A5044204492- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Exercise and Physiological Responses
- Lipid metabolism and biosynthesis
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Muscle metabolism and nutrition
- Cardiovascular Disease and Adiposity
- Thermoregulation and physiological responses
- Circadian rhythm and melatonin
- Muscle Physiology and Disorders
- Metabolomics and Mass Spectrometry Studies
- Fatty Acid Research and Health
- Peroxisome Proliferator-Activated Receptors
- Alkaline Phosphatase Research Studies
- Metabolism, Diabetes, and Cancer
- Mesenchymal stem cell research
- Sirtuins and Resveratrol in Medicine
- Tissue Engineering and Regenerative Medicine
- Telomeres, Telomerase, and Senescence
- Cancer, Hypoxia, and Metabolism
- Mass Spectrometry Techniques and Applications
- Eicosanoids and Hypertension Pharmacology
- Biotin and Related Studies
- Digestive system and related health
MaineHealth
2023-2025
Northeastern University
2023-2025
University of Maine
2023-2025
Harvard University
2014-2023
Maine Medical Center
2022-2023
Maine Medical Center Research Institute
2022-2023
Joslin Diabetes Center
2012-2022
Harvard Stem Cell Institute
2015-2022
Brigham and Women's Hospital
2018
Boston University
2010-2011
Abstract Objective To determine whether biopsy specimens obtained from systemic sclerosis (SSc) lesions show a distinctive gene profile, that profile is maintained in fibroblasts cultured SSc skin specimens, and results tissue multiple clinical centers can be combined to yield useful observations this rare disease. Methods Biopsy samples passaged were stored RNAlater solution prior processing for RNA. RNA control as well explanted passage 4 fibroblasts, 9 patients controls was hybridized...
Activation of energy expenditure in thermogenic fat is a promising strategy to improve metabolic health, yet the dynamic processes that evoke this response are poorly understood. Here we show synthesis mitochondrial phospholipid cardiolipin indispensable for stimulating and sustaining function. Cardiolipin biosynthesis robustly induced brown beige adipose upon cold exposure. Mimicking through overexpression synthase (Crls1) enhances consumption mouse human adipocytes. Crls1 deficiency...
Human white adipocytes engineered to express UCP1 activate endogenous BAT and protect against diet-induced obesity when transplanted into mice.
Uncoupling protein-1 (UCP1) plays a central role in energy dissipation brown adipose tissue (BAT). Using high-throughput library screening of secreted peptides, we identify two fibroblast growth factors (FGF), FGF6 and FGF9, as potent inducers UCP1 expression adipocytes preadipocytes. Surprisingly, this occurs through mechanism independent adipogenesis involves FGF receptor-3 (FGFR3), prostaglandin-E2 interaction between estrogen receptor-related alpha, flightless-1 (FLII)...
Brown adipose tissue dissipates chemical energy in the form of heat and regulates triglyceride glucose metabolism body. Factors that regulate fatty acid uptake oxidation brown adipocytes have not yet been fully elucidated. Bone morphogenetic protein 7 (BMP7) is a growth factor capable inducing fat mitochondrial biogenesis during differentiation from adipocyte progenitors. Administration BMP7 to mice also results increased expenditure. To determine if able affect activity mature adipocytes,...
Fluorescence-activated cell sorting (FACS) strategies to purify distinct types from the pool of fetal human myofiber-associated (hMFA) cells were developed. We demonstrate that expressing satellite marker PAX7 are highly enriched within subset CD45(-)CD11b(-)GlyA(-)CD31(-)CD34(-)CD56(int)ITGA7(hi) hMFA cells. These lack adipogenic capacity but exhibit robust, bipotent myogenic and osteogenic activity in vitro engraft myofibers when transplanted into mouse muscle. In contrast,...
Highlights•We report lipid profiles of cold-exposed humans and mice by MS/MSALL shotgun lipidomics•We profiled circulating as well brown white adipose lipids in response to cold•We identified a bio-signature cardiolipin biogenesis cold mice•We created an online tool visualize changes specific species our studySummaryThermogenic fat expends energy during for temperature homeostasis, its activity regulates nutrient metabolism insulin sensitivity. We measured cold-activated landscapes...
Thermoneutral conditions typical for standard human living environments result in brown adipose tissue (BAT) involution, characterized by decreased mitochondrial mass and increased lipid deposition. Low BAT activity is associated with poor metabolic health, reactivation may confer therapeutic potential. However, the molecular drivers of this adaptive process response to thermoneutrality remain enigmatic. Using lipidomic approaches, we show that endogenous fatty acid synthesis, regulated...
Insulin resistance (IR) can increase atherosclerotic and cardiovascular risk by inducing endothelial dysfunction, decreasing nitric oxide (NO) production, accelerating arterial inflammation. The aim is to determine the mechanism which insulin action NO production in cells improve systemic bioenergetics decrease atherosclerosis via differentiation of perivascular progenitor (PPCs) into brown adipocytes (BAT).Studies used various transgenic deletion mutant ApoE-/- mice receptors, eNOS...
Abstract Noradrenaline (NA) regulates cold-stimulated adipocyte thermogenesis 1 . Aside from cAMP signalling downstream of β-adrenergic receptor activation, how NA promotes thermogenic output is still not fully understood. Here, we show that coordinated α -adrenergic (AR) and β 3 -AR induces the expression genes futile creatine cycle 2,3 , early B cell factors, oestrogen-related receptors PGC1α are required for this response in vivo. triggers physical functional coupling between subtype...
The mechanisms of the saturable component long-chain fatty acid (LCFA) transport across small intestinal epithelium and its regulation by a high-fat diet (HFD) are uncertain. It is hypothesized here that putative translocase/CD36 alkaline phosphatases (IAPs) function together to optimize LCFA transport. Phosphorylated CD36 (pCD36) was expressed in mouse enterocytes dephosphorylated calf IAP (CIAP). Uptake fluorescently tagged into isolated enteroctyes increased when cells were treated with...
Opsin3 (Opn3) is a transmembrane heptahelical G protein-coupled receptor (GPCR) with the potential to produce nonvisual photoreceptive effect. Interestingly, anatomical profiling of GPCRs reveals that Opn3 mRNA highly expressed in adipose tissue. The photosensitive functions mammals are poorly understood, and whether has role fat entirely unknown. In this study, we found Opn3-knockout (Opn3-KO) mice were prone diet-induced obesity insulin resistance. At cellular level, Opn3-KO brown...
Little is known about the specific breastmilk components responsible for protective effects on infant obesity. Whether 12,13-dihydroxy-9Z-octadecenoic acid (12,13-diHOME), an oxidized linoleic metabolite and activator of brown fat metabolism, present in human milk, or linked to adiposity, unknown.To examine associations between concentrations 12,13-diHOME milk adiposity.Prospective cohort study from 2015 2019, following participants birth 6 months age.Academic medical centers.Volunteer...
Activating brown adipose tissue (BAT) improves systemic metabolism, making it a promising target for metabolic syndrome. BAT is activated by 12,13-dihydroxy-9Z-octadecenoic acid (12,13-diHOME), which we previously identified to be inversely associated with BMI and directly metabolism in multiple tissues. Here profile plasma lipidomics from 83 people test lipids' association replicates concordant direction using our novel tool ScreenDMT, whose power validity demonstrate via mathematical...