A5088401995- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Nematode management and characterization studies
- CRISPR and Genetic Engineering
- Plant-Microbe Interactions and Immunity
- biodegradable polymer synthesis and properties
- Microbial Metabolic Engineering and Bioproduction
- Ion Transport and Channel Regulation
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Membrane-based Ion Separation Techniques
- Enzyme Structure and Function
- Plant tissue culture and regeneration
- Agronomic Practices and Intercropping Systems
- Genomics and Phylogenetic Studies
- Plant Molecular Biology Research
- Plant Pathogenic Bacteria Studies
- Bacterial Genetics and Biotechnology
- Plant Stress Responses and Tolerance
- Metalloenzymes and iron-sulfur proteins
- Animal Genetics and Reproduction
- Insect symbiosis and bacterial influences
- Turfgrass Adaptation and Management
- Antimicrobial Peptides and Activities
- Chromosomal and Genetic Variations
Instituto de Genética Veterinaria
2014-2024
Instituto de Biotecnología y Biología Molecular
2020-2024
National Agricultural Technology Institute
2010-2024
Instituto de Agrobiotecnología del Litoral
2022-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2023
Centro Científico Tecnológico - San Juan
2017-2022
National Institute of Industrial Technology
2013-2021
Instituto Nacional de Tecnologia
2013-2021
Centro Científico Tecnológico - Tucumán
2012
University of Buenos Aires
2006-2008
A main goal of biological nitrogen fixation research has been to expand the nitrogen-fixing ability major cereal crops. In this work, we demonstrate use efficient rhizobacterium Pseudomonas protegens Pf-5 X940 as a chassis engineer transfer fixed by BNF maize and wheat under non-gnotobiotic conditions. Inoculation with largely improved content biomass accumulation in both vegetative reproductive tissues, beneficial effect was positively associated high rates roots. 15 N isotope dilution...
In plant sexual reproduction, water and solute movement are tightly regulated, suggesting the involvement of aquaporins. We previously identified TIP5;1 TIP1;3 as only Arabidopsis aquaporin genes that selectively highly expressed in mature pollen, showed they can transport both urea when Xenopus oocytes. Here, we show has unusual characteristics, its activity is regulated by pH. Analysis a mutant version (TIP5;1-H131A) amino acid alignment with other aquaporins pH suggested conserved motif...
Nitrogen is the second most critical factor for crop production after water. In this study, beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using genes encoding nitrogenase of stutzeri A1501 via X940 cosmid. able grow in L medium without nitrogen, displayed high activity and released significant quantities ammonium medium. also showed constitutive expression enzymatic or nitrogen-free medium, suggesting a fixation. Similar Pf-5, putida, veronii...
Acetoacetyl-CoA thiolase (EC 2.3.1.9), also called II, condenses two molecules of acetyl-CoA to give acetoacetyl-CoA. This is the first enzymatic step in biosynthesis isoprenoids via mevalonate (MVA). In this work, II from alfalfa (MsAACT1) was identified and cloned. The activity experimentally demonstrated planta heterologous systems. condensation reaction by MsAACT1 proved be inhibited CoA suggesting a negative feedback regulation isoprenoid production. Real-time RT-PCR analysis indicated...
In flowers with dry stigmas, pollen development, pollination, and tube growth require spatial temporal regulation of water nutrient transport. To better understand the molecular mechanisms involved in reproductive processes, we characterized NIP4;1 NIP4;2, two pollen-specific aquaporins Arabidopsis thaliana. NIP4;2 are paralogs found exclusively angiosperm lineage. Although they have 84% amino acid identity, displayed different expression patterns. has low levels mature pollen, while peaks...
The production of antimicrobial peptides is a common defense strategy living cells against wide range pathogens. Plant snakin inhibit bacterial and fungal growth at extremely low concentrations. However, little known their molecular ecological characteristics, including origin, evolutionary equivalence, specific functions activity beneficial microbes. aim this study was to identify characterize snakin-1 from alfalfa (MsSN1).Phylogenetic analysis showed complete congruence between plant...
In Arabidopsis thaliana, light signals modulate the defences against bacteria. Here we show that perceived by LOV domain-regulated two-component system (Pst-Lov) of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) modulates virulence A. thaliana. Bioinformatic analysis and existence an episomal circular intermediate indicate locus encoding Pst-Lov is present in active genomic island acquired horizontal transfer. Strains mutated at showed enhanced growth on minimal medium leaves thaliana...
Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target is poorly understood. As first step elucidate mechanism, we screened mutation library Pseudomonas fluorescens Pf-5 in LB and agar medium supplemented with alfalfa snakin-1 (MsSN1). We identified three biofilm formation-related mutants that showed increased resistance MsSN1. Genetic, physiological...
Because its ability to acquire large amounts of nitrogen by symbiosis, tetraploid alfalfa is the main source vegetable proteins in meat and milk production systems temperate regions. Alfalfa cultivation also adds fixed soil, improving non-legumes crop rotation reducing use fertilizers derived from fossil fuel. Despite economic ecological relevance, genetics remains poorly understood, limiting development public elite germplasm. In this brief article, we reported high-efficiency mutagenesis...
Hydrogen peroxide (H2O2) transport by aquaporins (AQP) is a critical feature for cellular redox signaling. However, the H2O2 permeation mechanism through these channels remains poorly understood. Through functional assays, two Plasma membrane Intrinsic Protein (PIP) AQP from Medicago truncatula, MtPIP2;2 and MtPIP2;3 have been identified as pH-gated capable of facilitating both water (H2O) H2O2. Employing combination unbiased enhanced sampling molecular dynamics simulations, we investigated...
Festuca arundinacea Schreb., commonly known as tall fescue, is a major forage crop in temperate regions. Recently, molecular analysis of different accessions world germplasm collection fescue has demonstrated that it contains species from the genus and allowed their rapid classification into three morphotypes (Continental, Mediterranean Rhizomatous). In this study, we explored genetic diversity 161 29 countries, including 28 INTA (Argentina), by analyzing 15 polymorphic SSR markers capillary...
Pseudomonas sp. 14-3 accumulates polyhydroxybutyrate (PHB) from octanoate, but not glucose. To elucidate this unusual phenotype, genes responsible for the synthesis of PHB were cloned and analyzed. A polymerase gene (phaC) was found downstream coding a beta-ketothiolase (phaA), an acetoacetyl-coenzyme reductase (phaB) putative transcriptional regulator (phaR). All similar to pha several related species, differences observed in distal region phaA. Complementation with heterologous Azotobacter...
Recent advances at the molecular level are introducing a new scenario that needs to be integrated into analysis of plant hydraulic properties. Although it is not yet clear what extent this alters current proposal for circuit models, introduces insights when studying plants able easily overcome water restrictions. In context, our aim was explore adjustments in halotolerant model (Beta vulgaris) by coordination between root terms conductivity (Lpr) and shoot as reflected stomatal conductance...