Cancer cells express an abnormal metabolism characterized by increased glucose consumption owing to genetic mutations and mitochondrial dysfunction. Previous studies indicate that unlike healthy tissues, cancer are unable effectively use ketone bodies for energy. Furthermore, ketones inhibit the proliferation viability of cultured tumor cells. As Warburg effect is especially prominent in metastatic cells, we hypothesized dietary supplementation would progression vivo. Proliferation were...

Introduction Abnormal cancer metabolism creates a glycolytic-dependency which can be exploited by lowering glucose availability to the tumor. The ketogenic diet (KD) is low carbohydrate, high fat decreases blood and elevates ketones has been shown slow progression in animals humans. tumor vasculature hypoxic pockets promote further increase of cancers. Hyperbaric oxygen therapy (HBO2T) saturates tumors with oxygen, reversing promoting effects hypoxia. Since these non-toxic therapies exploit...

Nutritional ketosis induced by the ketogenic diet (KD) has therapeutic applications for many disease states. We hypothesized that oral administration of exogenous ketone supplements could produce sustained nutritional (>0.5 mM) without carbohydrate restriction.We tested effects 28-day five on blood glucose, ketones, and lipids in male Sprague-Dawley rats. The included: 1,3-butanediol (BD), a sodium/potassium β-hydroxybutyrate (βHB) mineral salt (BMS), medium chain triglyceride oil (MCT), BMS...

Nutritional ketosis has been proven effective for seizure disorders and other neurological disorders. The focus of this study was to determine the effects ketone supplementation on anxiety-related behavior in Sprague-Dawley (SPD) Wistar Albino Glaxo/Rijswijk (WAG/Rij) rats. We tested exogenous supplements added food fed chronically 83 days SPD rats administered sub-chronically 7 both rat models by daily intragastric gavage bolus followed assessment anxiety measures elevated plus maze (EPM)....

Abstract Background Cancer Anorexia Cachexia Syndrome (CACS) is a distinct atrophy disease negatively influencing multiple aspects of clinical care and patient quality life. Although it directly causes 20% all cancer‐related deaths, there are currently no model systems that encompass the entire multifaceted syndrome, nor any effective therapeutic treatments. Methods A novel systemic metastasis was evaluated for comprehensive CACS (metastasis, skeletal muscle adipose tissue wasting,...

Abstract Athletes, clinicians, and practitioners are increasingly interested in the proposed performance therapeutic benefits of nutritional ketosis (NK). NK is best operationally defined as a nutritionally induced metabolic state resulting blood β-hydroxybutyrate concentrations ≥0.5 mM. Most tissues readily metabolize ketone bodies (KBs), KBs turn regulate metabolism signaling both systemic tissue-specific manner. During fasting, starvation, or ketogenic diets, endogenous synthesis...

The Warburg effect and tumor hypoxia underlie a unique cancer metabolic phenotype characterized by glucose dependency aerobic fermentation. We previously showed that two non-toxic therapies - the ketogenic diet with concurrent hyperbaric oxygen (KD+HBOT) dietary ketone supplementation could increase survival time in VM-M3 mouse model of metastatic cancer. hypothesized combining these provide an even greater therapeutic benefit this model. Mice receiving combination therapy demonstrated...

Amyotrophic Lateral Sclerosis (ALS), also known as Lou Gehrig's disease, is a neurodegenerative disorder of motor neurons causing progressive muscle weakness, paralysis, and eventual death from respiratory failure. There currently no cure or effective treatment for ALS. Besides neuron degeneration, ALS associated with impaired energy metabolism, which pathophysiologically linked to mitochondrial dysfunction glutamate excitotoxicity. The Deanna Protocol (DP) metabolic therapy that has been...

Introduction Reliable assessments of learning ability in preclinical models are essential for studying neurodegenerative, developmental, and inflammatory disorders. However, many inbred strains mice present background pathologies that interfere with traditional tests. The C57BL/6 J mouse, a widely used laboratory strain, sporadically develops auditory visual impairments complicate interpretation. In this study, we establish an olfaction-based learned preference protocol designed to evaluate...

The robust glycolytic metabolism of glioblastoma multiforme (GBM) has proven them susceptible to increases in oxidative induced by the pyruvate mimetic dichloroacetate (DCA). Recent reports demonstrate that anti-diabetic drug metformin enhances damaging stress associated with DCA treatment cancer cells. We sought elucidate role metformin's reported activity as a mitochondrial complex I inhibitor enhancement cytotoxicity VM-M3 GBM Metformin potentiated DCA-induced superoxide production, which...

Heavy reliance on glucose metabolism and a reduced capacity to use ketone bodies makes glioblastoma (GBM) promising candidate for ketone-based therapies. Ketogenic diet (KD) is well-known its effects in controlling tumor growth GBM. Moreover, synthetic ester (KE) has demonstrated increase blood levels enhance animal survival metastatic VM-M3 murine model. Here, we compared the efficacy of KE-supplemented Atkins-type (ATD-KE) classic KD progression enhancing clinically relevant orthotopic...

Objective: Exogenous ketones may provide therapeutic benefit in treatment of obesity. Administration the ketone ester (KE) R,S-1,3-butanediol acetoacetate diester (BD-AcAc2) decreases body weight mice, but effects on energy balance have not been extensively characterized. The purpose this investigation was to explore concentration-dependent BD-AcAc2 intake and expenditure mice. Methods: Forty-two male C57BL/6J mice were randomly assigned one seven isocaloric diets (n=6 per group): 1) Control...

The aim of this study was to examine the effects a ketone ester (KE)-supplemented diet on energy expenditure (EE) and adiposity in mice housed at 23 °C versus thermoneutrality (30 °C), which sympathetic nervous system activity is diminished.Thirty-two 10-week-old male C57BL/6J were assigned 1 4 groups (n = 8 per group): 30% KE + (KE23), control (CON) (CON23), 30 (KE30), or CON (CON30). pair-fed average intake consuming (ad libitum) for weeks. Body composition components balance measured...