A5046730013- Spectroscopy Techniques in Biomedical and Chemical Research
- Connexins and lens biology
- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Photodynamic Therapy Research Studies
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Nanoplatforms for cancer theranostics
- Spectroscopy and Chemometric Analyses
- Thermography and Photoacoustic Techniques
- Molecular Biology Techniques and Applications
- Congenital heart defects research
- Neuroscience and Neuropharmacology Research
- Hearing, Cochlea, Tinnitus, Genetics
- Tissue Engineering and Regenerative Medicine
- Neuroscience and Neural Engineering
- Colorectal Cancer Screening and Detection
- Radiomics and Machine Learning in Medical Imaging
- Photoreceptor and optogenetics research
- Cardiomyopathy and Myosin Studies
- Corneal Surgery and Treatments
- AI in cancer detection
- Heat shock proteins research
- Cardiovascular Effects of Exercise
- Corneal surgery and disorders
Kyoto Prefectural University of Medicine
2016-2025
Kyoto Prefectural University
2011
Kyoto University
1984-2011
Meiji University of Integrative Medicine
2011
Fukuyama City Hospital
2011
University of South Carolina
2005
Osaka University
2000
Louis Pasteur Center for Medical Research
1999
University of Maryland, Baltimore
1990
Shiga University
1984
A dual, digital, indo-1 fluorescence imaging system was used to obtain high-speed ratiometric images of [Ca2+]i waves in single voltage-clamped mammalian cardiac cells. The spatiotemporal origin depolarized cells detected as the spontaneous appearance, over 100-300 ms, domelike regions elevated [Ca2+]i, approximately 20 μm diameter and 300 nM at center. Images taken 67-ms intervals during propagation revealed that wave front 1) constant shape, 2) spatially steep, typically rising from 500...
Abstract We demonstrate long-range enhancement of fluorescence and Raman scattering using a dense random array Ag nanoislands (AgNIs) coated with column-structured silica (CSS) overlayer over 100 nm thickness, namely, remote plasmonic-like (RPE). The CSS layer provides physical chemical protection, reducing the impact between analyte molecules metal nanostructures. RPE plates are fabricated high productivity sputtering immersion in gold(I)/halide solution. plate significantly enhances...
Abstract —At the border zone of myocardial infarcts, surviving cardiomyocytes achieve drastic remodeling cell-cell and cell–extracellular matrix interactions. Spatiotemporal changes in these interactions are likely related to each other possibly have significant impact on cardiac function. To elucidate changes, we conducted experimental infarction rats performed 3-dimensional analysis localization gap junctions (connexin43), desmosomes (desmoplakin), adherens (cadherin), integrins (β 1...
Intracellular Ca 2+ waves (CaWs) of cardiomyocytes are spontaneous events release from the sarcoplasmic reticulum that regarded as an important substrate for triggered arrhythmias and delayed afterdepolarizations. However, little is known regarding whether or how CaWs within heart actually produce arrhythmogenic membrane oscillation because lack data confirming direct correlation between potentials ( V m ) in heart. On hypothesis evoke oscillatory depolarization when they emerge...
purpose. To explore connexin43 (Cx43) knockdown as an efficient treatment for corneal endothelial injury in vivo rat scrape model. methods. Scrape was induced the endothelium, and immunolabeling (ZO-1, α-SMA, Cx43) performed to analyze changes Cx43 expression during wound healing. Single injection of antisense oligodeoxynucleotide (AS-ODN), small interfering RNA (siRNA), or adenovirus (CMV-Cx43-mRFP1) applied into anterior chamber simultaneously with injury, closure examined by propidium...
The primary cilium is an antenna-like structure extending from the surface of most vertebrate cells. Loss or mutation ciliary proteins can lead to polycystic kidney disease and other developmental abnormalities. inv mutant mice develop multiple renal cysts are a model for human nephronophthisis type 2. mouse Inv gene encodes 1062-amino-acid protein that localized in cilia. In this study, we show (also known as inversin) at distinctive proximal segment cilium, using GFP-tagged constructs...
Transforming growth factor-beta (TGF-beta) superfamily members play an important role in growth, differentiation, adhesion, apoptosis, and development many species from insects worms to vertebrates. Recently, TGF-beta signaling has been demonstrated be negatively regulated by microtubules (MTs), which anchor endogenous Smad2/3 cytosol also directly interact with connexin43 (Cx43), the activity of is mediated Cx43. However, mechanism underlying intracellular regulation Cx43 remains unknown....
Background— Diabetic cardiomyopathy is characterized by energetic dysregulation caused glucotoxicity, lipotoxicity, and mitochondrial alterations. p53 its downstream assembly protein, synthesis of cytochrome c oxidase 2 (SCO2), are important regulators respiration, whereas the involvement in diabetic remains to be determined. Methods Results— The role SCO2 energy metabolism was examined both type I (streptozotocin [STZ] administration) II ( db/db ) mice. Cardiac expressions 4-week STZ mice...
Raman scattering of a cell conveys the intrinsic information inherent to chemical structures biomolecules. The spectroscopy scattering, or spectroscopy, allows label-free and quantitative molecular sensing biological sample in situ without disruption. For last five decades has been widely utilized research fields. However, it is just within latest decade that imaging discrimination living cells tissues have become practically available. Here we overview recent progress its application life...
Ploidy patterns of gastric carcinomas 54 Japanese patients, ranging in age from 28 to 82 years, were determined by cytofluorometry. The cancers divided into four basic ploidy patterns; the first pattern was a diploid mode, second heteroploid third mosaic and fourth two or more different modes. incidence 68.4%, 14.8%, 12.9%, 3.7% first, second, third, patterns, respectively. All contained polyploid populations, whereas early tended contain lesser amount cells than advanced ones. Diffuse-type...
Direct conversion of mammalian fibroblasts into induced neuronal (iN) cells has been attained by forced expression pro-neural transcriptional factors, or combining defined factors with either microRNAs small molecules. Here, we show that can be converted from postnatal human cell populations purities up to >80% using a combination six chemical compounds. The compound-induced (CiNCs) express neuron-specific proteins and functional neuron markers. efficiency CiNCs is unaffected the donor's age...
Here, we present an optical technique that can induce waves of calcium ion concentration in live biological cells. Ca2+ were induced by femtosecond pulsed-laser illumination. Living HeLa cells exposed to focused 140 fs pulses 780 nm wavelength at 30 mW average power. imaged fluorescence and observed propagate from the laser focal point inside cell. Photoinduced generation be performed any cell, improvement over previous mechanical or biochemical stimulation techniques.
Abstract —Although Ca 2+ waves in cardiac myocytes are regarded as arrhythmogenic substrates, their properties the heart situ poorly understood. On hypothesis that behave diversely and some of them influence function, we analyzed incidence, propagation velocity, intercellular at subepicardial myocardium fluo 3–loaded rat whole hearts using real-time laser scanning confocal microscopy. We classified into 3 types. In intact regions showing homogeneous transients under sinus rhythm (2 mmol/L...
We propose a multifocus scanning technique in second-harmonic-generation (SHG) microscopy which the SHG detection efficiency and image acquisition rate are improved by several tens of times compared with typical single-focus techniques. Because microlens array scanner, laser beam mode-locked Ti:sapphire is split into ~100 beamlets, same number foci formed specimen. Parallel enables us to observe real-time images The imaging properties usefulness microscope demonstrated living HeLa cells rat...
To assess the histologic reaction caused by biomaterial particles in different sizes around bone–implant interface, we examined ultra-high molecular weight polyethylene (UHMWPE, average diameter of 11 μm), UHMWPE (99 cobalt-chromium alloy (Co-Cr, 3.9 stainless steel (SUS316L, alumina ceramics (3.9 titanium (Ti, 3.5 Co-Cr (0.03 and Ti μm). After longitudinal groove on a polymethylmethacrylate plug was filled with one type particles, inserted into medullar canal distal end rabbit femurs,...