A5012389291- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Neonatal Respiratory Health Research
- Single-cell and spatial transcriptomics
- Congenital Diaphragmatic Hernia Studies
- Renal and related cancers
- Genetics, Aging, and Longevity in Model Organisms
- Histone Deacetylase Inhibitors Research
- Respiratory viral infections research
- Protein Degradation and Inhibitors
- Immune cells in cancer
- Respiratory Support and Mechanisms
- Energy Harvesting in Wireless Networks
- Hepatitis C virus research
- Ethics and Legal Issues in Pediatric Healthcare
- Diabetes and associated disorders
- Epigenetics and DNA Methylation
- Mesenchymal stem cell research
- Liver Disease Diagnosis and Treatment
- Chemokine receptors and signaling
Korea National Institute of Health
2022-2025
Bank of Korea
2022-2024
Within the human lung, interactions between alveolar epithelial cells and resident macrophages shape lung development function in both health disease. To study these processes, we develop a co-culture system combining pluripotent stem cell-derived organoids induced to create functional environment, termed assembloids. Using single-cell RNA sequencing analyses, identify type 2-like producing GM-CSF, which supports macrophage tissue adaptation, macrophage-like that secrete interleukin-1β...
HCV infection can be successfully managed with antiviral therapies; however, progression to chronic liver disease states, including NAFLD, is common. There currently no reliable in vitro model for investigating host-viral interactions underlying the link between and NAFLD; although organoids (LOs) show promise, they lack nonparenchymal cells, which are key modeling progression.
Patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A virus (FLUAV) coinfections were associated failure more deaths. Here, we developed a model for studying SARS-CoV-2 FLUAV coinfection using human pluripotent stem cell-induced alveolar type II organoids (hiAT2). hiAT2 susceptible to infection by both viruses had features of lung damage. single markedly enhanced the susceptibility other infections. delta variants upregulated α-2-3-linked sialic acid,...
During in vitro culture, human pluripotent stem cells (hPSCs) often acquire survival advantages characterized by decreased susceptibility to mitochondrial cell death, known as "culture adaptation." This adaptation is associated with genetic and epigenetic abnormalities, including TP53 mutations, copy number variations, trisomy, methylation changes. Understanding the molecular mechanisms underlying this acquired advantage crucial for safe hPSC-based therapies. Through transcriptome methylome...
Macrophages exhibit high plasticity to achieve their roles in maintaining tissue homeostasis, innate immunity, repair and regeneration. Therefore, macrophages are being evaluated for cell-based therapeutics against inflammatory disorders cancer. To overcome the limitation related expansion of primary cell numbers, human pluripotent stem (hPSC)-derived considered as an alternative source clinical application. However, quality hPSC-derived with respect biological homogeneity remains still...
(1) Background: An important concomitant of stroke is neuroinflammation. Pomalidomide, a clinically available immunomodulatory imide drug (IMiD) used in cancer therapy, lowers TNF-α generation and thus has potent anti-inflammatory actions. Well-tolerated analogs may provide treatment allow evaluation the role neuroinflammation ischemic brain. (2) Methods: Two novel pomalidomide derivatives, 3,6′-dithiopomalidomide (3,6′-DP) 1,6′-dithiopomalidomide (1,6′-DP), were evaluated alongside rat...
Cultured human pluripotent stem cells (hPSCs) grow as colonies that require breakdown into small clumps for further propagation. Although cell death mechanism by single-cell dissociation of hPSCs has been well defined, how respond to the deadly stimulus and recover original status remains unclear. Here we show immediately activates ERK, which subsequently RSK induces DUSP6, an ERK-specific phosphatase. activation is transient, DUSP6 expression persists days after passaging. depletion using...
Abstract Human embryonic stem cells (hESCs) are naturally equipped to maintain genome integrity minimize genetic mutations during early embryo development. However, aberration risks and subsequent cellular changes in hESCs vitro culture pose a significant threat cell therapy. While few studies have reported specific somatic copy number variations (CNVs), the molecular mechanisms underlying ‘culture-adapted phenotype’ acquisitions of largely unknown. Therefore, we conducted comprehensive...
Abstract Human embryonic stem cells (hESCs) are naturally equipped to maintain genome integrity minimize genetic mutations during early embryo development. However, aberration risks and subsequent cellular changes in hESCs vitro culture pose a significant threat cell therapy. While few studies have reported specific somatic copy number variations (CNVs), the molecular mechanisms underlying acquisition of ‘culture-adapted phenotypes’ by largely unknown. Therefore, we conducted comprehensive...