A5087941971- Inflammasome and immune disorders
- Immune Response and Inflammation
- Heme Oxygenase-1 and Carbon Monoxide
- Streptococcal Infections and Treatments
- Yersinia bacterium, plague, ectoparasites research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Escherichia coli research studies
- Vibrio bacteria research studies
- Toxin Mechanisms and Immunotoxins
- Marine Invertebrate Physiology and Ecology
- Gout, Hyperuricemia, Uric Acid
Institut de Pharmacologie et de Biologie Structurale
2022-2025
Centre National de la Recherche Scientifique
2023-2025
Université de Toulouse
2023
Nigericin, an ionophore derived from Streptomyces hygroscopicus , is arguably the most commonly used tool compound to study NLRP3 inflammasome. Recent findings, however, showed that nigericin also activates NLRP1 inflammasome in human keratinocytes. In this study, we resolve mechanistic basis of nigericin-driven activation. multiple nonhematopoietic cell types, rapidly and specifically inhibits elongation stage ribosome cycle by depleting cytosolic potassium ions. This ribotoxic stress...
Multiple regulated neutrophil cell death programs contribute to host defense against infections. However, despite expressing all necessary inflammasome components, neutrophils are thought be generally defective in Caspase-1-dependent pyroptosis. By screening different bacterial species, we found that several Pseudomonas aeruginosa ( P . ) strains trigger pyroptosis human and murine neutrophils. Notably, deletion of Exotoxins U or S enhanced pyroptosis, suggesting these exotoxins interfere...
Human airway and corneal epithelial cells, which are critically altered during chronic infections mediated by Pseudomonas aeruginosa, specifically express the inflammasome sensor NLRP1. Here, together with a companion study, we report that NLRP1 detects exotoxin A (EXOA), ribotoxin released P. aeruginosa type 2 secretion system (T2SS), infection. Mechanistically, EXOA-driven eukaryotic elongation factor (EEF2) ribosylation covalent inactivation promote ribotoxic stress subsequent activation,...
Abstract In 2020–2021, a “mysterious illness” struck Senegalese fishermen, causing severe acute dermatitis in over one thousand individuals following exposure through drift-net fishing activity. Here, by performing deep analysis of the environmental samples we reveal presence marine dinoflagellate Vulcanodinium rugosum and its associated cyclic imine toxins. Specifically, show that toxin PortimineA, strongly enriched samples, impedes ribosome function human keratinocytes, which subsequently...
ABSTRACT The intracellular inflammasome complex have been implicated in the maladaptive tissue damage and inflammation observed chronic Pseudomonas aeruginosa infection. Human airway corneal epithelial cells, which are critically altered during infections mediated by P. , specifically express sensor NLRP1. Here, together with a companion study, we report that NLRP1 detects Exotoxin A (EXOA), ribotoxin released Type 2 Secretion System (T2SS) Mechanistically, EXOA-driven Eukaryotic Elongation...