A5007429105- Peroxisome Proliferator-Activated Receptors
- RNA Research and Splicing
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Chromosomal and Genetic Variations
University of California, Santa Barbara
2023-2024
Abstract Development is generally viewed as one-way traffic of cell state transition from primitive to developmentally advanced states. However, molecular mechanisms that ensure the unidirectional fates remain largely unknown. Through exact transcription start site mapping, we report an evolutionarily conserved BTB domain-containing zinc finger protein, ZBTB12, a barrier for dedifferentiation human pluripotent stem cells (hPSCs). Single-cell RNA sequencing reveals ZBTB12 essential three germ...
Peroxisomes are membrane-bound organelles harboring metabolic enzymes. In humans, peroxisomes required for normal development, yet the genes regulating peroxisome function remain unclear. We performed a genome-wide CRISPRi screen to identify novel factors involved in peroxisomal homeostasis. found that inhibition of RNF146, an E3 ligase activated by poly(ADP-ribose), reduced import proteins into peroxisomes. RNF146-mediated loss depended on stabilization and activity poly(ADP-ribose)...
Abstract Peroxisomes are membrane-bound organelles harboring metabolic enzymes. In humans, peroxisomes required for normal development, yet the genes regulating peroxisome function remain unclear. We performed a genome-wide CRISPRi screen to identify novel factors involved in peroxisomal homeostasis. found that inhibition of RNF146, an E3 ligase activated by poly(ADP-ribose), reduced import proteins into peroxisomes. RNF146-mediated loss depended on stabilization and activity...