A5033962742- Estrogen and related hormone effects
- Dermatology and Skin Diseases
- Vitamin D Research Studies
- Skin Protection and Aging
- Cytokine Signaling Pathways and Interactions
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Hair Growth and Disorders
- Digestive system and related health
- T-cell and B-cell Immunology
- IL-33, ST2, and ILC Pathways
- Cancer, Lipids, and Metabolism
- Intestinal and Peritoneal Adhesions
- Immunotherapy and Immune Responses
- Lung Cancer Research Studies
- Cholesterol and Lipid Metabolism
- Microbial infections and disease research
- Vitamin C and Antioxidants Research
- Chemokine receptors and signaling
- Asthma and respiratory diseases
- Protein purification and stability
- Legionella and Acanthamoeba research
- Barrier Structure and Function Studies
Wroclaw Medical University
2024
Northwestern University
2015-2023
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2015-2019
Polish Academy of Sciences
2015-2019
Karolinska Institutet
2019
University of Wrocław
2011-2016
Purdue University West Lafayette
2004-2008
University of Chicago
2007
We have shown that 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) down-regulates renin expression. To explore the molecular mechanism, we analyzed mouse Ren-1c gene promoter by luciferase reporter assays. Deletion analysis revealed two DNA fragments from -2,725 to -2,647 (distal fragment) and -117 +6 (proximal are sufficient mediate repression. Mutation of cAMP response element (CRE) in distal fragment blunted forskolin stimulation as well 1,25(OH)(2)D(3) inhibition transcriptional activity,...
Abstract Emerging bacterial antibiotic resistance draws attention to bacteriophages as a therapeutic alternative treat infection. Examples of phage that combat bacteria abound. However, despite careful testing antibacterial activity in vitro , failures nevertheless commonly occur. We investigated immunological response potency vivo . Anti-phage phagocytes, antibodies and serum complement were identified by direct high-resolution fluorescent microscopy. accommodated the experimental data into...
A specific humoral response to bacteriophages may follow phage application for medical purposes, and it further determine the success or failure of approach itself. We present a long-term study antibody induction in mice by T4 applied per os: 100 days treatment followed 112 without phage, subsequent second up day 240. Serum gut antibodies (IgM, IgG, secretory IgA) were analyzed relation microbiological status animals. orally induced anti-phage when exposure was long enough (IgG 36, IgA 79);...
Bacteriophages that infect Gram-negative bacteria often bind to the bacterial surface by interaction of specific proteins with lipopolysaccharide (LPS). Short tail fiber (tail adhesin, gp12) mediate adsorption T4-like bacteriophages Escherichia coli, binding or LPS. Produced as a recombinant protein, gp12 retains its ability Since LPS is able exert major impact on immune response in animals and humans, we have tested LPS-binding phage protein potential modulator LPS-induced response. We...
Summary Bacteriophages draw scientific attention in medicine and biotechnology, including phage engineering, widely used to shape biological properties of bacteriophages. We developed engineered T4‐derived bacteriophages presenting seven types tissue‐homing peptides. evaluated accumulation targeted tissues, spleen, liver circulation blood (in mice). Contrary expectations, organs was not observed, but instead, three phages achieved tissue titres up 2 orders magnitude lower than unmodified T4....
Calbindin D 9k (CaBP) is critical for intestinal calcium absorption; its in vivo expression restricted to differentiated enterocytes of the small intestine. Our goal was identify factors controlling transcriptional regulation this gene human Both natural and a 4600-bp promoter construct were strongly regulated by differentiation (>100-fold) but not treatment with 1,25(OH) 2 vitamin (<2-fold) Caco-2 clone TC7. Deletion-mutation studies revealed that conserved sequences cdx-2 (at −3158...
Glucocorticoids are effective anti-inflammatory drugs widely used in dermatology and for the treatment of blood cancer patients. Unfortunately, chronic with glucocorticoids results serious metabolic atrophogenic adverse effects including skin atrophy. act via glucocorticoid receptor (GR), a transcription factor that causes either gene transactivation (TA) or transrepression (TR). Compound A (CpdA), novel non-steroidal GR ligand, does not promote dimerization TA, retains potential but induces...
Abstract We examined the effects of 1,25 dihydroxyvitamin D 3 (1,25(OH) 2 ) on distribution and mobility vitamin receptor (VDR) in enterocyte‐like Caco‐2 cell. Confocal microscopy showed that a green fluorescent protein‐vitamin (GFP‐VDR) fusion protein is predominantly nuclear (58%) it does not associate with apical or basolateral membrane proliferating polarized, differentiated cells. In contrast to previously studied cell types, neither endogenous VDR nor GFP‐VDR levels accumulate nucleus...
The most common method for phage quantitation is the plaque assay,which relies on ability to infect bacteria. However, non-infective particles may preserve other biological properties; specifically, they enter interactions with immune system of animals and humans. Here we demonstrate real-time quantitative polymerase chain reaction (qPCR) detection bacteriophages as an alternative assay. closely related staphylococcal A3R 676Z coliphage T4 were used model phages. They tested in vivo mice, ex...
Abstract We examined 1,25 dihydroxyvitamin D (1,25(OH) 2 3 )‐induced expression of 25‐hydroxyvitamin 24‐hydroxylase (CYP24) and apical calcium channel (TRPV6) mRNA levels in 2‐, 9‐, 15‐day cultures Caco‐2 cells that model proliferating, post‐proliferative, differentiated enterocytes. 1,25(OH) ‐induced (10 nM, 8 h) CYP24 TRPV6 were significantly greater post‐proliferative than proliferating (>16X >3X, respectively). Neither half‐life nor induction a −298 bp rat promoter‐luciferase...
Glucocorticoids are widely used for therapy of hematologic malignancies. Unfortunately, chronic treatment with glucocorticoids commonly leads to adverse effects including skin and muscle atrophy osteoporosis. We found recently that REDD1 (regulated in development DNA damage 1) plays central role steroid atrophy. Here, we tested whether suppression makes glucocorticoid-based blood cancer safer. Unexpectedly, approximately 50% top putative inhibitors selected by bioinformatics screening...
Better understanding of molecular changes leading to neoplastic transformation is prerequisite optimize risk assessment and chemopreventive surveillance strategies. Data on macrophage inflammatory proteins (MIPs) in colorectal carcinogenesis are scanty their clinical relevance remains unknown. Therefore, transcript protein expression CCL3, CCL4, CXCL2, CCL19 were determined 173 62 patients, respectively, using RT-qPCR immunohistochemistry with reference polyps' characteristics. The...
Differences in prevalence of inflammatory skin diseases including atopic dermatitis and psoriasis African American (AA) versus White Non-Hispanic (WNH) population are well recognized. However, the underlying mechanisms largely unknown. We previously observed significant differences healthy AA transcriptome with differentially expressed genes (DEG) enriched for inflammation cornification processes. Here we analyzed proteome biopsies from WNH volunteers using Olink® Explore Inflammation 384...
<div>Abstract<p>Glucocorticoids are widely used for therapy of hematologic malignancies. Unfortunately, chronic treatment with glucocorticoids commonly leads to adverse effects including skin and muscle atrophy osteoporosis. We found recently that REDD1 (regulated in development DNA damage 1) plays central role steroid atrophy. Here, we tested whether suppression makes glucocorticoid-based blood cancer safer. Unexpectedly, approximately 50% top putative inhibitors selected by...
<div>Abstract<p>Glucocorticoids are widely used for therapy of hematologic malignancies. Unfortunately, chronic treatment with glucocorticoids commonly leads to adverse effects including skin and muscle atrophy osteoporosis. We found recently that REDD1 (regulated in development DNA damage 1) plays central role steroid atrophy. Here, we tested whether suppression makes glucocorticoid-based blood cancer safer. Unexpectedly, approximately 50% top putative inhibitors selected by...
<p>Supplementary Figures 1-9. Suppl. Fig. 1 contains quantification of Western blots. 2 demonstrates the effect PI3K/mTOR/Akt inhibitors on regulation endogenous genes by Dex in CEM and Granta cells. 3 shows cytotoxic effects LY294002, Wortmannin AZD8055 4 cells, cells normal human monocytes. 5 anti-lymphoma Rapamycin 6 animal body weight xenograft study. 7 anti-tumor Dex, Rapa, LY294002 xenografts. 8 Dexamethasone-induced osteoporosis 9 data Q-PCR analysis Col1a1 Col2a1 mRNA...