A5038366276- Amyotrophic Lateral Sclerosis Research
- Muscle Physiology and Disorders
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Cell Image Analysis Techniques
- Mitochondrial Function and Pathology
- Advanced Fluorescence Microscopy Techniques
- Ubiquitin and proteasome pathways
- Genetics, Aging, and Longevity in Model Organisms
- Silk-based biomaterials and applications
- Autophagy in Disease and Therapy
Weizmann Institute of Science
2018-2024
Myoblast fusion is essential for muscle development and regeneration. Yet, it remains poorly understood how mononucleated myoblasts fuse with preexisting fibers. We demonstrate that ERK1/2 inhibition (ERKi) induces robust differentiation of primary mouse through a linear pathway involving RXR, ryanodine receptors, calcium-dependent activation CaMKII in nascent myotubes. results myotube growth via at fusogenic synapse. Mechanistically, interacts regulates MYMK Rac1, CaMKIIδ/γ knockout mice...
Abstract The role of mitochondria dynamics and its molecular regulators remains largely unknown during naïve-to-primed pluripotent cell interconversion. Here we report that mitochondrial MTCH2 is a regulator fusion, essential for the interconversion murine embryonic stem cells (ESCs). During this interconversion, wild-type ESCs elongate their slightly alter glutamine utilization. In contrast, −/− fail to metabolism, maintaining high levels histone acetylation expression naïve pluripotency...
Abstract Systematic assessment of organelle architectures in cells, known as the organellome, could provide valuable insights into cellular states and disease pathologies but remains largely uncharted. Here, we devised a novel pipeline combining self-supervised deep learning transfer to generate Neuronal Organellomics Vision Atlas (NOVA). Analyzing over 1.5 million confocal images 24 distinct membrane-bound membrane-less organelles human neurons, enable simultaneous evaluation all...