A5052535152- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Microbial Community Ecology and Physiology
- Bacterial Genetics and Biotechnology
- Plant Pathogenic Bacteria Studies
- Genomics and Phylogenetic Studies
- Nematode management and characterization studies
- Bacterial biofilms and quorum sensing
- Agronomic Practices and Intercropping Systems
- Bacteriophages and microbial interactions
- Latin American Urban Studies
- RNA and protein synthesis mechanisms
- Mycorrhizal Fungi and Plant Interactions
- Medieval European Literature and History
- Photosynthetic Processes and Mechanisms
- Plant Parasitism and Resistance
- Protist diversity and phylogeny
- Insect symbiosis and bacterial influences
- Regional Development and Innovation
- Plant Disease Resistance and Genetics
- Microbial Natural Products and Biosynthesis
- Microbial Fuel Cells and Bioremediation
- biodegradable polymer synthesis and properties
- Plant tissue culture and regeneration
National University of Quilmes
2015-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2016-2024
Universidad Nacional de Hurlingham
2024
Centro Científico Tecnológico - San Juan
2018-2023
Aix-Marseille Université
2013
University Hospital Complex Of Vigo
2013
University Hospital Cologne
2013
Inserm
2013
University of Lausanne
2003-2008
Universidad Nacional de La Plata
1997
Soil bacteria that also form mutualistic symbioses in plants encounter two major levels of selection. One occurs during adaptation to and survival soil, the other concert with host plant speciation adaptation. Actinobacteria from genus Frankia are facultative symbionts N 2 -fixing root nodules on diverse globally distributed angiosperms “actinorhizal” symbioses. Three closely related clades sp. strains recognized; members each clade infect a subset among eight angiosperm families. We...
In Pseudomonas aeruginosa, the GacS/GacA two-component system positively controls quorum-sensing machinery and expression of extracellular products via two small regulatory RNAs, RsmY RsmZ. An rsmY rsmZ double mutant a gacA were similarly impaired in synthesis signal N-butanoyl-homoserine lactone, disulfide bond-forming enzyme DsbA, exoproducts hydrogen cyanide, pyocyanin, elastase, chitinase (ChiC), chitin-binding protein (CbpD). Both mutants showed increased swarming ability, azurin...
Summary In the plant‐beneficial soil bacterium and biocontrol model organism Pseudomonas fluorescens CHA0, GacS/GacA two‐component system upregulates production of factors, i.e. antifungal secondary metabolites extracellular enzymes, under conditions slow, non‐exponential growth. When activated, promotes transcription a small regulatory RNA (RsmZ), which sequesters RNA‐binding protein RsmA, translational regulator genes involved in biocontrol. The gene for second GacA‐regulated (RsmY) was...
ABSTRACT In soil ecosystems, bacteria must cope with predation activity, which is attributed mainly to protists. The development of antipredation strategies may help maintain higher populations and persist longer in the soil. We analyzed interaction between root-colonizing biocontrol strain Pseudomonas fluorescens CHA0 three different protist isolates (an amoeba, a flagellate, ciliate). produces set antibiotics, HCN, an exoprotease. observed that protists cannot grow on but can multiply...
A main goal of biological nitrogen fixation research has been to expand the nitrogen-fixing ability major cereal crops. In this work, we demonstrate use efficient rhizobacterium Pseudomonas protegens Pf-5 X940 as a chassis engineer transfer fixed by BNF maize and wheat under non-gnotobiotic conditions. Inoculation with largely improved content biomass accumulation in both vegetative reproductive tissues, beneficial effect was positively associated high rates roots. 15 N isotope dilution...
ABSTRACT In the plant-beneficial soil bacterium Pseudomonas fluorescens CHA0, production of biocontrol factors (antifungal secondary metabolites and exoenzymes) is controlled at a posttranscriptional level by GacS/GacA signal transduction pathway involving RNA-binding protein RsmA as key regulatory element. This assumed to bind ribosome-binding site target mRNAs block their translation. RsmA-mediated repression relieved end exponential growth two GacS/GacA-controlled RNAs RsmY RsmZ, which...
The riboregulator RsmY of Pseudomonas fluorescens strain CHA0 is an example small regulatory RNAs belonging to the global Rsm/Csr systems controlling diverse cellular processes such as glycogen accumulation, motility, or formation extracellular products in various bacteria. By binding multiple molecules protein RsmA, relieves negative effect RsmA on translation several target genes involved biocontrol properties CHA0. and functionally related riboregulators have repeated GGA motifs predicted...
In Pseudomonas fluorescens biocontrol strain CHA0, the two-component system GacS/GacA positively controls synthesis of extracellular products such as hydrogen cyanide, protease, and 2,4-diacetylphloroglucinol, by upregulating transcription small regulatory RNAs which relieve RsmA-mediated translational repression target genes. The expression stress sigma factor σS (RpoS) was controlled GacA negatively RsmA. By comparison with wild-type both a gacS an rpoS null mutant were more sensitive to...
Small RNAs (sRNAs) are widespread among bacteria and have diverse regulatory roles. Most of these sRNAs been discovered by a combination computational experimental methods. In Pseudomonas aeruginosa, ubiquitous Gram-negative bacterium opportunistic human pathogen, the GacS/GacA two-component system positively controls transcription two (RsmY, RsmZ), which crucial for expression genes involved in virulence. biocontrol fluorescens CHA0, three GacA-controlled (RsmX, RsmY, RsmZ) regulate...
Small non-coding RNAs (sRNAs) have emerged as ubiquitous regulatory elements in bacteria and other life domains. However, few sRNAs been identified outside several well-studied species of gamma-proteobacteria thus relatively little is known about the role RNA-mediated regulation most bacterial genera. Here we conducted a computational prediction putative sRNA genes intergenic regions (IgRs) symbiotic α-proteobacterium S. meliloti 1021 experimentally confirmed expression dozens these...
The functioning of plant-associated bacteria is strongly influenced by their interaction with other organisms. For instance, upregulate the production secondary metabolites in presence protozoa and we hypothesised that this may contribute to plant health. Here, tested if effect beneficial pseudomonads on wheat growth health modified co-inoculation bacterivorous amoeba Acanthamoeba castellanii. We assessed effects absence root pathogen Pythium ultimum. In amoebae, bacterial isolates had few...
Plant-growth promotion has been linked to the Pseudomonas genus since beginning of this research field. In work, we mined genome an Argentinean isolate recently described species P. donghuensis. Strain SVBP6, isolated from bulk soil agricultural plot, showed a broad antifungal activity and several other plant-probiotic activities. As described, it seems like some plant-growth promoting (PGP) traits do not belong classical pseudomonads toolbox, decide explore SVBP6 via bioinformatic approach....
Summary Pseudomonas donghuensis strain SVBP6, an isolate from agricultural plot in Argentina, displays a broad‐spectrum and diffusible antifungal activity, which requires functional gacS gene but could not be ascribed yet to known secondary metabolites typical of biocontrol species. Here, we report that Tn 5 mutagenesis allowed the identification cluster involved both fungal antagonism production soluble tropolonoid compound. The ethyl acetate extract culture supernatant showed...
Gene expression is adjusted according to cellular needs through a combination of mechanisms acting at different layers the flow genetic information. At posttranscriptional level, RNA-binding proteins are key factors controlling fate nascent and mature mRNAs. Among them, members CsrA family small dimeric with heterogeneous distribution across bacterial tree life, that act as global regulators gene because they recognize characteristic sequence/structural motifs (short hairpins GGA triplets in...
Root-colonizing bacteria can support plant growth and help fend off pathogens. It is clear that such benefit from plant-derived carbon, but it remains ambiguous why they invest in plant-beneficial traits. We suggest selection via protist predation contributes to recruitment of traits rhizosphere bacteria. To this end, we examined the extent which bacterial associated with pathogen inhibition coincide resistance predation. investigated a collection Pseudomonas spp. against range...
Riboregulation stands for RNA-based control of gene expression. In bacteria, small non-coding RNAs (sRNAs) are a major class riboregulatory elements, most which act at the post-transcriptional level by base-pairing target mRNA genes. The RNA chaperone Hfq facilitates antisense interactions between mRNAs and regulatory sRNAs, thus influencing stability and/or translation rate. α-proteobacterium Sinorhizobium meliloti strain 2011, identification detection multiple sRNAs genes broadly...
Pseudomonas sp. strain 1008 was isolated from the rhizosphere of field grown wheat plants at tillering stage in an agricultural plot near Pergamino city, Argentina. Based on its vitro phosphate solubilizing capacity and production IAA, formulated as inoculant for bacterization seeds subjected to multiple assays within period 2010-2017. showed a robust positive impact grain yield (+8% average) across number campaigns, soil properties, seed genotypes, with no significant influence simultaneous...
The time course of initiation and development root nodules was investigated in the South American actinorhizal shrub Discaria trinervis (Rhamnaceae). A local strain Frankia (BCU110501) which isolated from D. nodules, used as inoculum. Inoculated seedlings were periodically studied under light microscope after clearing with aqueous NaClO. In parallel, semithin ultrathin sections analysed by electron microscopy. Infection BCU110501 involved intercellular penetration among epidermal cortical...
Summary After nitrogen (N), phosphorus (P) is the nutrient that most limits plant productivity. The role of P on growth and root nodulation was studied in actinorhizal symbiosis between Discaria trinervis Frankia , an intercellular infected N 2 –fixing association. Growth, content (N P) were analysed symbiotic plants receiving different supplies solutions. relative requirement for P‐deficient plants. Nodule initiation less impaired than general by low P. However, nodule to a greater extent...