[Sodium cromoglycate attenuates experimental endometriosis in rats by regulating mast cells].

• DOI: 10.3785/j.issn.1008-9292.2015.05.07
• Publication Date: 2015-05-01
• Authors: Libo Zhu; Kaiqing Lin; Xinmei Zhang; Jun Lin
• Keywords: Tryptase; Intraperitoneal injection

To investigate the effect of sodium cromoglycate on experimental endometriosis in rats.Endometriosis model was established 36 unpregnant female SD rats by transplanting autologous fragments endometrium to inner surface abdominal wall. The endometriotic lesions were measured a second laparotomy 2 weeks after surgery. Then randomly divided into four groups (n=8 each group) receive intraperitoneal injection different doses for weeks: high-dose group (20 mg·kg⁻¹·d⁻¹); low-dose (10 negative control and blank group. animals sacrificed size measured. identified HE staining immunohistochemical keratin vimentin. total number mast cells their degranulation Toluidine blue staining; concentrations TNF-α serum enzyme linked immunosorbent assay; estradiol immunoassay; expression tryptase nerve growth factor (NGF) staining.The activated (MC) significantly lower than that (P<0.05), its ratio degranulation/total MC or (P<0.05). levels tissues those However, no significant difference NGF found among (P>0.05).Sodium can stabilize from degranulation, which may relieve clinical symptoms reducing levels.