A5018614999- Fatty Acid Research and Health
- PARP inhibition in cancer therapy
- Cancer, Lipids, and Metabolism
- Adipose Tissue and Metabolism
- Infant Nutrition and Health
- Human-Automation Interaction and Safety
- Birth, Development, and Health
- Cancer, Hypoxia, and Metabolism
- Air Traffic Management and Optimization
- Radiomics and Machine Learning in Medical Imaging
- Peroxisome Proliferator-Activated Receptors
- Diet and metabolism studies
- Lipid metabolism and biosynthesis
- RNA modifications and cancer
- Breastfeeding Practices and Influences
- Teleoperation and Haptic Systems
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- Cholesterol and Lipid Metabolism
- Neonatal Respiratory Health Research
- Digestive system and related health
- Endoplasmic Reticulum Stress and Disease
- Gene expression and cancer classification
- Aerospace and Aviation Technology
- Cancer Genomics and Diagnostics
University of Oklahoma Health Sciences Center
2020-2025
University of Colorado Anschutz Medical Campus
2012-2025
OU Health
2020-2024
University of Oklahoma
2023
Deutsches Zentrum für Luft- und Raumfahrt e. V. (DLR)
2007-2022
University of Colorado Denver
2007-2020
Berlin-Brandenburger Centrum für Regenerative Therapien
2019
The Medical Center of Aurora
2018
MUSC Hollings Cancer Center
2010
Medical University of South Carolina
2010
The mammary gland of the lactating mouse synthesizes and secretes milk lipid equivalent to its entire body weight in a single 20-day lactation cycle, making it one most active synthetic organs known. We test hypothesis that multiple control points potential regulatory mechanisms regulate synthesis at level gene expression. transcriptome 130 genes involved glucose metabolism was examined late pregnancy early lactation, utilizing data obtained from microarray analysis glands quadruplicate FVB...
Tumor cells exhibit an altered metabolism characterized by elevated aerobic glycolysis and lactate secretion which is supported increase in glucose transport consumption. We hypothesized that reducing or eliminating the expression of most prominently expressed transporter(s) would decrease amount available to breast cancer thereby decreasing their metabolic capacity proliferative potential. Of 12 GLUT family transporters mice, GLUT1 was abundantly at RNA level mouse mammary tumors from...
Adipocytes undergo pronounced changes in size and behavior to support diverse tissue functions, but the mechanisms that control these are not well understood. Mammary gland-associated white adipose (mgWAT) regresses of milk fat production during lactation expands subsequent involution milk-producing epithelial cells, providing one most marked physiological examples growth. We examined cellular functional implications adipocyte lipid dynamics mouse mammary gland (MG). Using vivo analysis...
The goal of Working Group 1 in the Breastmilk Ecology: Genesis Infant Nutrition (BEGIN) Project was to outline factors influencing biological processes governing human milk secretion and evaluate our current knowledge these processes. Many regulate mammary gland development utero, during puberty, pregnancy, through secretory activation, at weaning. These include breast anatomy, vasculature, diet, lactating parent's hormonal milieu including estrogen, progesterone, placental lactogen,...
Abstract Background The molecular events underlying mammary development during pregnancy, lactation, and involution are incompletely understood. Results Mammary gland microarray data, cellular localization protein-protein interactions, literature-mined genes were integrated analyzed using statistics, principal component analysis, gene ontology pathway network analysis to identify global biological principles that govern involution. Conclusion Several key derived: (1) nearly a third of the...
The lactating mammary gland synthesizes large amounts of triglyceride from fatty acids derived the blood and de novo lipogenesis. latter is significantly increased at parturition decreased when additional dietary become available. To begin to understand molecular regulation lipogenesis, we tested hypothesis that transcription factor sterol regulatory element binding (SREBF)-1c a primary regulator this system. Expression Srebf1c mRNA six its known target genes ≥2.5-fold parturition. However,...
PAT proteins (perilipin, adipophilin, and TIP47) are hypothesized to be critical regulators of lipid accumulation in eukaryotic cells. We investigated the developmental relationships between expression these cytoplasmic droplet (CLD) differentiating secretory epithelial cells mouse mammary glands. Adipophilin (ADPH) specifically localized CLD lactating glands was found exclusively secreted fraction milk. ADPH transcripts were selectively detected cells, steady-state levels both mRNA protein...
TRC8/RNF139 encodes an endoplasmic reticulum-resident E3 ubiquitin ligase that inhibits growth in a RING- and ubiquitylation-dependent manner. TRC8 also contains predicted sterol-sensing domain. Here, we report protein levels are sterol responsive it binds stimulates ubiquitylation of the reticulum anchor INSIG. Induction destabilized precursor forms transcription factors SREBP-1 SREBP-2. Loss SREBP precursors was proteasome dependent, required functional RING domain, occurred without...
Little is known about the specific breastmilk components responsible for protective effects on infant obesity. Whether 12,13-dihydroxy-9Z-octadecenoic acid (12,13-diHOME), an oxidized linoleic metabolite and activator of brown fat metabolism, present in human milk, or linked to adiposity, unknown.To examine associations between concentrations 12,13-diHOME milk adiposity.Prospective cohort study from 2015 2019, following participants birth 6 months age.Academic medical centers.Volunteer...
Age-related endothelial dysfunction is a pivotal factor in the development of cardiovascular diseases, stemming, at least part, from mitochondrial and consequential increase oxidative stress. These alterations are central to decline vascular health seen with aging, underscoring urgent need for interventions capable restoring function preventing diseases. Dietary interventions, notably time-restricted feeding (TRF), have been identified their anti-aging effects on mitochondria, offering...
Due to the growing obesity epidemic in United States, it is now estimated that approximately 1/3 of all children are born obese moms. These data, coupled with data indicating associated accelerated cyst growth autosomal dominant polycystic kidney disease (ADPKD) patients, led us hypothesize maternal may influence rate progression offspring. To test this hypothesis, we induced by high fat diet (HFD) feeding orthologous Pkd1 RC/RC mouse model ADPKD and followed (PKD) offspring for up one year....
Abstract Background Lipid metabolic reprogramming is increasingly recognized as a hallmark of endocrine resistance in estrogen receptor-positive (ER+) breast cancer. In this study, we investigated alterations lipid metabolism ER + cancer cell lines with acquired to common therapies and evaluated the efficacy clinically relevant fatty acid synthase (FASN) inhibitor. Methods resistant Tamoxifen (TamR), Fulvestrant (FulvR), long-term withdrawal (EWD) were derived. Global gene expression...
Human milk oligosaccharides (HMOs) are integral to infant health. Yet, their complex biosynthesis pathways in the mammary gland during lactation remain under characterized. To address this knowledge gap, we performed integrated analyses of single-cell RNA-sequencing (scRNA-seq) datasets combined with select HMO concentration measures. We identify differential expression patterns known synthesis genes epithelial subsets and nominate several candidate that vary concentration. Additionally,...