A5060877008- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Body Contouring and Surgery
- Mesenchymal stem cell research
- Hair Growth and Disorders
- Wound Healing and Treatments
- Arsenic contamination and mitigation
- CRISPR and Genetic Engineering
- Skin Protection and Aging
- Muscle Physiology and Disorders
- Gene Regulatory Network Analysis
- Pluripotent Stem Cells Research
- Skin and Cellular Biology Research
- Cell Image Analysis Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Prostate Cancer Treatment and Research
- Advanced biosensing and bioanalysis techniques
- Silk-based biomaterials and applications
- Developmental Biology and Gene Regulation
- International Labor and Employment Law
- Insect Resistance and Genetics
- Labor Movements and Unions
- Heavy Metal Exposure and Toxicity
Washington University in St. Louis
2018-2023
Yale University
2014-2020
Yorkshire Cancer Research
2011-2012
University of York
2011-2012
Myofibroblast diversity with injury and aging Fibroblasts deposit extracellular matrix (ECM) molecules to regulate tissue strength function. However, if too much ECM is deposited, fibrosis scarring results. Shook et al. examined cells during mouse skin wound healing, fibrosis, (see the Perspective by Willenborg Eming). They identified distinct subpopulations of myofibroblasts, including as adipocyte precursors (APs). In cellular ablation models, CD301b-expressing macrophages selectively...
High-throughput single-cell assays increasingly require special consideration in experimental design, sample multiplexing, batch effect removal, and data interpretation. Here, we describe a lentiviral barcode-based multiplexing approach, CellTag Indexing, which uses predefined genetic barcodes that are heritable, enabling cell populations to be tagged, pooled, tracked over time the same replicate. We demonstrate utility of Indexing by sequencing transcriptomes using variety types, including...
Complex gene regulatory mechanisms underlie differentiation and reprogramming. Contemporary single-cell lineage-tracing (scLT) methods use expressed, heritable DNA barcodes to combine cell lineage readout with transcriptomics. However, reliance on transcriptional profiling limits adaptation other assays. With CellTag-multi, we present an approach that enables direct capture of random expressed as polyadenylated transcripts, in both RNA sequencing Assay for Transposase Accessible Chromatin...
In the human prostate, expression of prostate-specific genes is known to be directly regulated by androgen-induced stimulation androgen receptor (AR). However, less about control prostate-restricted TGM4 (hTGP) gene. present study we demonstrate that regulation hTGP gene depends mainly on retinoic acid (RA). We provide evidence gamma (RAR-G) plays a major role in and presence AR, but not its transcriptional transactivation activity, critical for transcription. RA responsive elements (RARE...
Abstract Complex gene regulatory mechanisms underlie differentiation and reprogramming. Contemporary single-cell lineage tracing (scLT) methods use expressed, heritable DNA barcodes to combine cell readout with transcriptomics enabling high-resolution analysis of states while preserving relationships. However, reliance on transcriptional profiling limits their adaptation an ever-expanding tool kit multiomic assays. With CellTag-multi, we present a novel approach for independently chromatin...
ABSTRACT Single-cell technologies have seen rapid advancements in recent years, presenting new analytical challenges and opportunities. These high-throughput assays increasingly require special consideration experimental design, sample multiplexing, batch effect removal, data interpretation. Here, we describe a lentiviral barcode-based multiplexing approach, ‘CellTag Indexing’, where transduce label samples that can then be pooled together for downstream experimentation analysis. By...
White adipose tissue is crucial in various physiological processes. In response to high caloric intake, may expand by generating new adipocytes. Adipocyte precursor cells (progenitors and preadipocytes) are essential for mature adipocytes, single-cell RNA sequencing provides means identify these populations. Here, we characterized adipocyte populations the skin, an depot with rapid robust generation of We identified a population immature preadipocytes, revealed biased differentiation...