The interest for natural antimicrobial compounds has increased due to alterations in consumer positions towards the use of chemical preservatives foodstuff and food processing surfaces. Bacteriophages fit class their effectiveness controlling bacterial pathogens agro-food industry led development different phage products already approved by USFDA USDA. majority these are be used farm animals or animal such as carcasses, meats also agricultural horticultural products. Treatment with specific...

Resistance rates are increasing among several problematic Gram-negative pathogens, a fact that has encouraged the development of new antimicrobial agents. This paper characterizes Salmonella phage endolysin (Lys68) and demonstrates its potential effectiveness when combined with organic acids towards pathogens. Biochemical characterization reveals Lys68 is more active at pH 7.0, maintaining 76.7% activity stored 4°C for two months. Thermostability tests showed only completely inactivated upon...

The present study demonstrates the antibacterial potential of a phage endolysin against Gram-negative pathogens, particularly multidrug resistant strains Acinetobacter baumannii. We have cloned, heterologously expressed and characterized novel (ABgp46) from vb_AbaP_CEB1 tested its activity several multidrug-resistant A. baumannii strains. LC-MS revealed that ABgp46 is an N-acetylmuramidase, also active over broad pH range (4.0-10.0) temperatures up to 50°C. Interestingly, has intrinsic...

Proteus mirabilis is an enterobacterium that causes catheter-associated urinary tract infections (CAUTIs) due to its ability colonize and form crystalline biofilms on the catheters surface. CAUTIs are very difficult treat, since biofilm structures highly tolerant antibiotics. Phages have been used widely control a diversity of bacterial species, however limited number phages for P. isolated studied. Here we report isolation two novel virulent phages, podovirus vB_PmiP_5460 myovirus...

Pseudomonas fluorescens is an important food spoilage organism, usually found in the form of biofilms. Bacterial biofilms are inherently resistant to a variety antimicrobial agents, therefore alternative methods biofilm control, such as bacteriophages (phages) have been suggested. Phage behavior on still poorly investigated and needs further understanding. Here we describe application phage phiIBB-PF7, newly isolated phage, control P. The were formed under static or dynamic conditions with...

Abstract Background Despite the proven relevance of Pseudomonas fluorescens as a spoilage microorganism in milk, fresh meats and refrigerated food products recognized potential bacteriophages sanitation agents, so far no phages specific for P. isolates from dairy industry have been closely characterized view their lytic efficiency. Here we describe isolation characterization phage capable to infect variety strains isolated Portuguese United States industries. Results Several were which...

Abstract Background The Double-Layer Agar (DLA) technique is extensively used in phage research to enumerate and identify phages isolate mutants new phages. Many form large well-defined plaques that are easily observed so they can be enumerated when plated by the DLA technique. However, some give rise small turbid very difficult detect count. To overcome these problems, authors have suggested use of dyes improve contrast between host lawns. It has been reported antibiotics stimulate bacteria...

Abstract This study investigated the phenotypic characteristics of monoculture P. fluorescens biofilms grown under turbulent and laminar flow, using flow cells reactors with stainless steel substrata. The cellular physiology overall biofilm activity, structure composition were characterized, compared, within hydrodynamically distinct conditions. results indicate that flow-generated significantly less extensive, decreased metabolic activity a lower protein polysaccharides per cell than those...

Despite the recent enthusiasm for using bacteriophages as bacterial control agents, there are only limited studies concerning phage interaction with their respective hosts residing in mixed biofilm consortia and especially biofilms where host species is a minor constituent. In present work, study was made of mono dual formed by Pseudomonas fluorescens (Gram-negative) and/or Staphylococcus lentus (Gram-positive) fate after infection phages. The consisted predominantly S. lentus. exposure...

Summary Bacteriophages are ubiquitous in nature and represent a vast repository of genetic diversity, which is driven by the endless coevolution cycle with diversified group bacterial hosts. Studying phage‐host interactions important to gain novel insights into their dynamic adaptation. In this study, we isolated 12 phages infecting species Acinetobacter baumannii‐Acinetobacter calcoaceticus complex exhibited narrow host range similar morphological features (podoviruses short tails 9–12 nm...

Chronic wounds afford a hostile environment of damaged tissues that allow bacterial proliferation and further wound colonization.

Chronic skin wounds represent a major burn both economically and socially. Pseudomonas aeruginosa Escherichia coli are among the most common colonizers of infected prolific biofilm formers. Biofilms problem in infections due to their increasingly difficult control eradication, tolerance multiple prescribed drugs. As so, alternative methods necessary. Bacteriophages (phages) honey seen as promising approach for related infections. Phages have specificity toward bacterial genus, species or...

Staphylococcus epidermidis is considered an important nosocomial pathogen, being very tolerant to the host immune system and antibiotherapy, particularly when in biofilms. Due its high resistance, alternative antimicrobial strategies are under development. The use of bacteriophages seen as strategy combat pathogenic organisms. In this study, a S. myovirus, SEP1, was isolated characterized. genome phage sequenced shown be related peripherally genus Twortlikevirus. However, compared with other...

Implantable long-acting delivery systems able to minimize off-target side effects and locally provide non-invasive imaging reporting are of utmost importance offer a precise treatment internal diseases accurate assessment disease progression, such as in local infections tumor relapse following surgery, among other diseases. Here, biocompatible xanthan gum/Fe3O4-based drug-loaded magnetic nanoparticle composite hydrogel with suitable rheological properties theranostic performance was...

This communication focuses on the efficacy of a specific lytic phage, phage F S1, as control agent Pseudomonas fluorescens biofilms. The effect infection temperature and host growth were evaluated. results obtained showed that process was dependent optimum planktonic cells biofilms 26°C. At this temperature, bacteriophage at multiplicity (MOI) 0.5 infected both causing biomass reduction about 85% in cases.