A5036287436- Metabolomics and Mass Spectrometry Studies
- Liver Disease Diagnosis and Treatment
- Drug-Induced Hepatotoxicity and Protection
- Pharmacogenetics and Drug Metabolism
- Advanced Chemical Sensor Technologies
- Pharmacological Effects of Natural Compounds
- Liver Disease and Transplantation
- Organ Transplantation Techniques and Outcomes
- Infant Nutrition and Health
- Gene expression and cancer classification
- Microtubule and mitosis dynamics
- Lipid metabolism and biosynthesis
- RNA Interference and Gene Delivery
- Drug Transport and Resistance Mechanisms
- Fungal and yeast genetics research
- Traditional Chinese Medicine Studies
- Pharmacological Effects and Toxicity Studies
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Adipose Tissue and Metabolism
- Computational Drug Discovery Methods
- Advanced Proteomics Techniques and Applications
- Histone Deacetylase Inhibitors Research
- HIV-related health complications and treatments
Universitat de València
2022-2025
Instituto de Investigación Sanitaria La Fe
2022-2025
Instituto de Salud Carlos III
2022-2025
Leitat Technological Center
2020-2024
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2022-2024
Centro de Investigación Biomédica en Red
2024
University of Southern Denmark
2018-2020
University of Fribourg
2015-2017
Instituto de Biología Molecular y Celular de Plantas
2011-2012
Hospital de Sant Pau
1991
Learning to let go with age: Intracellular controlled release of molecules within senescent cells was achieved using mesoporous silica nanoparticles (MSNs) capped a galacto-oligosaccharide (GOS) contain the cargo (magenta spheres; see scheme). The GOS is substrate biomarker, senescence-associated β-galactosidase (SA-β-gal), and releases upon entry into SA-β-gal expressing cells. Detailed facts importance specialist readers are published as "Supporting Information". Such documents...
Activation of energy expenditure in thermogenic fat is a promising strategy to improve metabolic health, yet the dynamic processes that evoke this response are poorly understood. Here we show synthesis mitochondrial phospholipid cardiolipin indispensable for stimulating and sustaining function. Cardiolipin biosynthesis robustly induced brown beige adipose upon cold exposure. Mimicking through overexpression synthase (Crls1) enhances consumption mouse human adipocytes. Crls1 deficiency...
Abstract The target of rapamycin complex 1 (TORC1) pathway couples nutrient, energy and hormonal signals with eukaryotic cell growth division. In yeast, TORC1 coordinates G –S cycle progression, also coined as START, by favouring the expression cyclins that activate cyclin-dependent protein kinases (CDKs) destabilizing CDK inhibitor Sic1. Following downregulation treatment or nutrient limitation, clearance C-terminal phosphorylation Sic1 unknown are both required for to escape...
One of the most widely used strategies for metabolite annotation in untargeted LCMS is based on analysis MSn spectra acquired using data-dependent acquisition (DDA), where precursor ions are sequentially selected from MS scans user-selected criteria. However, number that can be during a chromatogram limited and trade-off between analytical speed, sensitivity coverage must ensured. In this research, we compare four different automated MS2 DDA, which easily implemented frame standard QA/QC...
Toxicity studies, among them hepatotoxicity, are key throughout preclinical stages of drug development to minimise undesired toxic effects that might eventually appear in the course clinical use new drug. Understanding mechanism injury hepatotoxins is essential efficiently anticipate their potential risk toxicity humans. The vitro models and particularly cultured hepatocytes represents an easy robust alternative animal hepatotoxicity testing for predicting human risk. Here, we envisage...
This work was undertaken to address the potential environmental impact of UVA filter Uvinul® A Plus (DHHB) upon its biotransformation in humans. For this purpose, putative human metabolite 3 prepared by a three-step synthetic sequence involving initial Koenigs-Knorr reaction 2-(4-diethylamino-2-hydroxybenzoyl)benzoic acid (DHB) with acetobromo-α-D-glucuronic methyl ester, which afforded corresponding peracetylated DHB-acyl glucuronide (1). Subsequent enzymatic deprotection amano lipase (LAS)...
Abstract Eukaryotic cell cycle progression through G 1 –S is driven by hormonal and growth-related signals that are transmitted the target of rapamycin complex (TORC1) pathway. In yeast, inactivation TORC1 restricts transition due to rapid clearance cyclins (Cln) stabilization B-type cyclin (Clb) cyclin-dependent kinase (CDK) inhibitor Sic1. The latter mechanism remains mysterious but requires phosphorylation Sic1-Thr 173 Mpk1 Sic1-pThr -targeting phosphatase (PP2A Cdc55 ) greatwall...
Inflammatory bowel disease (IBD) is a chronic, relapsing noninfectious inflammatory condition of the intestinal tract with two main phenotypes, ulcerative colitis (UC) and Crohn's (CD), globally increasing incidence prevalence. Nearly 80% IBD patients active 50% those inactive suffer fatigue significant impairment their quality life. Fatigue has been associated multiple factors in but, most cases, no direct cause can be identified, risk clinically quiescent are contradictory. Furthermore, as...
Human milk (HM) is the gold standard for newborn nutrition. When own mother's not sufficiently available, pasteurized donor human becomes a valuable alternative. In this study we analyzed impact of Holder pasteurization (HoP) on metabolic and lipidomic composition HM. Metabolomic profiles twelve paired HM samples were analysed before after HoP by liquid chromatography-mass spectrometry (MS) gas chromatography-MS. Lipidomic analysis enabled annotation 786 features in out which 289...
The assessment of liver steatosis is crucial in both hepatology and transplantation (LT) surgery. Steatosis can negatively impact the success LT. a factor for excluding donated organs LT, but increasing demand transplantable has led to use from marginal donors. current standard evaluating semi-quantitative grading based on visual examination hematoxylin eosin (H&E)-stained biopsy, this method time-consuming, subjective, lacks reproducibility. Recent research shown that infrared (IR)...
Im Alter lernt man loszulassen: Die intrazelluläre kontrollierte Freisetzung von Molekülen in seneszierenden Zellen gelingt mithilfe mesoporösen Siliciumdioxidnanopartikeln (MSNs) mit einem Galactooligosaccharid(GOS)-Bezug, der die eingeschlossenen Moleküle (rote Kugeln; siehe Schema) den MSNs zurückhält. GOS ist ein Substrat des biologischen Markers Seneszenz-assoziierte β-Galactosidase (SA-β-gal), Freisetzungsvorgang beim Eintreten SA-β-gal exprimierende auslöst.
This review focuses on the latest developments in characterization of liver abnormalities using infrared (IR) or Fourier transform IR spectroscopy and microspectroscopy. Results from preclinical clinical studies show that these technological advances hold potential for identification abnormalities, including nonalcoholic fatty disease (NAFLD), fibrosis, tumors, through analysis body fluids tissues. The also discusses hyperspectral microscopy spectropathology identifies key factors would...
REACH (Registration, Evaluation, Authorization and Restriction of Chemicals) is a global strategy regulation policy the EU that aims to improve protection human health environment through better earlier identification intrinsic properties chemical substances. It entered into force on 1st June 2007 (EC 1907/2006). policies plead for use robust high-throughput "omic" techniques in vitro investigation toxicity chemicals can provide an estimation their hazards as well information regarding...
The vitamin D receptor (VDR) mediates 1,25-dihydroxyvitamin D3 pleiotropic biological actions through transcription regulation of target genes. expression levels this ligand-activated nuclear are regulated by multiple mechanisms both at transcriptional and post-transcriptional levels. Vitamin is the natural VDR activator, but other molecules signaling pathways have also been reported to regulate activity. In study, we identify valproic acid (VPA) short-chain fatty acids (SCFAs) as novel...
The estimation of steatosis in a liver graft is mandatory prior to transplantation, as the risk failure increases with level infiltrated fat. However, assessment before transplantation typically based on qualitative or semiquantitative characterization by visual inspection and palpation histological analysis. Thus, there an unmet need for surgeons have access diagnostic tool enabling situ fast classification grafts extraction. In this study, we assessed attenuated total reflection-Fourier...
Typical protocols to differentiate induced pluripotent stem cells (iPSCs) from hepatocyte-like (HLCs) imply complex strategies that include transfection with key hepatic transcription factors and the addition culture media of nutrients, growth factors, cytokines. A main constraint evaluate phenotype achieved arises way grade differentiation is determined. Currently, it relies on assessment expression a limited number gene transcripts, less frequently by assessing certain metabolic functions,...