A5086721510- Cardiovascular and Diving-Related Complications
- Metabolism and Genetic Disorders
- Diet and metabolism studies
- Respiratory Support and Mechanisms
- High Altitude and Hypoxia
- Drug-Induced Hepatotoxicity and Protection
- Gastroesophageal reflux and treatments
- Amyotrophic Lateral Sclerosis Research
- Cardiac Arrest and Resuscitation
- Biochemical Acid Research Studies
- Neuroscience of respiration and sleep
- Advanced Chemical Sensor Technologies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Zebrafish Biomedical Research Applications
- Airway Management and Intubation Techniques
- Analytical Chemistry and Sensors
- Adipose Tissue and Metabolism
- Pharmacological Effects of Natural Compounds
- Bladder and Urothelial Cancer Treatments
- Neurological Disorders and Treatments
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Medical Research and Practices
- Hormonal and reproductive studies
- Water Treatment and Disinfection
- Neuroscience and Neuropharmacology Research
University of Arizona
2024
University at Buffalo, State University of New York
2013-2017
University of South Florida
2012-2015
Central nervous system oxygen toxicity (CNS-OT) seizures occur with little or no warning, and effective mitigation strategy has been identified. Ketogenic diets (KD) elevate blood ketones have successfully treated drug-resistant epilepsy. We hypothesized that a ketone ester given orally as R,S-1,3-butanediol acetoacetate diester (BD-AcAc(2)) would delay CNS-OT in rats breathing hyperbaric (HBO(2)). Adult male (n = 60) were implanted radiotelemetry units to measure electroencephalogram (EEG)....
Amyotrophic Lateral Sclerosis (ALS), also known as Lou Gehrig's disease, is a neurodegenerative disorder of motor neurons causing progressive muscle weakness, paralysis, and eventual death from respiratory failure. There currently no cure or effective treatment for ALS. Besides neuron degeneration, ALS associated with impaired energy metabolism, which pathophysiologically linked to mitochondrial dysfunction glutamate excitotoxicity. The Deanna Protocol (DP) metabolic therapy that has been...
Submersion and increased pressure (depth) characterize the diving environment may independently increase demand on respiratory system. To quantify changes in mechanics, this study employed a unique protocol techniques to measure, hyperbaric chamber, inspiratory expiratory alveolar pressures (interrupter technique), resistance airways (RawI RawE, esophageal balloon nitric oxide elimination (thought correlate with Raw), mechanical power of breathing, total energy cost ventilation. Eight...
Abstract Tonic-clonic seizures typify central nervous system oxygen toxicity (CNS-OT) in humans and animals exposed to high levels of oxygen, as are encountered during scuba diving. We previously demonstrated that doses pseudoephedrine (PSE) decrease the latency seizure (LS) for CNS-OT young male rats. This study investigated whether female rats respond similarly PSE hyperbaric (HBO). implanted 60 virgin stock (VS) 54 former breeder (FB) with radio-telemetry devices measured brain electrical...
CNS‐OT limits the use of hyperbaric O 2 (HBO ) clinically and in military diving. We studied effects preconditioning (PCs) gas mixtures dive/seizure history on latency to seizure (LS) 118 adult rats implanted with a radio‐transmitter record EEGs. After ≥1 wk recovery, underwent: a) hyperoxic PC at 1‐ 2ATA pure ; b) normoxic using Heliox/Nitrox; c) air 1ATA (control) or 2ATA. All protocols were conducted 3x, followed 24hr after last treatment by one dive 5ATA seizure. Hyperoxic did not...
We previously described the effect of KEs, non‐ionized precursors ketone bodies, in delaying seizures rats breathing HBO 2 at 5 ATA. hypothesize that oral administration specific KEs (esters acetoacetate) will cause a rapid and sustained ketosis confers neuroprotection against CNS‐OT rats. Male (n = 53) were implanted with radio‐transmitters to measure diaphragmatic electromyogram (dEMG), ECG EEG. ≥ 7 days post‐surgery, fasted for 12h administered intragastrically 1 (10g/kg), including...
To evaluate trends in DMT use individuals with POMS and determine if there were demographic factors that influence utilization.
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder affecting motor neurons and characterized by progressive muscle weakness, paralysis, and, eventually, death. There no cure for ALS. This metabolic profiling study was conducted to determine the impacts of multi‐component experimental nutritional regimen known as Deanna Protocol (DP) gain insights into possible mechanisms which this diet reported help slow progression In 30 sample SOD1 G93A mice were fed 6 weeks standard...
Pseudoephedrine (PSE) salts (hydrochloride and sulfate) are commonly used as a decongestant by scuba divers. Anecdotal evidence collected Divers Alert Network (DAN) suggests that use of PSE prior to making high dose O 2 dive (Nitrox or rebreather using pure ) increases the risk CNS oxygen toxicity (CNS‐OT; i.e., seizures). We hypothesized doses reduces latency seizure (LS) in unanesthetized rats breathing lethal HBO (5 ATA). Adult male were implanted with radio‐transmitter for recording EEG,...
In diseases where glucose utilization is impaired ketone bodies and TCA cycle intermediates serve as alternative fuel. Since these are linked pathophysiologically to mitochondrial function, we hypothesized that nutritional ketosis induced by ketogenic precursors or intermediate supplements promote biogenesis preservation of existing mitochondria. Exogenous supplementation was used in Sprague Dawley (SD) rats SOD1 G93A mice. SD rats, ester salt‐MCT oil mixture gavaged daily, whereas the...
We previously reported that inhaling 10% CO2 increased efflux of CO2‐laden gas from cats’ stomachs. Anesthetized cats were surgically instrumented: we measured electromyograms (EMGs) respiratory and oropharyngeal muscles, including crural diaphragm (CD) upper esophageal sphincter (UES), as well tracheal flow composition. Animals exposed to six, 10‐min bouts breathing separated by 10 min air breathing. All animals produced gastric expelled it during cough, but half failed expel normal...
ABSTRACT Water covers over 70% of the earth, has varying depths and temperatures contains much earth's resources. Head‐out water immersion (HOWI) or submersion at various (diving) in thermoneutral (TN) temperature elicits profound cardiorespiratory, endocrine, renal responses. The translocation blood into thorax elevation plasma volume by autotransfusion fluid from cells to vascular compartment lead increased cardiac stroke output there is a hyperperfusion some tissues. Pulmonary artery...
Commonly, PSE salts are utilized by scuba divers as nasal decongestants. Anecdotal reports from Divers Alert Network (DAN) suggest that taking prior to a deep dive while breathing pure O 2 (i.e., Nitrox or using rebreather) increases the risk for CNS‐OT. We hypothesized high doses of reduce latency seizure (LS) in male and female Sprague‐Dawley rats lethal dose HBO (5ATA). 63 were implanted with radio‐transmitters recording EEG, body temperature (BT) activity. After ≥7 days, 2hr before...
We recently described gastric ventilation, in which hypercapnia stimulates acid and bicarbonate secretion the lumen, where it produces CO 2 that may be vented through esophagus. This study attempts to correlate respiratory responses . Rats were implanted with catheters. A pH probe sat stomach while animals a head‐body plethysmograph. Animals breathed air +5% awake (n=4) anesthetized (n=1). While awake, decreased (mean ± SE 3.52 0.35 air; 3.42 ±0.39 ) tidal volume (V T , 1.12 0.1 ml 0.83 but...
We previously described gastric ventilation, a phenomenon in which hypercapnia stimulates acid secretion. In the present study, we tested effects of following on secretion: normobaric normoxia (NN) hypobaric hypoxia (hh), (Nh), hyperbaric hyperoxia (HH), (HC), and (HN). Two rats implanted with catheters have completed experiments; 1 experiments. experiments, minute ventilation (V E ) was 74.4 ± 5.7 ml/min at NN (mean SE) increased as expected to 81.6 35.5 hh 76.1 8.6 Nh; pH appeared...