A5089948498- Microbial Community Ecology and Physiology
- Plant-Microbe Interactions and Immunity
- Mycorrhizal Fungi and Plant Interactions
- Legume Nitrogen Fixing Symbiosis
- Soil Carbon and Nitrogen Dynamics
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Wastewater Treatment and Nitrogen Removal
- Nematode management and characterization studies
- Gut microbiota and health
- Plant and animal studies
- Ecology and Vegetation Dynamics Studies
- Peatlands and Wetlands Ecology
- Environmental DNA in Biodiversity Studies
- Forest Ecology and Biodiversity Studies
- Plant tissue culture and regeneration
- Polar Research and Ecology
- Plant Parasitism and Resistance
- Protist diversity and phylogeny
- Plant responses to elevated CO2
- Plant Disease Resistance and Genetics
- Coastal wetland ecosystem dynamics
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Molecular Biology Techniques and Applications
Utrecht University
2015-2024
Nanjing Agricultural University
2018-2023
Netherlands Institute of Ecology
2005-2015
University of Amsterdam
2007-2015
Vrije Universiteit Amsterdam
2006-2013
Royal Netherlands Academy of Arts and Sciences
2001
Yale University
1994-1995
Plants and their arbuscular mycorrhizal fungal symbionts interact in complex underground networks involving multiple partners. This increases the potential for exploitation defection by individuals, raising question of how partners maintain a fair, two-way transfer resources. We manipulated cooperation plants to show that can detect, discriminate, reward best with more carbohydrates. In turn, enforce increasing nutrient only those roots providing On basis these observations we conclude that,...
Denaturing gradient gel electrophoresis (DGGE) is a powerful and convenient tool for analyzing the sequence diversity of complex natural microbial populations. DGGE was evaluated identification ammonia oxidizers beta subdivision Proteobacteria based on mobility PCR-amplified 16S rDNA fragments analysis mixtures PCR products from this group generated by selective DNA extracted coastal sand dunes. Degenerate primers, CTO189f-GC CTO654r, incorporating 5' GC clamp, were designed to amplify...
Plants are capable of building up beneficial rhizosphere communities as is evidenced by disease-suppressive soils. However, it not known how and why soil bacterial impacted plant exposure to foliar pathogens if such responses might improve performance in the presence pathogen. Here, we conditioned growing multiple generations (five) Arabidopsis thaliana inoculated aboveground with Pseudomonas syringae pv tomato (Pst) same soil. We then examined a subsequent generation (sixth) grown...
Soil microbiome composition and functioning determine the outcome of plant-pathogen interactions under natural field conditions.
Rising atmospheric CO 2 levels are predicted to have major consequences on carbon cycling and the functioning of terrestrial ecosystems. Increased photosynthetic activity is expected, especially for C-3 plants, thereby influencing vegetation dynamics; however, little known about path fixed into soil-borne communities resulting feedbacks ecosystem function. Here, we examine how arbuscular mycorrhizal fungi (AMF) act as a conduit in transfer between plants soil elevated modulates belowground...
To gain insight into the factors driving structure of bacterial communities in soil, we applied real-time PCR, PCR-denaturing gradient gel electrophoreses, and phylogenetic microarray approaches targeting 16S rRNA gene across a range different land usages Netherlands. We observed that main differences were not related to land-use type, but rather soil factors. An exception was community pine forest soils (PFS), which clearly from all other sites. PFS had lowest abundance, numbers operational...
• The impact of various agricultural practices on soil biodiversity and, in particular, arbuscular mycorrhizal fungi (AMF), is still poorly understood, although AMF can provide benefit to plants and ecosystems. Here, we tested whether organic farming enhances diversity communities from organically managed fields are more similar those species-rich grasslands or conventionally fields. To address this issue, the community composition was assessed 26 arable (13 pairs fields) five semi-natural...
Abstract Background Plant diseases caused by fungal pathogen result in a substantial economic impact on the global food and fruit industry. Application of organic fertilizers supplemented with biocontrol microorganisms ( i.e. bioorganic fertilizers) has been shown to improve resistance against plant pathogens at least part due impacts structure function resident soil microbiome. However, it remains unclear whether such improvements are driven specific action microbial inoculants, populations...
Although interactions between plants and microbes at the plant-soil interface are known to be important for plant nutrient acquisition, relatively little is about how root exudates contribute exchange over course of development. In this study, from slow- fast-growing stages Arabidopsis thaliana were collected, chemically analysed then applied a sandy nutrient-depleted soil. We tracked impacts these on soil bacterial communities, nutrients (ammonium, nitrate, available phosphorus potassium)...
Abstract Understanding how communities assemble is a central goal of ecology. This particularly relevant for arbuscular mycorrhizal fungi (AMF), because the community composition these beneficial plant symbionts influences important ecosystem processes. Moreover, AMF may be used as sensitive indicators ecological soil quality if they respond to environmental variation in predictable way. Here, we use molecular profiling technique (T‐RFLP 25S rRNA gene fragments) test which factors determine...
Abstract Background Plant health is intimately influenced by the rhizosphere microbiome, a complex assembly of organisms that changes markedly across plant growth. However, most microbiome research has focused on fractions this particularly bacteria and fungi. It remains unknown how other microbial components, especially key predators—protists—are linked to health. Here, we investigated holistic including bacteria, eukaryotes (fungi protists), as well functional metabolism genes. We these...
Plants are known to influence belowground microbial community structure along their roots, but the impacts of plant species richness and functional group (FG) identity on communities in bulk soil still not well understood. Here, we used 454-pyrosequencing analyse composition a long-term biodiversity experiment at Jena, Germany. We examined responses bacteria, fungi, archaea, protists (communities varying from 1 60 sown species) FG (grasses, legumes, small herbs, tall herbs) soil....