A5058215374- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Microbial infections and disease research
- Prostate Cancer Diagnosis and Treatment
- Plant and animal studies
- Protein purification and stability
- Health Systems, Economic Evaluations, Quality of Life
- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- Streptococcal Infections and Treatments
- Global Cancer Incidence and Screening
- Colorectal Cancer Screening and Detection
- Genomics and Phylogenetic Studies
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
Lublin Oncology Center
2024
Pure Biologics (Poland)
2020-2021
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2016-2020
Polish Academy of Sciences
2015-2020
Abstract Emerging bacterial antibiotic resistance draws attention to bacteriophages as a therapeutic alternative treat infection. Examples of phage that combat bacteria abound. However, despite careful testing antibacterial activity in vitro , failures nevertheless commonly occur. We investigated immunological response potency vivo . Anti-phage phagocytes, antibodies and serum complement were identified by direct high-resolution fluorescent microscopy. accommodated the experimental data into...
A specific humoral response to bacteriophages may follow phage application for medical purposes, and it further determine the success or failure of approach itself. We present a long-term study antibody induction in mice by T4 applied per os: 100 days treatment followed 112 without phage, subsequent second up day 240. Serum gut antibodies (IgM, IgG, secretory IgA) were analyzed relation microbiological status animals. orally induced anti-phage when exposure was long enough (IgG 36, IgA 79);...
Bacteriophages that infect Gram-negative bacteria often bind to the bacterial surface by interaction of specific proteins with lipopolysaccharide (LPS). Short tail fiber (tail adhesin, gp12) mediate adsorption T4-like bacteriophages Escherichia coli, binding or LPS. Produced as a recombinant protein, gp12 retains its ability Since LPS is able exert major impact on immune response in animals and humans, we have tested LPS-binding phage protein potential modulator LPS-induced response. We...
While the true efficacy of phage therapy still requires formal confirmation in clinical trials, it continues to offer realistic potential treatment patients whom antibiotics have failed. Novel developments and approaches are therefore needed ascertain that future trials would evaluate its optimal form thus allowing for reliable conclusions regarding value therapy. In this article, we present our vision develop establish a bank phages specific most threatening pathogens armed with homing...
Summary Bacteriophages draw scientific attention in medicine and biotechnology, including phage engineering, widely used to shape biological properties of bacteriophages. We developed engineered T4‐derived bacteriophages presenting seven types tissue‐homing peptides. evaluated accumulation targeted tissues, spleen, liver circulation blood (in mice). Contrary expectations, organs was not observed, but instead, three phages achieved tissue titres up 2 orders magnitude lower than unmodified T4....
Background: Bacteriophages may induce specific antibodies after natural exposure to phages or phage therapy. As such, phage-specific might impact bioavailability in vivo, although limited non-neutralizing insignificant effects have also been reported. Materials and Methods: Here, we report antibody induction against PB1-related (Pseudomonas viruses LMA2, F8, DP1) mice over an 80-day period, for a healthy population of humans, patients undergoing therapy (oral and/or topical treatment)....
The aim of this study was the isolation and characterization, including phage effect on honeybees in laboratory conditions, phages active against
Only 3% of phage genomes in NCBI nucleotide database represent phages that are active against Streptococcus sp. With the aim to increase general awareness diversity, we isolated two bacteriophages, Str01 and Str03, health-threatening Group A (GAS). Both members Siphoviridae, but their analysis revealed Str03 do not belong any known genus. We identified structural proteins based on LC-ESI29 MS/MS list basic thermal stability physico-chemical features including optimum pH. Annotated genomic...