A5072523148- Adipose Tissue and Metabolism
- Oral and Maxillofacial Pathology
- RNA modifications and cancer
- Olfactory and Sensory Function Studies
- Bone Tumor Diagnosis and Treatments
- Cancer-related gene regulation
- Circadian rhythm and melatonin
- Regulation of Appetite and Obesity
- Biochemical Analysis and Sensing Techniques
- Sleep and Wakefulness Research
- Liver Diseases and Immunity
- Adipokines, Inflammation, and Metabolic Diseases
- Tumors and Oncological Cases
- Sarcoma Diagnosis and Treatment
- Fatty Acid Research and Health
- Autoimmune and Inflammatory Disorders
- Dysphagia Assessment and Management
- Diet and metabolism studies
- Neurobiology and Insect Physiology Research
- Muscle Physiology and Disorders
- Gallbladder and Bile Duct Disorders
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Scoliosis diagnosis and treatment
Joslin Diabetes Center
2020-2025
Harvard University
2020-2025
Harvard Stem Cell Institute
2023-2025
Beth Israel Deaconess Hospital
2023-2025
Beth Israel Deaconess Medical Center
2023-2025
Osaka University
2013-2023
Matsunami General Hospital
2014-2017
Meijo Hospital
2012
Hokuto Hospital
2000
Nara City Hospital
1966
N6-methyladenosine (m6A) is among the most abundant mRNA modifications, yet its cell-type-specific regulatory roles remain unclear. Here we show that m6A methyltransferase-like 14 (METTL14) differentially regulates transcriptome in brown versus white adipose tissue (BAT and WAT), leading to divergent metabolic outcomes. In humans mice with insulin resistance, METTL14 expression differs significantly from BAT WAT context of correlation sensitivity. Mettl14-knockout promotes prostaglandin...
Abstract We developed MEBOCOST, a computational algorithm for quantitatively inferring metabolite-mediated intercellular communications using single cell RNA-seq data. The identifies cell-cell in which metabolites, such as lipids, are secreted by sender cells and traveled to interact with sensor proteins of receiver cells. on might be surface receptors, transporters across the membrane, or nuclear receptors. MEBOCOST relies comprehensive database metabolite-sensor partners, we manually...
Opsin3 (Opn3) is a transmembrane heptahelical G protein-coupled receptor (GPCR) with the potential to produce nonvisual photoreceptive effect. Interestingly, anatomical profiling of GPCRs reveals that Opn3 mRNA highly expressed in adipose tissue. The photosensitive functions mammals are poorly understood, and whether has role fat entirely unknown. In this study, we found Opn3-knockout (Opn3-KO) mice were prone diet-induced obesity insulin resistance. At cellular level, Opn3-KO brown...
Abstract Odors have been shown to exert an influence on various physiological and behavioral activities. However, little is known whether or not odor stimulation directly affects the levels of feeding-related neuropeptides. Here we show that neural transmission by Osmanthus fragrans (OSM) decreased mRNA expression orexigenic neuropeptides, such as agouti-related protein, neuropeptide Y, melanin-concentrating hormone prepro-orexin, while increased anorexigenic cocaine- amphetamine-regulated...
Introduction The enhanced β-cell senescence that accompanies insulin resistance and aging contributes to cellular dysfunction loss of transcriptional identity leading type 2 diabetes (T2D). While is among the 12 recognized hallmarks aging, its relation other including altered nutrient sensing (insulin/IGF1 pathway) in β-cells not fully understood. We previously reported an increased expression IGF1R mouse human a marker older β-cells; however, contribution age-related remains be determined....
To investigate the levels of neuron-specific enolase (NSE) in serum and cerebrospinal fluid (CSF) patients with early stage Creutzfeldt-Jakob disease (CJD).The NSE CSF were examined 6 cases CJD patients. The measured 8 age matched control other neurological diseases also another diseases. groups consisted 1 same patient 7 different 2 both for CSF.The level (17.3 +/- 7.0 ng/ml, mean SD) was significantly higher than that controls (6.5 1.6) (P < 0.02) as case (79.3 53.3 ng/ml) vs (9.6 2.9)...
The orexins (orexin-A and orexin-B) are neuropeptides that secreted from neurons in the lateral hypothalamus participate regulation of feeding behavior. It remains to be determined, however, how exert their effects on behavior, including masticatory movements. To this end, we analyzed food intake behavior muscle activity using video analysis electromyography (EMG) recording methods. results showed cumulative over 4 h was larger rats intraventricularly injected with either orexin-A or...
N6-methyladenosine (m6A) is among the most abundant post-transcriptional modifications in mRNA, yet its precise cell-type-specific roles orchestrating gene expression programs remain elusive. Although brown and white adipocytes are both considered fat cells, their unique properties underscore importance of context-specific regulation for whole-body metabolism. Here, we provide first evidence that m6A methyltransferase-like 14 (METTL14) differentially regulates transcriptomes adipose tissue...
Thermogenic adipose tissue, consisting of brown and beige fat, regulates nutrient utilization energy metabolism. Human fat is relatively scarce decreases with obesity aging. Hence, inducing thermogenic differentiation white offers an attractive way to enhance whole-body metabolic capacity. Here, we show the role endothelin 3 (EDN3) receptor type B (EDNRB) in promoting browning tissue (WAT). EDNRB overexpression stimulates human preadipocytes through cAMP-EPAC1-ERK activation. In mice, cold...
Peripheral ameloblastomas (PAs) of the extragingival areas are extremely rare. To best our knowledge, only five cases PA have been reported. We present here a sixth case buccal mucosa in an 80‐year‐old male. The tumor was surgically removed by blunt dissection and there is no evidence recurrence for 7 months. also discuss clinical characteristics, origin, management reference to relevant literature.
A balanced omega (ω)-6/ω-3 polyunsaturated fatty acids (PUFAs) ratio has been linked to metabolic health and the prevention of chronic diseases. Brown adipose tissue (BAT) specializes in energy expenditure secretes signaling molecules that regulate metabolism via inter-organ crosstalk. Recent studies have uncovered BAT produces different PUFA species circulating oxylipin levels are correlated with BAT-mediated mice humans. However, impact ω-6/ω-3 PUFAs on phenotype not fully elucidated. The...
Brown adipose tissue (BAT) is an depot specialized in energy dissipation that can also serve as endocrine organ via the secretion of bioactive molecules. The creation BAT-specific knockout mice one most popular approaches for understanding contribution a gene interest to BAT-mediated regulation. conventional targeting strategy utilizing Cre-LoxP system has been principal approach generate tissue-specific mice. However, this time-consuming and tedious. Here, we describe protocol rapid...
In both humans and animals, chemosensory stimuli, including odors tastes, induce a variety of physiologic mental responses related to energy homeostasis, such as glucose kinetics. The present study examined the importance olfactory function in kinetics following ingestion behavior simplified experimental scenario. We applied conventional tolerance test rats with without analyzed subsequent blood (BG) curves detail. loss input due damage mucosa induced marked decrease area under BG curve....