A5018222475- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Cancer Genomics and Diagnostics
- Cytomegalovirus and herpesvirus research
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Molecular Biology Techniques and Applications
- Evolution and Genetic Dynamics
Gladstone Institutes
2022-2025
Stanford University
2022-2025
Harvard University
2025
Memorial Sloan Kettering Cancer Center
2024
Summary Paragraph Somatic variation contributes to biological heterogeneity by modulating cellular proclivity differentiate, expand, adapt, or die. While large-scale sequencing efforts have revealed the foundational role of somatic variants drive human tumor evolution, our understanding contribution mutations modulate fitness in non-malignant contexts remains understudied. Here, we identify a mosaic synonymous variant (m.7076A>G) mitochondrial DNA (mtDNA) encoded cytochrome c-oxidase...
This protocol can be used for performing HCR Flow-FISH followed by cell/nuclei enrichment via FACS and polymer disassembly on fixed permeabilized cells tissue derived nuclei. should performed after finalizing "PERFF-seq: Cell Nuclei Preparation" the desired sample type.
This protocol can be used for preparation of Cells and Fresh-Frozen Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Derived Nuclei. should followed by "PERFF-seq: HCR-FlowFISH Polymer Disassembly."
Abstract Single-cell genomics technologies have accelerated our understanding of cell-state heterogeneity in diverse contexts. Although single-cell RNA sequencing (scRNA-seq) identifies many rare populations interest that express specific marker transcript combinations, traditional flow sorting limits ability to enrich these for further profiling, including requiring cell surface markers with high-fidelity antibodies. Additionally, studies require the isolation nuclei from tissue,...
Abstract Cell therapies have yielded durable clinical benefits for patients with cancer, but the risks associated development of from manipulated human cells are still being understood. For example, we currently lack a comprehensive understanding mechanisms neurotoxicity observed in receiving T cell therapies, including recent reports encephalitis caused by herpesvirus 6 (HHV-6) reactivation 1 . Here, via petabase-scale viral RNA data mining, examine landscape latent and demonstrate that...