Abstract The rapid high-throughput detection of foodborne pathogens is essential in controlling food safety. In this study, a 10-channel up-converting phosphor technology-based lateral flow (TC-UPT-LF) assay was established for the and simultaneous 10 epidemic pathogens. Ten different single-target UPT-LF strips were developed integrated into one TC-UPT-LF disc with optimization. Without enrichment had sensitivity 4 CFU mL −1 or 5 each pathogen, after sample it CFU/0.6 mg. also showed good...

Abstract Modern microscopes used for biological imaging often present themselves as black boxes whose precise operating principle remains unknown, and optical resolution price seem to be in inverse proportion each other. With UC2 (You. See. Too.) we a low-cost, 3D-printed, open-source, modular microscopy toolbox demonstrate its versatility by realizing complete microscope development cycle from concept experimental phase. The self-contained incubator-enclosed brightfield monitors monocyte...

Flexible and multifunctional electronic skin (e-skin) has received remarkable attention for its potential applications in health monitoring, human-machine interface, artificial sensory nerves. However, conventional e-skins require complex material systems, sophisticated fabrication, external power supplies, leading to increased preparation cost duration, thus hindering their large-scale utilization. Herein, a self-powered e-skin system with properties of pressure, temperature, underwater...

Abstract 3D printing of microfluidic lab-on-a-chip devices enables rapid prototyping robust and complex structures. In this work, we designed fabricated a printed device for fiber-based dual beam optical manipulation. The final chip offers three key features, such as (1) an optimized fiber channel design precise alignment fibers, (2) optically clear window to visualize the trapping region, (3) sample which facilitates hydrodynamic focusing samples. A square zig–zag structure incorporated in...

Abstract The number of samples in biological experiments is continuously increasing, but complex protocols and human error many cases lead to suboptimal data quality hence difficulties reproducing scientific findings. Laboratory automation can alleviate these problems by precisely machine‐readable protocols. These instruments generally require high up‐front investments, due the lack open application programming interfaces (APIs), they are notoriously difficult for scientists customize...

Abstract In biological imaging, there is a demand for cost-effective, high-resolution techniques to study dynamic intracellular processes. Structured illumination microscopy (SIM) ideal achieving high axial and lateral resolution in live samples due its optical sectioning low phototoxicity. However, conventional SIM systems remain expensive complex. We introduce openSIMMO, an open-source, fully-automated module compatible with commercial microscopes, supporting dual-color excitation. Our...

We present a novel open-source modular electro-optical toolbox to be used for many different optical setups. Based on off-the-shelf components we show live-cell imaging device and an super-resolution microscope using Image-Scanning-Microscopy (ISM) techniques.

State-of-the-art microscopy techniques enable the imaging of sub-diffraction barrier biological structures at price high costs or a lack transparency. We try to reduce some these barriers by presenting super-resolution upgrade our recently presented open-source optical toolbox UC2. Our new injection moulded parts allow larger builds with higher precision. The 4× lower manufacturing tolerance compared three-dimensional printing makes assemblies more reproducible. By adding consumer-grade...

Paper and textile are two ideal carriers in wearable printed electronics because of their flexibility low price. However, the porous fibrous structures restrain use capillary effect results ink diffusion. Especially, conventional metal needs to be post-treated at high temperatures (>150 °C), which is not compatible with paper textile. To address problems involved diffusion avoid high-temperature treatment, herein, a new strategy proposed: screen-printing high-viscosity catalytic inks...

Liquid-metal (LM) electrical switches have been desired for decades to overcome intrinsic shortcomings of traditional mechanical equipped with solid contacts. Existing LM switches, functioning via solid-liquid wetting or liquid droplet actuation, mostly focus on low current and small signal switches. In this Letter, a switch aiming at power-level has proposed. A proof-of-concept prototype was fabricated encapsulating Galinstan droplets into silicone matrix. The device circuit when are...

Abstract Since the development of first light microscope over 400 years ago, technology has continuously evolved and established itself as a powerful tool, especially in biology, diagnostics point-of-care (PoC) applications. The miniaturization mass-produced actuators sensors enables use technically extremely complex functions smartphones at very low price. They can be used to implement modern microscopy methods for places where access such techniques is often limited. In this review, we...

Abstract State-of-the-art microscopy techniques enable the imaging of sub-diffraction barrier biological structures at price high-costs or lacking transparency. We try to reduce some these barriers by presenting a super-resolution upgrade our recently presented open-source optical toolbox UC2. Our new injection moulded parts allow larger builds with higher precision. The 4× lower manufacturing tolerance compared 3D printing makes assemblies more reproducible. By adding consumer-grade...

The number of samples in biological experiments are continuously increasing, but complex protocols and human experimentation many cases lead to suboptimal data quality hence difficulties reproducing scientific findings. Laboratory automation can alleviate these problems by precisely machine-readable protocols. These instruments generally require high up-front investments due lack open APIs they notoriously difficult for scientists customize control outside the vendor-supplied software. Here,...

Abstract With UC2 (You-See-Too) we present an inexpensive 3D-printed microscopy toolbox. The system is based on concepts of modular development, rapid-prototyping and all-time accessibility using widely available off-the-shelf optic electronic components. We aim to democratize microscopy, reduce the reproduction crisis enhance trust into science by making it everyone via open-access public repository. Due its versatility boost creativity non-conventional approaches. In this paper,...

Chemically pretreating lignocellulose has been widely used in the production of clean energy. An energy grass called hybrid Pennisetum was pretreated with different H2SO4 and NaOH concentrations to analyse effects an acid alkali pretreatment on degradation lignocellulose. The chemical composition, hydrolyzed sugar yield, microstructure were determined. results showed that a retained more cellulose removed lignins, while significantly inhibited removal lignins increased hemicellulose...

In order to impart nano hydroxyapatite (HA) more biological functions and develop its potential application in biomedical field, silver modified (Ag-HA) nanoparticles were synthesized via sol–gel method. The powders sintered at 600 °C for 1 h. microstructures, morphologies chemical compositions of HA Ag-HA investigated by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), photoelectron (XPS), High resolution transmission electron microscopy (HR-TEM), Scanning (SEM)...

Abstract In the rapidly advancing field of biological imaging, there is a great need for high-resolution imaging techniques that are both cost-effective and accessible, example to better observe understand dynamics in intracellular processes. Structured illumination microscopy (SIM) method choice achieve high axial lateral resolution living samples due its optical sectioning minimal phototoxicity. However, cost complexity conventional SIM systems limit their wide application. our work, we...

Herein, we developed a sensitive, selective, efficient, and low-cost method for higenamine (HG) detection. This employs high-performance liquid chromatography with chemiluminescence detector (HPLC-CL) coupled an AgIII-luminol system. Lotus plumule human urine samples were purified using mixed weak cation exchange column (PWCX) solid-phase extraction cartridge, C18 was employed chromatographic separation aqueous solution of acetonitrile 0.1% formic acid as the mobile phase. Under optimal...