A5078264604- Electrospun Nanofibers in Biomedical Applications
- Wound Healing and Treatments
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- 3D Printing in Biomedical Research
- Silk-based biomaterials and applications
- Diabetic Foot Ulcer Assessment and Management
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Pluripotent Stem Cells Research
- Pressure Ulcer Prevention and Management
- Connective tissue disorders research
- Spine and Intervertebral Disc Pathology
- Bone Tissue Engineering Materials
- Cellular Mechanics and Interactions
- RNA Interference and Gene Delivery
- Polymer Surface Interaction Studies
- Medical Imaging and Analysis
- Protease and Inhibitor Mechanisms
- Cleft Lip and Palate Research
- Silkworms and Sericulture Research
- Spinal Cord Injury Research
- Periodontal Regeneration and Treatments
- Peripheral Artery Disease Management
- Musculoskeletal pain and rehabilitation
Yale University
2014-2023
Creative Commons
2020-2022
University of New Haven
2021
Quinnipiac University
2020
Plastic Surgery Hospital
2019
Yale Cancer Center
2015-2016
Indian Institute of Technology Kharagpur
2008-2012
National University of Ireland
2012
Ollscoil na Gaillimhe – University of Galway
2010-2011
Myofibroblast diversity with injury and aging Fibroblasts deposit extracellular matrix (ECM) molecules to regulate tissue strength function. However, if too much ECM is deposited, fibrosis scarring results. Shook et al. examined cells during mouse skin wound healing, fibrosis, (see the Perspective by Willenborg Eming). They identified distinct subpopulations of myofibroblasts, including as adipocyte precursors (APs). In cellular ablation models, CD301b-expressing macrophages selectively...
Abstract Diabetic foot ulceration (DFU) is a devastating complication of diabetes whose pathogenesis remains incompletely understood. Here, we profile 174,962 single cells from the foot, forearm, and peripheral blood mononuclear using single-cell RNA sequencing. Our analysis shows enrichment unique population fibroblasts overexpressing MMP1, MMP3, MMP11, HIF1A, CHI3L1 , TNFAIP6 increased M1 macrophage polarization in DFU patients with healing wounds. Further, spatially separated samples same...
There is an urgent need for efficient approach to obtain a large-scale and renewable source of functional human vascular smooth muscle cells (VSMCs) establish robust, patient-specific tissue model systems studying the pathogenesis disease, developing novel therapeutic interventions. Here, we have derived large quantity highly enriched VSMCs from induced pluripotent stem (hiPSC-VSMCs). Furthermore, engineered 3D rings hiPSC-VSMCs using facile one-step cellular self-assembly approach. The are...
Aim: To assess the potential of human induced pluripotent stem cell-derived smooth muscle cells (hiPSC-SMC) to accelerate diabetic wound healing. Methods: hiPSC-SMC were embedded in 3D collagen scaffolds and cultured vitro for 72 h; then applied diabetic, nude mouse, splinted back wounds vivo Cultured medium after scaffold incubation was collected analyzed expression pro-angiogenic cytokines. Results: secrete increased concentration cytokines, compared with murine adipose derived cells....
Nonviral gene delivery systems have a number of limitations including low transfection efficiency, specificity, and cytotoxicity, especially when the target cells are macrophages. To address these issues, hypothesis tested in this study was that mannose functionalized nanohybrids composed synthetic natural polymers will improve cell viability, specificity Robust were designed from hyaluronic acid (HA) branched polyethyleneimine (bPEI) using carbodiimide chemistry. The reaction product, i.e.,...
The application of human-induced pluripotent stem cells (hiPSCs) to generate vascular smooth muscle (hiPSC-VSMCs) in abundance is a promising strategy for regeneration. While hiPSC-VSMCs have already been utilized tissue-engineered grafts and disease modeling, there lack investigations exploring their therapeutic secretory factors. objective this manuscript was understand how the biophysical property collagen-based scaffold dictates changes function while developing hiPSC-VSMC-based therapy...
A commonly used treatment for open wounds, negative pressure wound therapy (NPWT) has recently been to optimize healing in the setting of surgically closed wounds; however, specific mechanisms action by which NPWT may benefit patients after surgery remain unknown. Using a swine model, current study investigates angiogenesis as candidate mechanism.Multiple excisional wounds were created on dorsa 10 male Yorkshire pigs and primary suture. The underwent with either dressing or control dressings...
Human-induced pluripotent stem cell-derived vascular smooth muscle cells (hiPSC-VSMCs) with proangiogenic properties have huge therapeutic potential. While hiPSC-VSMCs already been utilized for wound healing using a biomimetic collagen scaffold, an in situ forming hydrogel mimicking the native environment of skin offers promise hiPSC-VSMC mediated repair and regeneration. Herein, impact type-I-hyaluronic acid (HA) cross-linked polyethylene glycol-based cross-linker on viability paracrine...
Disorders related to the intervertebral disc (IVD) are common causes of morbidity and severe life quality deterioration. IVD degeneration, although in many cases asymptomatic, is often origin o
Encapsulation of plasmid DNA (pDNA) in nanoparticulate gene delivery systems offers the possibility control dosing, enhanced pDNA uptake, increased resistance to nuclease degradation and sustained release functionally active over time. Extracellular matrix based biomaterial i.e. hyaluronan (HA) was used encapsulate (pCMV-GLuc, Gaussia Luciferase reporter having CMV promoter) submicron size particulate system. Nano range (~400-600 nm) loaded nanoparticles were formulated by ionic gelation...
Wound repair requires the coordination of multiple cell types including immune cells and tissue resident to coordinate healing return function. Diabetic foot ulceration is a type chronic wound that impacts over 4 million patients in US 7 worldwide (Edmonds et al., 2021). Yet, cellular molecular mechanisms go awry these wounds are not fully understood. Here, by profiling ulcers from non-diabetic (NDFUs) diabetic (DFUs) using single-cell RNA sequencing, we find DFUs display transcription...
Tissue-engineered constructs have immense potential as autologous grafts for wound healing. Despite the rapid advancement in fabrication technology, major limitation is controlling angiogenesis within these to form a vascular network. Here, we aimed develop 3D hydrogel that can regulate angiogenesis. We tested effect of fibronectin and smooth muscle cells derived from human induced pluripotent stem (hiPSC-VSMC) on morphogenesis endothelial cells. The results demonstrate increases number EC...