A5074859611- Muscle metabolism and nutrition
- Sports Performance and Training
- Muscle Physiology and Disorders
- Exercise and Physiological Responses
- Cardiovascular and exercise physiology
- Chromosomal and Genetic Variations
- Nutrition and Health in Aging
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- PI3K/AKT/mTOR signaling in cancer
- Genetic Neurodegenerative Diseases
- Diet and metabolism studies
- Genetics and Physical Performance
- Adipose Tissue and Metabolism
- Vitamin D Research Studies
- Muscle activation and electromyography studies
- Vitamin C and Antioxidants Research
- Occupational Health and Performance
- Body Composition Measurement Techniques
- Protein Kinase Regulation and GTPase Signaling
- Advanced biosensing and bioanalysis techniques
- Sleep and related disorders
- Sports injuries and prevention
- Physical Activity and Health
- Phytoestrogen effects and research
Penn State Milton S. Hershey Medical Center
2021-2024
University of Colorado Anschutz Medical Campus
2024
Auburn University
2017-2022
Applied Sciences Laboratory (United States)
2018-2022
Pennsylvania State University
2020-2022
James Madison University
2016-2018
BHP (Australia)
2004
Adopting low carbohydrate, ketogenic diets remains a controversial issue for individuals who resistance train given that this form of dieting has been speculated to reduce skeletal muscle glycogen levels and stifle anabolism. We sought characterize the effects 12-week diet (KD) on body composition, metabolic, performance parameters in participants trained recreationally at local CrossFit facility. Twelve (nine males three females, 31 ± 2 years age, 80.3 5.1 kg mass, 22.9 2.3% fat, 1.37 back...
We sought to determine the effects of L-leucine (LEU) or different protein supplements standardized LEU (~3.0 g/serving) on changes in body composition, strength, and histological attributes skeletal muscle adipose tissue. Seventy-five untrained, college-aged males (mean ± standard error mean (SE); age = 21 1 years, mass 79.2 0.3 kg) were randomly assigned an isocaloric, lipid-, organoleptically-matched maltodextrin placebo (PLA, n 15), (n 14), whey concentrate (WPC, 17), hydrolysate (WPH,...
We sought to identify biomarkers which delineated individual hypertrophic responses resistance training. Untrained, college-aged males engaged in full-body training (3 d/wk) for 12 weeks. Body composition via dual x-ray absorptiometry (DXA), vastus lateralis (VL) thickness ultrasound, blood, VL muscle biopsies, and three-repetition maximum (3-RM) squat strength were obtained prior (PRE) following (POST) weeks of K-means cluster analysis based on changes identified LOW [n = 17; change...
Abstract Background Cancer Anorexia Cachexia Syndrome (CACS) is a distinct atrophy disease negatively influencing multiple aspects of clinical care and patient quality life. Although it directly causes 20% all cancer‐related deaths, there are currently no model systems that encompass the entire multifaceted syndrome, nor any effective therapeutic treatments. Methods A novel systemic metastasis was evaluated for comprehensive CACS (metastasis, skeletal muscle adipose tissue wasting,...
Cellular adaptations that occur during skeletal muscle hypertrophy in response to high-volume resistance training are not well-characterized. Therefore, we sought explore how actin, myosin, sarcoplasmic protein, mitochondrial, and glycogen concentrations were altered individuals exhibited mean fiber cross-sectional area (fCSA) following 6 weeks of training. Thirty previously resistance-trained, college-aged males (mean ± standard deviation: 21±2 years, 5±3 years) had vastus lateralis (VL)...
The goal of this project was to examine the influence a single night sleep restriction following heavy exercise on cycling time-trial (TT) performance and skeletal muscle function in morning. Seven recreational cyclists (age, 24 ± 7 years; peak oxygen consumption, 62 4 mL·kg −1 ·min ) completed 2 phases, each comprising evening (EX1) next-morning (EX2) sessions. EX1 EX2 were separated by an assigned condition: full rest (CON; 7.1 0.3 h sleep) or through early waking (SR; 2.4 0.2 h)....
Limited evidence exists regarding differentially expressed biomarkers between previously-trained low versus high hypertrophic responders in response to resistance training. Herein, 30 college-aged males (training age 5 ± 3 years; mean SD) partook 6 weeks of high-volume Body composition, right leg vastus lateralis (VL) biopsies, and blood were obtained prior training (PRE) at the 3-week (W3) 6-week time points (W6). The 10 lowest (LOW) highest (HIGH) clustered based upon a composite...
We sought to examine how 12 weeks of resistance exercise training (RET) affected skeletal muscle myofibrillar and sarcoplasmic protein levels along with markers mitochondrial physiology in high versus low anabolic responders.Untrained college-aged males were classified as responders the top 25th percentile (high-response cluster (HI); n = 13, dual x-ray absorptiometry total body mass change (Δ) +3.1 ± 0.3 kg, Δ vastus lateralis (VL) thickness +0.59 0.05 cm, fiber cross sectional area +1,426...
Recent evidence suggests that resistance training with light or heavy loads to failure results in similar adaptations. Herein, we compared how both modalities affect the molecular, neuromuscular, and recovery responses following exercise. Resistance-trained males (mean ± SE: 22 2 years, 84.8 9.0 kg, 1.79 0.06 m; n = 15) performed a crossover design of four sets leg extensor exercise at 30% (light RE) 80% (heavy one repetition maximum (1RM) failure, RE 1 week later. Surface electromyography...
We examined hypertrophic outcomes of weekly graded whey protein dosing (GWP) vs. (WP) or maltodextrin (MALTO) dosed once daily during 6 weeks high-volume resistance training (RT). College-aged resistance-trained males (training age = 5 ± 3 years; mean SD) performed RT wherein frequency was d/week and each session involved 2 upper- lower-body exercises (10 repetitions/set). Volume increased from 10 sets/exercise (week 1) to 32 6), which is the highest volume investigated in this timeframe....
The current study investigated how bovine milk exosomes affected rotarod performance and biomarkers of skeletal muscle physiology in young, growing rats. Twenty-eight-day Fisher 344 rats were provided an AIN-93G-based diet for four weeks that either remained unadulterated (exosome RNA-sufficient (ERS; n=12)) or was sonicated RNA-depleted (ERD; n=12)). Prior to (PRE) on the last day intervention (POST), animals tested maximal performance. Following feeding period, gastrocnemius analyzed at...
Resistance training generally increases skeletal muscle hypertrophy, whereas aging is associated with a loss in mass. Interestingly, select studies suggest that aging, as well resistance training, may lead to reduction the abundance of myofibrillar (or contractile) protein (per mg tissue). Proteomic interrogations have also demonstrated weeks months appreciable alterations proteome. Given this evidence, purpose small pilot study was examine total sarcoplasmic concentrations wet muscle) from...
Several published protocols exist for isolating contractile or myofibrillar (MF) proteins from skeletal muscle, however, achieving complete resuspension of the myofibril pellet can be technically challenging. We performed several previously MF isolation methods with intent determining which method was most suitable protein and solubilization. Here, we provide an optimized protocol to isolate sarcoplasmic solubilized fractions mammalian muscle downstream assays.
While high-load resistance training increases muscle hypertrophy, the intramuscular protein responses to this form of remains largely unknown. In current study, recreationally resistance-trained college-aged males (N=15; mean±SD: 23±3 years old, 6±5 training) performed full-body, low-volume, (68%-90% one repetition maximum (1RM)) over 10 weeks. Back squat strength testing, body composition and a vastus lateralis biopsy were before (PRE) 72 hours after 10-week program (POST). Fiber...
Introduction: Amino acid transporters are essential for cellular amino transport and promoting protein synthesis. While previous literature has demonstrated the association of synthesis following acute resistance exercise supplementation, chronic effect supplementation on is unknown. The purpose herein was to determine if metabolic enzymes were related skeletal muscle hypertrophy training with different nutritional strategies. Methods: 43 college-aged males separated into a maltodextrin...
We find no effect of follicle-stimulating hormone (FSH) on energy balance using a novel model in which rats are ovariectomized, subjected to gonadotropin-releasing antagonism, and systematically given back FSH by osmotic pump. However, tissue-specific effects adipose tissue liver were observed this study. These include unique transcriptomic signatures induced the stark reduction hepatic triglyceride accumulation.
We investigated the effects of whey protein (WP) supplementation on body composition and physical performance in soldiers participating Army Initial Entry Training (IET). Sixty-nine, male United States volunteered for with either twice daily (WP, 77 g/day protein, ~580 kcal/day; n = 34, age 19 ± 1 year, height 173 6 cm, weight 73.4 12.7 kg) or energy-matched carbohydrate (CHO) drinks (CHO, 127 carbohydrate, 35, 5 72.3 10.9 eight weeks during IET. Physical was evaluated using Fitness Test two...
The long interspersed nuclear element-1 (L1) is a retrotransposon that constitutes 17% of the human genome and associated with various diseases aging. Estimates suggest ~100 L1 copies are capable copying pasting into other regions genome. Herein, we examined if skeletal muscle markers affected by aging or an acute bout cycling exercise in humans. Apparently healthy younger (23 ± 3 y, n = 15) older participants (58 8 donated vastus lateralis biopsy before 1 h (PRE) at ~70% heart rate reserve....
We sought to determine whether age-related gastrocnemius muscle mass loss was associated with parallel decrements in androgen receptor (AR) or select Wnt signaling markers. To test this hypothesis, serum-free and total testosterone (TEST) AR markers were analyzed male Fischer 344 rats that 3, 6, 12, 18, 24 mo (mo) old ( n = 9 per group). Free TEST greatest 6 rats, protein Wnt5 levels linearly declined aging. There associations between relative r 0.395, P 0.007) as well (r 0.670, < 0.001)....
Introduction: Protein supplementation is proposed to promote recovery and adaptation following endurance exercise. While prior literature demonstrates improved performance when supplementing protein during or exercise, chronic research limited. Methods: Runners (VO2peak = 53.6 ± 8.9 ml/kg/min) were counter-balanced into a placebo group (PLA; n 8) (PRO; 9) based on sex VO2peak, underwent 10 weeks of progressive training. Prior training, body composition, blood cell differentials, non-invasive...
Hindlimb unloading causes significant skeletal muscle atrophy by adversely affecting the balance between protein synthesis and breakdown. This study demonstrates a more complete time course for changes in biomarkers associated with breakdown investigates anabolic resistance to an stimulus following hindlimb unloading. These data concert information from other studies provide basis designing future experiments optimally interrogate desired cellular biomarker or pathway.
We sought to determine if a pre-workout supplement (PWS), containing multiple ingredients thought enhance blood flow, increases hyperemia associated with resistance training compared placebo (PBO). Given the potential interaction loads/time-under-tension, we evaluated hyperemic response at two different loads failure.Thirty males participated in this double-blinded study. At visit 1, participants were randomly assigned consume PWS (Reckless™) or PBO (maltodextrin and glycine) performed four...
It is currently unclear as to whether sex hormones are significantly affected by soy or whey protein consumption. Additionally, estrogenic signaling may be potentiated via supplementation due the presence of phytoestrogenic isoflavones. Limited also evidence suggests that increase androgenic signaling. Therefore, purpose this study was examine effects concentrate (SPC), (WPC), placebo (PLA) on serum hormones, androgen markers in muscle tissue, and estrogen subcutaneous (SQ) adipose tissue...