A study on RNA silencing of HPV E7 gene in human esophageal squamous cancer cell line EC9706

• DOI: 10.3760/cma.j.issn.1003-9279.2015.02.012
• Publication Date: 2015-04-30
• Authors: Shuang Wu; Mingkun Diao; Jintao Li; Hongwei Liu; Fan Li; Ping Gong; Rugang Zhong
• Keywords: Viability assay

Objective To explore on the impact of integrated HPV type 18 carcinogenesis esophageal squamous cancer cell line EC9706.

Methods A E7 gene silencing sequence was induced into pSUPER.retro.neo+ gfp vector to down regulate viral mRNA in EC9706 lines. Real time PCR and Western Blot were employed detect related genes both protein level respectively these The viability also detected by proliferate property.

Results The E6 regulated stable lines obtained. results showed that there a huge increase p27 which involved E7-cellular network. reduced expressed are factors enhance viability.

Conclusion This study proved could be pathogenetic factor EC9706, promising established basis RNA therapeutic practice cancer.

Key words: Papillomavirus, human; Genes; Esophageal neoplasms; Call