A5016984840- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Cell Image Analysis Techniques
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
Radboud University Nijmegen
2021-2024
Radboud University Medical Center
2021-2024
Radboud Institute for Molecular Life Sciences
2021
Vrije Universiteit Amsterdam
2019
Amsterdam University Medical Centers
2019
Abstract Metastatic colorectal cancer (CRC) is highly resistant to therapy and prone recur. The tumor‐induced local systemic immunosuppression allows cells evade immunosurveillance, facilitating their proliferation dissemination. Dendritic (DCs) are required for the detection, processing, presentation of tumor antigens, subsequently activation antigen‐specific T orchestrate an effective antitumor response. Notably, successful tumors have evolved mechanisms disrupt impair DC functions,...
Squamous cell carcinoma (SCC) and adenocarcinoma (AC) represent the major cervical cancer histotypes. Both histotypes are caused by infection with high-risk HPV (hrHPV) associated deregulated microRNA expression. Histotype-dependent expression has been observed for miR-9-5p, showing increased in SCC low AC. Here, we studied regulation functionality of miR-9-5p SCCs ACs using tissue samples hrHPV-containing lines. Expression methylation analysis tissues revealed that levels linked to its...
Abstract Dendritic cells (DCs) are essential in antitumor immunity. In humans, three main DC subsets defined: two types of conventional DCs (cDC1s and cDC2s) plasmacytoid (pDCs). To study the tumor microenvironment (TME), it is important to correctly identify them tissues. Tumor‐derived often analyzed cell suspensions which spatial information about can be determine their function within TME lost. Therefore, we developed first standardized optimized multiplex immunohistochemistry panel,...
The immune cell landscape of the tumor microenvironment potentially contains information for discovery prognostic and predictive biomarkers. Multiplex immunohistochemistry is a valuable tool to visualize identify different types cells in tissues while retaining its spatial information. Here we provide detailed protocols analyze lymphocyte, myeloid, dendritic populations tissue sections. Starting from cutting formalin-fixed paraffin-embedded sections, automatic multiplex staining procedures...