A5102762036- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Immune cells in cancer
- Advanced Fluorescence Microscopy Techniques
- Microscopic Colitis
- Inflammatory Bowel Disease
- Cancer Genomics and Diagnostics
- Neuroblastoma Research and Treatments
- Cell Image Analysis Techniques
- Genetic factors in colorectal cancer
- Cancer Immunotherapy and Biomarkers
University Medical Center Utrecht
2021-2024
Utrecht University
2021-2023
Exploring tissue heterogeneity on a single-cell level by imaging mass cytometry (IMC) remains challenging because of its limiting resolution. We previously demonstrated that combining higher resolution fluorescence with IMC data in the analysis pipeline resulted high-quality segmentation. Here, we provide step-by-step workflow this MATISSE pipeline, including instructions regarding staining procedure, and route to generate data. For complete details use execution protocol, please refer Baars...
<h3>Background</h3> The development of immunotherapeutic agents has transformed the treatment neuroblastoma patients. Nevertheless, efficacy these immunotherapies is challenged by highly immunosuppressive microenvironment, which hampers immune cell migration into tumor area. Here, we aim to identify targeted strategies enhance infiltration, mapping microenvironment (TIME) and cell-cell interactions in using imaging mass cytometry (IMC). <h3>Materials Methods</h3> We used IMC profile <i>in...
Summary Colitis is a prevalent adverse event associated with immune checkpoint inhibitor (ICI) therapy similarities to inflammatory bowel disease. Incomplete mechanistic understanding of ICI-colitis curtails evidence-based treatment. Given the often-overlooked connection between tissue architecture and mucosal cell function, we here applied imaging mass cytometry (IMC) gain spatial proteomic insight in comparison ulcerative colitis (UC). Using segmentation pipeline that simultaneously...