31) obesity/overweight...

Abstract Background Isothiocyanates are natural compounds found in consumable cruciferous vegetables. They have been shown to inhibit chemical carcinogenesis by a wide variety of carcinogens animal models. Recent studies also that isothiocyanates antitumor activity, inhibiting the growth several types cultured human cancer cells. Our previous study showed PEITC inhibited leukemia cells inducing apoptosis. However, effect on lung cell metastasis has not studied. In present study, we...

Human antioxidant‐response element (hARE) containing two copies of the AP1/AP1‐like elements arranged as inverse repeat is known to mediate basal and β‐naphthoflavone‐induced transcription type 1 NAD(P)H:quinone oxidoreductase (NQO ) gene. Band‐shift assays revealed that β‐naphthoflavone increased binding nuclear proteins at hARE. Super shift identified Jun‐D c‐Fos in band‐shift complexes observed with control β‐naphthoflavone‐treated Hepa‐1 extracts. cells stably transformed...

Bacterial magnetosomes (BMs) have drawn great interest as novel magnetic targeted carriers and been widely used for enzymes, nucleic acids antibodies experimentally. However, BMs rarely employed drug in vivo mainly due to the unclear biocompatibility pharmacokinetics of BMs. In this study, we provided a unique effective method purification sterilization The obtained exhibited sterility, high purity, narrowed size-distribution, uniformity morphology, intact membrane abundant amino groups...

Yiqi Huoxue Recipe (YHR) is commonly used in China to treat diseases such as heart failure (HF). It has been reported that YHR can HF and a certain protective effect on myocardial cell damage. The purpose of this study determine the cardioprotective effects HF-induced apoptosis clarify its mechanism action. Oxygen glucose deprivation/recovery (OGD/R) induces H9C2 model. Ligation left anterior descending artery (LAD) coronary induce an animal model HF. We found protected cells from...

Abstract Background Nuclear factor E2-related 2 (Nrf2) is an important transcription which plays a pivotal role in detoxifying reactive oxygen species (ROS) and has been more recently shown to regulate inflammatory antiviral responses. However, the of Nrf2 Herpes Simplex Virus type 1 (HSV-1) infection still unclear. In this study, interaction between HSV-1 replication was investigated. Methods The levels oxidative stress monitored by using 8-hydroxy-2'-deoxyguanosine (8-OHdG) ELISA kits,...

Oxidative stress contributes greatly to initiation and progression of liver injury. Activation nuclear-factor erythroid 2-related factor 2 (Nrf2) has been considered as an attractive strategy for preventing treating the oxidative damage related This study aimed find efficacious agent activate Nrf2/HO-1 signaling pathway from clinically used therapeutic agents characterize usefulness CCl4-induced acute For this purpose, a series were collected their activation potentials on Nrf2 assayed by...

Cinnamon aqueous extract's active substance base remains unclear and its mechanisms, mainly the therapeutic target of anti-Alzheimer's disease (AD)-related GABAergic synaptic dysfunction, remain unclear. Here, 30 chemical components were identified in extract cinnamon using LC/MS; secondly, we explored brain-targeting cinnamon, 17 had a good absorption due to blood-brain barrier (BBB) limitation; thirdly, further clustering analysis ingredient targets by network pharmacology showed that GABA...

Pterostilbene (PTE), a dietary derivative of resveratrol, displayed pleiotropic health-promoting activities. This study aimed to explore the metabolic profiles and species differences phase I metabolism PTE investigate subsequent detoxification after bioactivation. was found be biotransformed two pharmacologically active metabolites, pinostilbene 3'-hydroxypterostilbene, in vivo vitro with substantial differences. Human CYP1A2 proved mainly responsible for demethylation 3'-hydroxylation PTE,...

Traumatic neuroma is a common sequela of peripheral nerve injury or amputation, which often leads to severe neuropathic pain. The present study investigated the effect local lidocaine administration on preventing formation traumatic neuroma. Forty-eight male Sprague-Dawley rats were randomly assigned two groups. group underwent sciatic transection, followed by an injection (0.5%) around proximal severed under ultrasound-guidance 2-7 days after neurectomy. In control group, received saline...

Gynura segetum (Lour.) Merr. (GS), has been widely used in Chinese folk medicine and can promote circulation, relieve pain remove stasis. In recent years, the hepatotoxicity caused by GS reported, however its mechanism is not fully elucidated. Metabolomic techniques are powerful means to explore toxicological therapeutic effects of traditional medicine. The purpose this study was establish a serum metabolomics method based on Gas Chromatography-Mass Spectrometry (GC-MS) different exposure...

This study aimed to assess the efficacy and safety of remifentanil as a general anesthetic during cesarean delivery.Fifty women with singleton pregnancies undergoing delivery were randomly divided into intervention control groups, each group containing 25 subjects. Participants in received (infused at 2 μg/kg/h), whereas subjects given dexmedetomidine 0.4 μg/kg/h). Outcome measurements included mean arterial blood pressure (MAP), heart rate (HR), bispectral index (BIS), Apgar scores 1 5...

Little is known about the effect of electroacupuncture (EA) on cerebral blood flow. We investigated this question in patients undergoing laparoscopic cholecystectomy, hypothesizing that EA would increase flow during surgery.

Background: The purpose of the research is to identify main active ingredients in Coptidis Rhizoma (CR) and explore possible molecular mechanisms treatment Kawasaki disease (KD). Materials Methods: A total 58 children with KD were randomly divided into a control group Berberine group. therapeutic indicators two groups before after compared. Then, compounds drug targets CR from TCMSP, SWISS, SEA, STITCH collected, targeted genes retrieved DisGeNET, DrugBank, GeneCards databases. network...

Objective To discuss a design of flap dorsal branches the digital proper artery to repair pediatric middle phalanx skin defects and its effect. Methods From October, 2013 March, 2015, antegrade flaps were used treat 9 patients with defect in phalanx, as pedicel. The branch nerve carried by was sutured wound surface cutting area 3.5 cm × 1.2 - 2.5 0.8 cm, donor site repaired full thickness graft. Results All survived, primary healing recipient site. followed up for 6-12 months,...

Abstract This study aims to investigate the relevant mechanism by which hydrazinocurcumin (HC) interferes with A431 cell autophagy inhibiting STAT3 signaling pathway. Different concentrations of HC are used treat cells effects on proliferation and apoptosis. Real‐time fluorescent quantitative polymerase chain reaction (PCR) is further explore relationship JAK pathway autophagy. Double immunofluorescence staining detect fluorescence localization LC3 after treatment. With increasing...

Abstract Lung cancer is one of the most aggressive types, and leading cause cancer-related deaths worldwide. Metastasis great importance to clinical management since it causes 90% from solid tumours. Therefore development novel anti-metastasis drugs becomes active fields in research. Isothiocyanates are natural compounds found consumable cruciferous vegetables. They have been shown ability inhibit chemical carcinogenesis by a wide variety carcinogens animal models, inhibiting tumorigenesis...

Objective To compare the efficacy of two regimens volume therapy in patients undergoing radical intestinal cancer operation. Methods Forty ASA Ⅰ or Ⅱ patients, aged 45-64 yr, weighing 42-65 kg,scheduled for operation, were randomly divided into group and (n = 20 each).In , 6% hydroxyethyl starch 130/0.4 (HES) 15 ml/kg was infused iv over 30 min before anesthesia induction, lactated Ringer's solution immediately after anesthesiainduction hemodilution. In HES induction Intraoperative infusion...