A5101559804- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- DNA and Nucleic Acid Chemistry
- Molecular Biology Techniques and Applications
- Electrochemical Analysis and Applications
- Glycosylation and Glycoproteins Research
- Human Mobility and Location-Based Analysis
- X-ray Diffraction in Crystallography
- Inflammasome and immune disorders
- Consumer Retail Behavior Studies
- RNA Interference and Gene Delivery
- Advanced Biosensing Techniques and Applications
- Polysaccharides and Plant Cell Walls
- RNA and protein synthesis mechanisms
- Nanopore and Nanochannel Transport Studies
- Antibiotics Pharmacokinetics and Efficacy
- Protein Interaction Studies and Fluorescence Analysis
- Crystallization and Solubility Studies
- Customer Service Quality and Loyalty
- Carbohydrate Chemistry and Synthesis
- Gene expression and cancer classification
- Membrane-based Ion Separation Techniques
- Mathematical and Theoretical Epidemiology and Ecology Models
- Drug Transport and Resistance Mechanisms
- Hemodynamic Monitoring and Therapy
Sichuan University
2023-2024
State Key Laboratory of Oral Diseases
2023-2024
Harvard University
2017-2024
Center for Systems Biology
2017-2024
Seoul National University
2023
National Clinical Research
2023
University of Science and Technology of China
2020-2021
State Key Laboratory of Polymer Physics and Chemistry
2020-2021
Chinese Academy of Sciences
2020-2021
Changchun Institute of Applied Chemistry
2020-2021
We present a nonlinear hybridization chain reaction (HCR) system in which trigger DNA initiates self-sustained assembly of quenched double-stranded substrates into fluorescent dendritic nanostructures. During the process, an increasing number originally sequestered sequences labeled with reporters are freed up from substrates, leading to chain-branching growth assembled dendrimers and exponential increase fluorescence intensity. The triggered behavior was examined by PAGE analysis,...
Hg(2+) is known to bind very strongly with T-T mismatches in DNA duplexes form T-Hg(2+)-T base pairs, the structure of which stabilized by covalent N-Hg bonds and exhibits bonding strength higher than hydrogen bonds. In this work, we exploit exonuclease III (Exo III) activity on hybrids containing pairs our experiments show that ions could intentionally trigger Exo toward a designed thymine-rich oligonucleotide (e-T-rich probe) conformational change probe. Our sensing strategy utilizes...
Taking advantage of the preferential exodeoxyribonuclease activity exonuclease III in combination with difference diffusivity between an oligonucleotide and a mononucleotide toward negatively charged ITO electrode, highly sensitive selective electrochemical molecular beacon (eMB)-based DNA sensor has been developed. This realizes detection homogeneous solution, sensing signals amplified by III-based target recycling strategy. A hairpin-shaped containing recognition sequence, methylene blue...
We present an immobilization-free and enzyme-free electrochemical nucleic acid sensing strategy, which uses kinetically controlled dendritic assembly of DNA peptide (PNA). In the presence a target sequence, ferrocene-labeled PNA probes (Fc-PNAs) specially designed strands are autonomously assembled into nanostructures through cascade toehold-mediated strand displacement reactions. The consumption freely diffusible Fc-PNAs (neutrally charged), due to incorporation DNA/PNA dendrimer, results...
Synaptic vesicles (SVs) can be pooled across multiple synapses, prompting questions about their dynamic allocation for neurotransmission and plasticity. We find that the axonal traffic of recycling is not supported by ubiquitous microtubule-based motility but relies on actin instead. Vesicles freed from synaptic clusters undergo ~1 µm bouts active transport, initiated nearby elongation filaments. Long distance translocation arises when successive transport were linked periods free diffusion....
Analysis of the spatial arrangement molecular features enables engineering synthetic nanostructures and understanding natural ones. The ability to acquire a comprehensive set pairwise proximities between components would satisfy an increasing interest in investigating individual macromolecules their interactions, but current biochemical techniques detect only single proximity partner per probe. Here, we present DNA nanoscopy method that records nanostructure situ detail for later readout....
Abstract DNA-based artificial motors have allowed the recapitulation of biological functions and creation new features. Here, we present a molecular robotic system that surveys environments reports spatial information in an autonomous repeated manner. A group agents, termed ‘crawlers’, roam around copy from DNA-labeled targets, generating records reflect their trajectories. Based on mechanism allows random crawling, show our is capable counting number subunits example complexes. Our can also...
The molecular recognition and binding interaction of beta-lactamase II from Bacillus cereus (Bc II) with penicillin V (PV) sulbactam (Sul) at 277 K were studied by spectroscopic analysis docking. results showed that a non-fluorescence static complex was separately formed between Bc two ligands, the ratio to PV or Sul both 1:1 in constants 2.00 × 106 3.98 105 (L/mol), respectively. negative free energy changes apparent activation energies indicated processes spontaneous. Molecular docking...
Abstract Background Cutting‐edge ultrasensitive immunoassay platforms have unveiled the potential of blood‐based biomarkers, offering detection at low fg/mL levels for early neurodegenerative disorder prognosis, screening, and therapeutic monitoring. Current immunoassays, such as single molecule array (SIMOA) mesoscale multi‐array (MSD), face limited adoption due to their reliance on specialized equipment. Additionally, they require immobilization probe reagents a washing process, demanding...
Periodontitis, arguably the greatest common infective chronic inflammatory disease, is characterized by an imbalance of host immune system and excessive osteoclastogenesis activity with severe alveolar bone loss. Nevertheless, in consideration harmful effects repeated treatment, more sensible intervention drugs for periodontitis need to be developed. Artesunate (ART), derived from Artemisia annua L., has shown remarkable pharmacokinetic clinical value, as well anti-inflammatory...
Post-combustion capture has been an important field for CO2 capture, but its high H2O content a significant barrier to the performance of MMMs. In this study, series humidity was established, and two types MOFs (SUM-1 SUM-9) were used in Pebax 2533 prepare MMMs explore CO2/N2 separation performance. At 35 ℃ 2 bar, permeability Pebax-SUM-1 380.87 Barrer, 433.06 405.43 401.72 respectively, when relative changed from 0%, 30%, 60%, 100%. The Pebax-SUM-9 390.87 387.41 412.50 452.44 respectively....
With the development of information age, cities provide a large amount data that can be analyzed and utilized to facilitate decision-making process. Urban big analytics are particularly valuable in analysis business location decisions, providing insight supporting informed choices. By examining relating commercial locations, it becomes possible analyze various spatial characteristics derive feasibility different locations. This analytical approach contributes effective formulation robust...
With the development of information age, cities provide a large amount data that can be analyzed and utilized to facilitate decision-making process. Urban big analytics are particularly valuable in analysis business location decisions, providing insight supporting informed choices. By examining relating commercial locations, it becomes possible analyze various spatial characteristics derive feasibility different locations. This analytical approach contributes effective formulation robust...