A5022599574- Vitamin D Research Studies
- Retinoids in leukemia and cellular processes
- Acute Myeloid Leukemia Research
- Estrogen and related hormone effects
- Reproductive System and Pregnancy
- Eosinophilic Disorders and Syndromes
- Fibroblast Growth Factor Research
- Immune Cell Function and Interaction
- Histone Deacetylase Inhibitors Research
- Biotin and Related Studies
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Kruppel-like factors research
- Immunodeficiency and Autoimmune Disorders
- Virus-based gene therapy research
University of Wrocław
2012-2024
All-trans-retinoic acid (ATRA) and 1α,25-dihydroxyvitamin D (1,25D) are potent inducers of differentiation myeloid leukemia cells. During specific transcription factors expressed at crucial developmental stages. However, precise mechanism controlling the diversification progenitors is largely unknown, CCAAT/enhancer-binding protein (C/EBP) have been characterized as key regulators development function system. Past data point functional redundancy among C/EBP family members during...
Acute myeloid leukemia (AML) is the predominant acute among adults, characterized by an accumulation of malignant immature precursors. A very promising way to treat AML differentiation therapy using either all-trans-retinoic acid (ATRA) or 1,25-dihydroxyvitamin D3 (1,25D), use both these differentiation-inducing agents. However, effect combination treatment varies in different cell lines, and this due ATRA down- up-regulating transcription vitamin D receptor (VDR) cells examined. The...
Vitamin D receptor (VDR) is present in multiple blood cells, and the hormonal form of vitamin D, 1,25-dihydroxyvitamin (1,25D) essential for proper functioning immune system. The role retinoic acid α (RARα) hematopoiesis very important, as fusion RARα gene with PML initiates acute promyelocytic leukemia where differentiation myeloid lineage blocked, followed by an uncontrolled proliferation leukemic blasts. takes part regulation VDR transcription, unliganded acts a transcriptional repressor...
Activities of the retinoic acid receptor (RAR)α and RARγ are important to hematopoiesis. Here, we have investigated effects selective agonists antagonists on primitive human hematopoietic cell lines KG1 NB-4 purified normal stem cells (HSCs). Agonizing RARα (by AGN195183) was effective in driving neutrophil differentiation this agonist synergized with a low amount (10 nM) 1α,25-dihydroxyvitamin D3 drive monocyte cells. Treatment cultures HSCs (supplemented factor ± interleukin 3) an...
1α,25-Dihydroxyvitamin D(3) (1,25(OH)(2)D) exerts its biological activities through vitamin D receptor (VDR), which is a member of the superfamily steroid receptors, that act as ligand-dependent transcription factors. Ligated VDR in complex with retinoid X (RXR) binds to regulatory regions 1,25(OH)(2)D-target genes. 1,25(OH)(2)D able induce differentiation leukemic blasts towards macrophage-like cells. Many different acute myeloid leukemia (AML) cell lines respond by increasing CD14 surface...
Acute myeloid leukemia (AML) cell lines can be driven to differentiate monocyte-like cells by 1,25- dihydroxyvitamin D3 (1,25D) and granulocyte-like all-trans-retinoic acid (ATRA). Both compounds activate their specific intracellular receptors, vitamin D receptor (VDR) retinoic receptors (RARs) respectively. Inside the 1,25D is degraded calcitrioic a mitochondrial enzyme CYP24A1, while ATRA several polar metabolites CYP26. NADPH-cytochrome P450 oxidoreductase (POR) membrane-bound required...
Acute myeloid leukemia (AML) cells can be induced to undergo terminal differentiation with subsequent loss of tumorigenicity using 1,25-dihydroxyvitamin D3 (1,25D) alone or in combination hematopoietic cytokines. KG1 are resistant 1,25D-induced cell differentiation. These have the aberrant signal transduction resulting from a constitutively active fusion protein FOP2-FGFR1, STAT1 and high level interferon (IFN) stimulated genes (ISGs). In this paper we report that activated FOP2-FGFR1...
(1) Hematological malignancies are characterized by an immortalization, uncontrolled proliferation of blood cells and their differentiation block, followed the loss function. The primary goal in treatment leukemias is elimination rapidly proliferating leukemic (named blasts). However, chemotherapy, which removes blasts, also prevents remaining immune from being activated. Acute affect elderly people, who often not fit to survive aggressive chemotherapy. Therefore, there a need milder...
(1) Background: Vitamin D receptor (VDR) is present in multiple types of blood cells, and its ligand, 1,25-dihydroxyvitamin (1,25D), important for the proper functioning immune system. Activity VDR higher hematopoietic stem progenitor cells than fully differentiated mice humans. In some human acute myeloid leukemia (AML) blasts, expression gene also high. The mechanism silencing during differentiation has been addressed this work. (2) Methods: have obtained using fluorescence activated...
(1) Background: Many malignancies are driven by mutations which affect the gene for fibroblast growth factor receptor (FGFR) 1. Previously we have documented that signal transduction from FOP2–FGFR1 fusion protein in KG1 cells downregulated expression of vitamin D (VDR) gene. In this paper investigated if also other FGFRs were responsible regulation VDR expression. (2) Methods: We used human myeloid leukemia U937, and bone cancer cell line U2OS, transfection methods order to...