Effects of Honghua preserved amniotic membrane on scar healing in experimental glaucoma surgery.

• DOI: 10.3980/j.issn.2222-3959.2014.02.07
• Publication Date: 2014-01-01
• Authors: Yi Shao; Yao Yu; Qiuping Liu; Jingming Li; Fei Dong; Xin Huang; Chong-Gang Pei; Ping Tu; Hen-Hui Li; Gui‐Ping Gao
• Keywords: Bleb (medicine); Glaucoma surgery

To investigate the efficacy and safety of Honghua preserved amniotic membrane (AM) for preventing scar formation filtering bleb in a rabbit model glaucoma trabeculectomy surgery.Totally 36 rabbits (36 eyes) were randomly divided into 3 groups: experimental group (ocular combination with AM transplantation), control surgery implantation), blank (single trabeculectomy). Clinical observations [including intraocular pressure (IOP), blebs complications], Masson-Trichrome staining, real-time quantitative reverse transcription-polymerase chain reaction (real-time PCR), Western blot performed on different time points (D1, D7, D14, D21 D56) after surgery.After operated 14d, there statistically significant differences compared to situation before operation (P<0.05), whereas no difference that among three groups (P>0.05). After 21d, IOP was lowest (P<0.05). There between On postoperative mean number fibroblasts significantly lower (40.6±10.2) those (54.4±10.8) (68.2±11.6) (P<0.05, respectively). The numbers macrophage respcitively versus P<0.05, Compared group, level transforming growth factor-β (TGF-β1) expression sclera conjunctival areas reduced protein mRNA but not these two (P>0.05).The trabeculectory can IOP, sustain functional filtration bleb, inhibit proliferation open filtrating pathway models.